6533b852fe1ef96bd12aa469
RESEARCH PRODUCT
Transformation of Amorphous Polyphosphate Nanoparticles into Coacervate Complexes: An Approach for the Encapsulation of Mesenchymal Stem Cells.
Rafael Muñoz-espíEmad TolbaMeik NeufurthXiaohong WangShunfeng WangWerner E. G. M�llerIngo LieberwirthHeinz C. SchröderGunnar GlasserMaximilian Ackermannsubject
0301 basic medicineNanoparticle02 engineering and technologyengineering.materialRegenerative Medicinelaw.inventionBiomaterials03 medical and health scienceschemistry.chemical_compoundlawPolyphosphatesotorhinolaryngologic diseasesZeta potentialAnimalsHumansGeneral Materials ScienceCoacervatePolyphosphateMesenchymal stem cellMesenchymal Stem CellsGeneral Chemistry021001 nanoscience & nanotechnologydigestive system diseases3. Good healthAmorphous solidInorganic PyrophosphataseMicroscopy Electronsurgical procedures operative030104 developmental biologychemistryengineeringBiophysicsNanoparticlesBiopolymerElectron microscope0210 nano-technologyBiotechnologydescription
Inorganic polyphosphate [polyP] has proven to be a promising physiological biopolymer for potential use in regenerative medicine because of its morphogenetic activity and function as an extracellular energy-donating system. Amorphous Ca2+ -polyP nanoparticles [Ca-polyP-NPs] are characterized by a high zeta potential with -34 mV (at pH 7.4). This should contribute to the stability of suspensions of the spherical nanoparticles (radius 94 nm), but make them less biocompatible. The zeta potential decreases to near zero after exposure of the Ca-polyP-NPs to protein/peptide-containing serum or medium plus serum. Electron microscopy analysis reveals that the particles rapidly change into a coacervate phase. Those mats are amorphous, but less stable than the likewise amorphous Ca-polyP-NPs and are morphogenetically active. Mesenchymal stem cells grown onto the polyP coacervate show enhanced growth/proliferation and become embedded in the coacervate. These results suggest that the Ca-polyP coacervate, formed from Ca-polyP-NPs in the presence of protein, can act as an adaptable framework that mimics a niche and provides metabolic energy in bone/cartilage engineering.
year | journal | country | edition | language |
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2018-05-30 | Small (Weinheim an der Bergstrasse, Germany) |