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RESEARCH PRODUCT

Impaired border zone formation and adverse remodeling after reperfused myocardial infarction in cannabinoid CB2 receptor deficient mice.

Alexandra M. KleinJan C. HeinemannMarkus VeltenWilhelm RoellChristopher GestrichAna KilićGeorg D. DuerrLaura BindilaKatharina KeppelOliver DewaldAndreas ZimmerBeat LutzTimo KlaasTobias Heuft

subject

medicine.medical_specialtyIschemiaMyocardial InfarctionInfarctionMyocardial Reperfusion InjuryGeneral Biochemistry Genetics and Molecular BiologyReceptor Cannabinoid CB2MiceInternal medicinemedicineCannabinoid receptor type 2AnimalsMyocytes CardiacMyocardial infarctionGeneral Pharmacology Toxicology and PharmaceuticsCardioprotectionInflammationMice KnockoutbiologyChemistryMyocardiumTenascin CHemodynamicsGranulation tissueGeneral Medicinemedicine.diseaseEndocannabinoid systemMice Inbred C57BLEndocrinologymedicine.anatomical_structureCardiologybiology.proteinGranulation TissueCytokines

description

Abstract Aims Reperfusion of myocardial infarction is associated with inflammatory reaction and subsequent myocardial remodeling with a rapid scar formation in mice. The cannabinoid receptor CB2 has been associated with cardioprotection and regulation of macrophage function. We investigated its role in remodeling of reperfused infarction. Main methods One hour LAD-occlusion was followed by reperfusion over 6 h and 1, 3 and 7 days in wild-type C57/BL6J (WT) and CB2 receptor-deficient (Cnr2 −/− ) mice (n = 8/group). Hearts were processed for functional, morphological and mRNA/protein analysis, and tissue concentration of endocannabinoids was determined using liquid chromatography-multiple reaction monitoring. Key findings In contrast to a rapid formation of granulation tissue and a compacted non-transmural scar in WT mice after 7 days of reperfusion, Cnr2 −/− mice showed a non-compacted transmural scar. Millar® left ventricular catheter measurements revealed a significantly worse function in Cnr2 −/− mice. We found no compensatory elevation of endocannabinoid concentration in Cnr2 −/− hearts. Macrophage infiltration was significantly stronger in Cnr2 −/− hearts and affected also the remote septum, when compared to WT hearts. We found a cytokine-driven inflammatory response in Cnr2 −/− hearts with no significant induction of chemokines. Immunohistochemistry for thrombospondin-1 revealed a dysfunctional infarction border zone formation in Cnr2 −/− hearts. Cnr2 −/− hearts showed no significant induction of tenascin C, collagen-Iα or lysil oxidase, thereby indicating adverse myocardial remodeling. Significance Endocannabinoids act via CB2 receptor in the modulation of inflammatory response and myocardial remodeling after infarction. CB2 receptor plays an important role in the formation of infarction border zone, collagen deposition and organization of stable scar during remodeling.

yearjournalcountryeditionlanguage
2014-09-30Life sciences
10.1016/j.lfs.2014.11.005https://pubmed.ncbi.nlm.nih.gov/25447445