Diabetes-induced changes in endothelial mechanisms implicated in rabbit carotid arterial response to 5-hydroxytryptamine

6533b853fe1ef96bd12acbc6

RESEARCH PRODUCT

Diabetes-induced changes in endothelial mechanisms implicated in rabbit carotid arterial response to 5-hydroxytryptamine

Francisco J. Miranda José A. Alabadí Enrique Alborch Rosa F Ruiz De Apodaca Silvia Llorens José M. Centeno

subject

Male Serotonin medicine.medical_specialty Contraction (grammar)Arginine Endothelium Indomethacin In Vitro Techniques Guanidines Nitroarginine Diabetes Mellitus Experimental Nitric oxide chemistry.chemical_compound Internal medicine Diabetes mellitus medicine Animals Enzyme Inhibitors Pharmacology Lagomorpha Dose-Response Relationship Drug biology Vascular disease business.industry Prostanoid medicine.disease biology.organism_classification Carotid Arteries medicine.anatomical_structure Endocrinology chemistry Vasoconstriction Ketamine Endothelium Vascular Rabbits Nitric Oxide Synthase business

description

Abstract The influence of diabetes on endothelial mechanisms implicated in the response of isolated rabbit carotid arteries to 5-hydroxytryptamine (5- HT ) was studied. 5-HT induced a concentration–dependent contraction that was potentiated in arteries from diabetic rabbits with respect to that in arteries from control rabbits. Endothelium removal potentiated 5-HT contractions in arteries from both control and diabetic rabbits but increased the maximum effect only in arteries from diabetic rabbits. Incubation of arterial segments with N G -nitro- l -arginine ( l- NA) enhanced the contractile response to 5-HT. This l -NA enhancement was greater in arteries from diabetic rabbits than in arteries from control rabbits. Aminoguanidine did not modify the 5-HT contraction in arteries from control and diabetic rabbits. Indomethacin inhibited the 5-HT-induced response, and this inhibition was higher in arteries from control rabbits than in arteries from diabetic rabbits. In summary, diabetes enhances the sensitivity of the rabbit carotid artery to 5-HT. In control animals, the endothelium modulated the arterial response to 5-HT by the release of both nitric oxide (NO) and a vasoconstrictor prostanoid. Diabetes enhances endothelial constitutive NO activity and impairs the production of the endothelial vasoconstrictor.

year	journal	country	edition	language
2000-08-01	European Journal of Pharmacology

https://doi.org/10.1016/s0014-2999(00)00469-6