6533b82ffe1ef96bd129536c
RESEARCH PRODUCT
Clastogenic and aneuploidizing effects of antiblastic busulphan revealed by kinetochore immunofluorescence in CHO cells.
G. GranataA. AnzaloneGiusi BarbataM.c. GiglioP. Carbonesubject
CentromereAneuploidyFluorescent Antibody TechniqueBiologyImmunofluorescenceCell LineAcetoneClastogenhemic and lymphatic diseasesmedicineHumansBusulfanMicronuclei Chromosome-DefectiveChromosome AberrationsMicronucleus TestsScleroderma Systemicmedicine.diagnostic_testDose-Response Relationship DrugGeneral Medicinemedicine.diseaseAneuploidyMolecular biologyIn vitroCell cultureMicronucleus testMicronucleusBusulfanmedicine.drugdescription
We utilized, in CHO cells, the cytoplasm preservation technique to evaluate the micronucleus frequency at different busulphan concentrations, and the indirect immunofluorescence technique, using sera obtained from patients with scleroderma (CREST variant), to analyze if busulphan-induced micronuclei have kinetochores. Results show that this alkylating agent is capable of causing a significant increase of micronuclei in vitro, a great part (40%) of them having CREST-positive kinetochores. These findings confirm the clastogenic effect of busulphan and reveal a considerable capability of this agent to induce aneuploidy. These results are examined taking into account the high incidence of secondary neoplasias induced by chemotherapy with alkylating agents utilized against primary neoplasias.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-08-01 | Mutation research |