6533b854fe1ef96bd12aea86
RESEARCH PRODUCT
Sterol and ecdysteroids profiles of Serratula tinctoria (L.) : plant and cell cultures producing steroids
L. ChapuisJ.f. MouilletJean-paul DelbecqueMarie-france Corio-costetsubject
0106 biological sciencesmedicine.medical_treatment[SDV]Life Sciences [q-bio]LathosterolBiology01 natural sciencesBiochemistrySteroidTerpene03 medical and health scienceschemistry.chemical_compoundSerratulapolycyclic compoundsmedicineMolecular BiologyComputingMilieux_MISCELLANEOUS030304 developmental biology0303 health sciencesEcdysteroidCholesterolfungibiology.organism_classificationSterolSterol esterificationchemistryBiochemistryInsect Sciencelipids (amino acids peptides and proteins)010606 plant biology & botanydescription
Abstract Cell suspension cultures have been obtained from Serratula tinctoria, a plant producing ecdysteroids. Sterol profiles and ecdysteroid contents have been analysed and compared in plants and cell cultures. In particular, the composition of free and esterified sterols was investigated using appropriate analytical techniques. In plants, esterified sterols were more abundant (50–70% of the total sterol) than in cell cultures (13–36%). A selectivity for sterol esterification was noted: in plants, the triterpenes (as amyrins) were esterified, whereas it was the 4-desmethylsterols (sitosterol and cholesterol) in cell cultures. Ecdysteroids were present in higher quantities in plant (0.1–1.2% dry wt) than in cell cultures (0.01–0.03%). Analysis confirmed the presence of 20-hydroxyecdysone, 20-hydroxyecdysone-3-acetate and polypodine B as the main compounds. Roots were not only the richest organ in ecdysteroids, but also in cholesterol, lathosterol and 24-methylene-cholesterol. The results obtained suggest interesting relationships between free or esterified sterols and ecdysteroid contents.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-01-01 |