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RESEARCH PRODUCT
More about sampling and estimation of mercaptans in air samples
Miquel Avella-oliverYolanda Moliner-martínezCarmen Molins-leguaPilar Campíns-falcóRosa Herráez-hernándezJorge Verdú-andréssubject
AnalyteSorbentAir samplesAlkaliesHigh-performance liquid chromatographyAnalytical ChemistryCartridgeO-Phthalaldehydechemistry.chemical_compoundMercaptansQUIMICA ANALITICAHumansSulfhydryl CompoundsIsoleucineDerivatizationAir sampling systemFluorescent DyesAir PollutantsChromatographyAirSampling (statistics)DerivatizationSolutionsSpectrometry FluorescencechemistryReagentCalibrationHPLCo-PhthalaldehydeChromatography Liquiddescription
[EN] Several strategies have been developed for sampling and determination of volatile thiols. The selectivity and sensitivity of the proposed methodologies are achieved by using a specific derivatizing reagent. The different procedures assayed are based on air sampling followed by derivatization of the analytes with OPA and isoleucine in alkaline solution. The derivatization products are separated and determined by liquid chromatography and fluorescence detection. To start, the derivatization conditions and stability of the derivates have been studied in order to establish the storage conditions. In general, the strategies studied consisted on trapping and detivatization the thiol compound on different support; a solution (Impinger) or sorbent (C-18 cartridges or glass fiber filter). The analytical properties of the different strategies have been obtained and compared. Procedures are recommended upon specific situations. (C) 2012 Elsevier B.V. All rights reserved.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-03-01 |