6533b836fe1ef96bd12a0b8e
RESEARCH PRODUCT
SiPM as miniaturised optical biosensor for DNA-microarray applications
M. F. SantangeloM. F. SantangeloE. L. SciutoA. C. BusaccaS. PetraliaS. ConociS. Libertinosubject
Detection limitAnalytePhotomultiplierMaterials scienceoptical biosensorbusiness.industrySiPMDNA microarrayPhotodetectorLinearityDNA-microarraySignalSettore ING-INF/01 - ElettronicaElectronic Optical and Magnetic MaterialsSilicon photomultiplierOpticslcsh:TA1-2040DNA microarray; Fluorophore detection; Optical Biosensor; SiPMSignal ProcessingElectrical and Electronic Engineeringbusinesslcsh:Engineering (General). Civil engineering (General)Sensitivity (electronics)Fluorophore detectionBiotechnologydescription
A miniaturized optical biosensor for low-level fluorescence emitted by DNA strands labelled with CY5 is showed. Aim of this work is to demonstrate that a Si-based photodetector, having a low noise and a high sensitivity, can replace traditional detection systems in DNA-microarray applications. The photodetector used is a photomultiplier (SiPM), with 25 pixels. It exhibits a higher sensitivity than commercial optical readers and we experimentally found a detection limit for spotted dried samples of ∼1 nM. We measured the fluorescence signal in different operating conditions (angle of analysis, fluorophores concentrations, solution volumes and support). Once fixed the angle of analysis, for samples spotted on Al-TEOS slide dried, the system is proportional to the concentration of the analyte in the sample and is linear in the range 1 nM–1 μM. For solutions, the range of linearity ranges from 100 fM to 10 nM. The system potentialities and the device low costs suggest it as basic component for the design and fabrication of a cheap, easy and portable optical system. Keywords: Optical Biosensor, SiPM, DNA microarray, Fluorophore detection
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-12-01 | Sensing and Bio-Sensing Research |