6533b856fe1ef96bd12b1d12
RESEARCH PRODUCT
Endoribonuclease IV. A poly(A)-specific ribonuclease from chick oviduct. 1. Purification of the enzyme.
Werner E. G. M�llersubject
EndoribonucleaseOviductsBiologyBiochemistryCofactorStructure-Activity RelationshipRibonucleasesAnimalsMagnesiumchemistry.chemical_classificationNucleaseManganeseOligoribonucleotidesOligonucleotideEndoribonuclease IVEndonucleasesMolecular biologyEnzyme ActivationMolecular WeightKineticsEnzymeBiochemistrychemistryYield (chemistry)biology.proteinOviductFemalePoly AChickensdescription
A new endoribonuclease, termed endoribonuclease IV, has been described. This enzyme has been isolated from chick oviducts and purified 15 000-fold in a 25% yield nearly to homogeneity. The nuclease, which specifically degrades poly(A), forms oligonucleotides of an average chain length of 10. These (A)-10 fragments are terminated by 3'-hydroxyl and 5'-phosphate groups. The enzyme has a pH optimum at 8.7, requires Mn2+ or Mg2+ as a cofactor, and has a molecular weight of about 45 000.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1976-11-01 | European journal of biochemistry |