6533b854fe1ef96bd12ae97a
RESEARCH PRODUCT
Human brain organoids assemble functionally integrated bilateral optic vesicles
Ata Alp SurenGuobin BaoArgyris PapantonisAnand RamaniAruljothi MariappanWalid AlbannaWalid AlbannaSilvio O. RizzoliKerstin Nagel-wolfrumOlivier GoureauToni SchneiderMaria Giovanna RiparbelliVolker BusskampFriedrich SchinzelElke GabrielMarco GottardoJürgen HeschelerGiovanni PasquiniNatasa JosipovicNatasa JosipovicVeronica PersicoJay GopalakrishnanJanine AltmüllerCeleste M. KarchGiuliano Callainisubject
OrganogenesisInduced Pluripotent Stem Cellsretinal pigment epitheliumiPSCsEmbryonic DevelopmentBiology03 medical and health sciencesDiencephalonchemistry.chemical_compoundProsencephalon0302 clinical medicineGeneticsOrganoidmedicineHumansInduced pluripotent stem cell030304 developmental biology0303 health sciencesforebrain organoidsRetinal pigment epitheliumbrain organoidsVesicleprimordial eye fieldsOVB-organoidsCell DifferentiationRetinalCell BiologyOptic vesicleHuman brainCell biologyOrganoidsmedicine.anatomical_structurenervous systemchemistryMolecular MedicineFOXG1; OVB-organoids; brain organoids; forebrain organoids; iPSCs; optic vesicles; primary cilium; primordial eye fields; retinal pigment epitheliumoptic vesiclesFOXG1030217 neurology & neurosurgeryprimary ciliumdescription
During embryogenesis, optic vesicles develop from the diencephalon via a multistep process of organogenesis. Using induced pluripotent stem cell (iPSC)-derived human brain organoids, we attempted to simplify the complexities and demonstrate formation of forebrain-associated bilateral optic vesicles, cellular diversity, and functionality. Around day 30, brain organoids attempt to assemble optic vesicles, which develop progressively as visible structures within 60 days. These optic vesicle-containing brain organoids (OVB-organoids) constitute a developing optic vesicle's cellular components, including primitive corneal epithelial and lens-like cells, retinal pigment epithelia, retinal progenitor cells, axon-like projections, and electrically active neuronal networks. OVB-organoids also display synapsin-1, CTIP-positive myelinated cortical neurons, and microglia. Interestingly, various light intensities could trigger photosensitive activity of OVB-organoids, and light sensitivities could be reset after transient photobleaching. Thus, brain organoids have the intrinsic ability to self-organize forebrain-associated primitive sensory structures in a topographically restricted manner and can allow interorgan interaction studies within a single organoid.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-10-01 | Cell Stem Cell |