6533b839fe1ef96bd12a64d4
RESEARCH PRODUCT
Optimization of fluorescence enhancement for silicon-based microarrays
Vanessa MarinoClelia GalatiC. Arnonesubject
Quality ControlSiliconMaterials scienceSiliconBiomedical Engineeringchemistry.chemical_elementSubstrate (electronics)computer.software_genreSensitivity and SpecificitySettore ING-INF/01 - ElettronicaFluorescence spectroscopyBiomaterialsOpticsPlanarInterference (communication)Computer Aided DesignDetection theorySensitivity (control systems)Microscopy Confocalbusiness.industryoptical biosensingReproducibility of ResultsEquipment DesignImage EnhancementMicroarray AnalysisAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsEquipment Failure AnalysisMicroscopy FluorescencechemistryComputer-Aided Designbusinesscomputerdescription
An optical technique for the enhancement of fluorescence detection sensitivity on planar samples is presented. Such a technique is based on the simultaneous optimization of excitation and light collection by properly combining interference and reflectance from the sample holder. Comparative tests have been performed in microarray applications, by evaluating the proposed solution against commercial glass-based devices, using popular labeling dyes, such as Cy3 and Cy5. The proposed technique is implemented on a substrate built with standard silicon technology and is therefore well suited for integrated micro total analysis systems (microTAS) applications.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-11-22 | Journal of Biomedical Optics |