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RESEARCH PRODUCT
Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.
Rizzo ManfrediFrancisco MesaNavidah ChaudharyFrancisco O'valleLuigi NibaliRicardo F. MuñozMohamed ParkarFrancesco CariniFrancesco CappelloNikos Donossubject
Bacterial DiseasesMaleBiopsyGingivaDentistryGene ExpressionPilot ProjectsEpitheliumMonocytesGingivitisGenotypehealth care economics and organizationsPlasma cellsMultidisciplinaryGingival AbscessesbiologyQRMiddle AgedGingivitishumanitiesmedicine.anatomical_structureInfectious DiseasesCOX-2 6681231 genotype interleukin-6 periodontitisCytokinesPeriodontal AbscessesMedicineFemalemedicine.symptomPeriodontal IndexConnective tissueImmunohistochemical AnalysisResearch ArticleAdultmedicine.medical_specialtyClinical Research DesignScienceOral MedicineConnective tissueHemorrhagePolymorphism Single NucleotideInternal medicinemedicineGeneticsHumansInterleukin 6PeriodontitisBiologyAgedPeriodontitisClinical GeneticsInflammationbusiness.industryInterleukin-6Case-control studymedicine.diseaseChronic periodontitisHaplotypesCyclooxygenase 2Immune SystemCase-Control StudiesChronic Periodontitisbiology.proteinGenetic PolymorphismImmunologic TechniquesClinical ImmunologybusinessPopulation Geneticsdescription
[Objectives] The aim of this pilot study was to investigate associations between IL-6 and COX-2 expression in gingival biopsies and both clinical diagnosis and genotypes in the IL-6 and COX-2 genes. [Design] A case-control study included 41 gingival biopsies obtained from Caucasian patients grouped according to clinical diagnosis of gingival health (n = 10), gingivitis (n = 15) or chronic periodontitis (n = 16). Immunohistochemistry analyses were performed to determine COX-2 expression in lamina propria, IL-6 expression in lamina propria and gingival epithelium and level of inflammatory cell infiltrate. Individual DNA was extracted and genotyped by real-time PCR for IL6 SNPs rs 2069827 and rs 2069825 and for COX-2 rs 6681231. [Results] The percentage of cellular COX-2 expression was associated with the extent of periodontal disease (Arbes index p = 0.026) and inflammatory infiltrate (p<0.0001). No association was observed between IL6 haplotypes and cells positive to IL-6 or COX-2 in gingival tissues. The COX-2 rs 6681231 was associated with cells positive to IL-6 in the connective tissue (p = 0.032). [Conclusions] COX-2 expression in gingival tissues may be a marker of periodontal disease severity. COX-2 rs 6681231 may be associated with IL-6 local production in gingival tissues.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-01-01 | PLoS ONE |