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RESEARCH PRODUCT
Elevated levels of serum-soluble triggering receptor expressed on myeloid cells-1 in patients with IBD do not correlate with intestinal TREM-1 mRNA expression and endoscopic disease activity.
Saurer LeslieRihs SilviaBirrer MichèleSaxer-seculic NikolinaMarkus P. RadsakMueller ChristophSwiss Ibd Cohort Studysubject
AdultMalemedicine.medical_specialtyMyeloidColonGastroenterologyInflammatory bowel diseaseEndoscopy GastrointestinalStatistics Nonparametric03 medical and health sciencesMice0302 clinical medicineIntestinal mucosaCrohn DiseaseIleumInternal medicinemedicineAnimalsHumansRNA MessengerColitisReceptors ImmunologicReceptor030304 developmental biology0303 health sciencesCrohn's diseaseMessenger RNAMembrane Glycoproteinsbusiness.industryGastroenterologyGeneral MedicineMiddle Agedmedicine.diseaseUlcerative colitisAdoptive Transferdigestive system diseasesTriggering Receptor Expressed on Myeloid Cells-13. Good healthmedicine.anatomical_structureROC CurveArea Under CurveImmunologyColitis UlcerativeFemalebusinessBiomarkers030215 immunologydescription
BACKGROUND AIMS Triggering receptor expressed on myeloid cells 1 (TREM 1) is a potent amplifier of pro inflammatory responses. We have previously demonstrated a substantial increase in TREM 1 expressing macrophages in the inflamed intestinal mucosa of patients with inflammatory bowel diseases (IBD). TREM 1 is also produced as a soluble receptor (sTREM 1). Here we aimed to determine whether serum sTREM 1 could be used as a surrogate marker of disease activity in patients with IBD. METHODS Intestinal biopsies and concurrently collected sera from patients with Crohn's disease (CD) and Ulcerative colitis (UC) enrolled in the Swiss IBD cohort study were analyzed for intestinal TREM 1 mRNA and serum sTREM 1 expression. TREM 1 mRNA and sTREM 1 were correlated with the endoscopically determined disease activity. Serum sTREM 1 and TREM 1 mRNA expression levels were further determined in sera and colonic tissues collected at various time points post disease induction in an experimental mouse model of colitis and correlated with disease activity. RESULTS Expression of TREM 1 mRNA was upregulated in intestinal biopsies from patients with active disease but not in patients with quiescent disease. Serum sTREM 1 was elevated in IBD patients compared to normal controls. No substantial differences in sTREM 1 expression levels were found in patients with active versus quiescent disease. In colitic mice colonic TREM 1 mRNA and serum sTREM 1 were also upregulated. While colonic TREM 1 mRNA expression levels correlated with disease activity augmented serum sTREM 1 in fact associated with a milder course of disease. CONCLUSIONS Analysis of sTREM 1 as a surrogate marker of disease activity in patients with IBD warrants caution.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-10-01 | Journal of Crohn's & colitis |