6533b82efe1ef96bd1293df3

RESEARCH PRODUCT

Hsp60 and Hsp10 increase in colon mucosa of Crohn's disease and ulcerative colitis.

Sabrina DavidEverly Conway De MacarioGiovanni ZummoGiovanni TomaselloAlessandro PitruzzellaSalvatore AccomandoAnna MartoranaVito RodolicoAntonella Marino GammazzaMonica ZerilliAlberto J.l. MacarioProvvidenza DamianiFrancesco Cappello

subject

AdultMaleColonConnective tissueInflammationBiologyBiochemistryImmune systemSettore MED/38 - Pediatria Generale E SpecialisticaCrohn DiseaseIntestinal mucosaChaperonin 10LeukocytesmedicineHumansIntestinal MucosaColitisIBD HSP 60 HSP 10AgedOriginal PaperLamina propriaCrohn's diseaseEpithelial CellsChaperonin 60Cell BiologyMiddle Agedmedicine.diseaseUlcerative colitismedicine.anatomical_structureImmunologyColitis UlcerativeFemalemedicine.symptom

description

The purpose of this work was to determine in colon mucosa of Crohn’s disease (CD) and ulcerative colitis (UC) in relapse: a) the levels of the chaperonins Hsp60 and Hsp10; b) the quantity of inflammatory cells; and c) if the levels of chaperonins parallel those of inflammation cells. Twenty cases of CD and UC and twenty normal controls (NC) were studied using immunohistochemistry, Western blotting and immunofluorescence. Immunohistochemically, Hsp60 and Hsp10 were increased in both inflammatory bowel diseases (IBD) compared to NC. These results were confirmed by Western blotting. Hsp60 and Hsp10 occurred in the cytoplasm of epithelial cells in CD and UC but not in NC. Hsp60 and Hsp10 co-localised to epithelial cells of mucosal glands but not always in connective tissue cells of lamina propria, where only Hsp60 or, less often, Hsp10 was found. Cells typical of inflammation were significantly more abundant in CD and UC than in NC. Since chaperonins are key factors in the activation of the immune system leading to inflammation, we propose that they play a central role in the pathogenesis of the two diseases, which, consequently, ought to be studied as chaperonopathies.

yearjournalcountryeditionlanguage
2010-04-15
10.1007/s12192-010-0196-8http://hdl.handle.net/10447/49474