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RESEARCH PRODUCT
Thermal restraint of a bacterial exopolysaccharide of shallow vent origin
Concetta GugliandoloSalvatore MagazùVincenzo ZammutoVincenzo ZammutoClaire Madeleine-perdrillatMaria Teresa CaccamoAntonio Spanòsubject
Materials science[SDV]Life Sciences [q-bio]02 engineering and technologyengineering.material010402 general chemistry01 natural sciencesBiochemistrySpectral linesymbols.namesakeHydrothermal VentsStructural BiologyBacillus licheniformisThermal stabilityBacillus licheniformisThermal analysisSpectroscopyMolecular BiologybiologyPolysaccharides BacterialGeneral Medicine021001 nanoscience & nanotechnologybiology.organism_classification0104 chemical sciences[SDV] Life Sciences [q-bio]Fourier transformChemical engineeringAttenuated total reflectionAttenuated-Total-Reflectance Infra-Red spectroscopy Extremophiles Spectral distance Thermal analysis Wavelet cross-correlation analysisengineeringsymbolsBiopolymer0210 nano-technologydescription
This is the post-print version of the following article: "Thermal restraint of a bacterial exopolysaccharide of shallow vent origin", which has been published in final form at https://www.sciencedirect.com/science/article/pii/S0141813018308250?via%3Dihub; International audience; To dynamically characterize the thermal properties of the fructose-rich exopolysaccharide (EPS1-T14), produced by themarine thermophilic Bacillus licheniformis T14, the Attenuated Total Reflectance Fourier Transform Infra-Redspectroscopy was coupled to variable temperature ranging from ambient to 80 °C.The spectra were analyzed by the following innovative mathematical tools: i) non-ideal spectral deviation, ii) OHstretchingband frequency center shift, iii) spectral distance, and iv) wavelet cross-correlation analysis.The thermal restraint analysis revealed that the whole EPS1-T14 system possessed high stability until 80 °C, andsuggested that fucose was mainly involved in the EPS1-T14 thermal stability, whereas glucose was responsible for itsmolecular flexibility.Our results provide novel insights into the thermal stability properties of the whole EPS1-T14 and into the role of itsmain monosaccharidic units. As a new biopolymer, the thermostable EPS1-T14 could be used in traditionalbiotechnology fields and in new biomedical areas, as nanocarriers, requiring high temperature processes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-07-01 |