6533b856fe1ef96bd12b3290
RESEARCH PRODUCT
Germ-free housing conditions do not affect aortic root and aortic arch lesion size of late atherosclerotic low-density lipoprotein receptor-deficient mice
Thomas KoeckPhilipp S. WildGiulia PontarolloSven JäckelChristian WeberYvonne JansenCornelia KarwotYvonne DöringYvonne DöringWolfram RufEmiel P. C. Van Der VorstAndreas DaiberKlytaimnistra KiouptsiChristoph ReinhardtSusanne GerberJohannes BraunAngelica KarpiHristo TodorovFranziska Bayersubject
0301 basic medicineAortic archMalePathologyaortic rootAortic rootaortic archFunctional impactAorta ThoracicHYPERCHOLESTEROLEMIAMice0302 clinical medicineDeficient mouse610 Medicine & healthMice KnockoutBILE-ACIDSCellular compositionMicrobiotaCHOLESTEROLGUT MICROBIOTAGastroenterologyinflammatory markersHousing AnimalPlaque Atheroscleroticmacrophagessmooth muscle cellsInfectious Diseasesgerm-free030211 gastroenterology & hepatologyFemalelipids (amino acids peptides and proteins)SEXTRIMETHYLAMINEmedicine.symptomMicrobiology (medical)medicine.medical_specialty610 Medicine & healthBiologyMETABOLISMlesion sizeMicrobiologyLesion03 medical and health sciencesINFLAMMATIONmedicine.arterymedicineAnimalsGerm-Free LifeHumanslcsh:RC799-869AddendumMice Inbred C57BLDisease Models Animal030104 developmental biologyReceptors LDLlow-density lipoprotein receptor-deficient mouseageLDL receptorlcsh:Diseases of the digestive system. Gastroenterologyatherosclerosisdescription
The microbiota has been linked to the development of atherosclerosis, but the functional impact of these resident bacteria on the lesion size and cellular composition of atherosclerotic plaques in the aorta has never been experimentally addressed with the germ-free low-density lipoprotein receptor-deficient (Ldlr(-/-)) mouse atherosclerosis model. Here, we report that 16 weeks of high-fat diet (HFD) feeding of hypercholesterolemicLdlr(-/-)mice at germ-free (GF) housing conditions did not impact relative aortic root plaque size, macrophage content, and necrotic core area. Likewise, we did not find changes in the relative aortic arch lesion size. However, late atherosclerotic GFLdlr(-/-)mice had altered inflammatory plasma protein markers and reduced smooth muscle cell content in their atherosclerotic root plaques relative to CONV-RLdlr(-/-)mice. Neither absolute nor relative aortic root or aortic arch plaque size correlated with age. Our analyses on GFLdlr(-/-)mice did not reveal a significant contribution of the microbiota in late aortic atherosclerosis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-11-01 |