PTP1B phosphatase as a novel target of oleuropein activity in MCF-7 breast cancer model.

6533b82afe1ef96bd128c37e

RESEARCH PRODUCT

PTP1B phosphatase as a novel target of oleuropein activity in MCF-7 breast cancer model.

Alicja Kuban-jankowska Fabrizio Lo Celso Tomasz Kostrzewa Giampaolo Barone Giosuè Lo Bosco Anna Kamm Magdalena Gorska-ponikowska Magdalena Gorska-ponikowska Paulina Przychodzen Agnieszka Daca Roksana Wyszkowska

subject

0301 basic medicine Cell cycle checkpoint Immunoprecipitation Cell Survival medicine.medical_treatment Phosphatase Iridoid Glucosides Antineoplastic Agents Breast Neoplasms Adenocarcinoma Molecular Dynamics Simulation Toxicology Flow cytometry 03 medical and health sciences chemistry.chemical_compound breast cancer 0302 clinical medicine Breast cancer Oleuropein medicine Humans PTP1B phosphatase Iridoids skin and connective tissue diseases Settore CHIM/02 - Chimica Fisica Cell Proliferation Oleuropein Protein Tyrosine Phosphatase Non-Receptor Type 1 MCF-7 cell medicine.diagnostic_test Anticancer therapy General Medicine medicine.disease 030104 developmental biology chemistry MCF-7 Settore CHIM/03 - Chimica Generale E Inorganica 030220 oncology & carcinogenesis Settore BIO/14 - Farmacologia Cancer research MCF-7 Cells Adjuvant hormones hormone substitutes and hormone antagonists

description

Phosphatase PTP1B has become a therapeutic target for the treatment of type 2-diabetes, whereas recent studies have revealed that PTP1B plays a pivotal role in pathophysiology and development of breast cancer. Oleuropein is a natural, phenolic compound with anticancer activity. The aim of this study was to address the question whether PTP1B constitutes a target for oleuropein in breast cancer MCF-7 cells. The cellular MCF-7 breast cancer model was used in the study. The experiments were performed using cellular viability tests, Elisa assays, immunoprecipitation, flow cytometry analyses and computer modelling. Herein, we evidenced that the reduced activity of phosphatase PTP1B after treatment with oleuropein is strictly correlated with decreased MCF-7 cellular viability and cell cycle arrest. These results provide new insight into further research on oleuropein and possible role of the compound in adjuvant treatment of breast cancer.

year	journal	country	edition	language
2019-06-11	Toxicology in vitro : an international journal published in association with BIBRA

10.1016/j.tiv.2019.104624 https://pubmed.ncbi.nlm.nih.gov/31419504