Grape seed and skin extract reduces pancreas lipotoxicity, oxidative stress and inflammation in high fat diet fed rats.

6533b857fe1ef96bd12b4707

RESEARCH PRODUCT

Grape seed and skin extract reduces pancreas lipotoxicity, oxidative stress and inflammation in high fat diet fed rats.

Naim Akhtar Khan Kamel Charradi Ezzedine Aouani Faten Aloui Selvakumar Subramaniam Aziz Hichami Ferid Limam

subject

Male MESH : Oxidative Stress 0301 basic medicine MESH: Inflammation Antioxidant medicine.medical_treatment MESH: Grape Seed Extract medicine.disease_cause Antioxidants chemistry.chemical_compound 0302 clinical medicine MESH: Obesity Vitis MESH: Animals MESH : Pancreas 2. Zero hunger MESH: Oxidative Stress MESH : Grape Seed Extract MESH : Rats MESH : Diet High-Fat food and beverages General Medicine MESH: Pancreas 3. Good health Lipotoxicity 030220 oncology & carcinogenesis Seeds MESH : Antioxidants MESH : Obesity medicine.symptom medicine.medical_specialty Grape seed and skin extract MESH: Rats MESH : Male Inflammation Biology Diet High-Fat MESH : Rats Wistar MESH: Vitis MESH : Vitis 03 medical and health sciences Diabetes mellitus Internal medicine medicine Animals Obesity Rats Wistar Pancreas Pharmacology Inflammation MESH : Inflammation Grape Seed Extract Triglyceride Cholesterol MESH: Antioxidants [ SDV.SP.PHARMA ] Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology MESH: Rats Wistar medicine.disease MESH : Seeds MESH: Male Rats MESH: Diet High-Fat 030104 developmental biology Endocrinology chemistry MESH: Seeds Oxidative stress [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology MESH : Animals Steatosis Oxidative stress

description

IF 2.326; International audience; Obesity is related to an elevated risk of diabetes and the mechanisms whereby fat adversely affects the pancreas are poorly understood. We studied the effect of a high fat diet (HFD) on pancreas steatosis, oxidative stress and inflammation as well as the putative protection afforded by grape seed and skin extract (GSSE). HFD induced body weight gain, without affecting insulinemia, nor glycemia and dropped adiponectemia. HFD also provoked the ectopic deposition of cholesterol and triglyceride, and an oxidative stress characterized by increased lipoperoxidation and carbonylation, inhibition of antioxidant enzyme activities such as CAT, GPx and SOD, depletion of zinc and a concomitant increase in calcium and H2O2. HFD induced pro-inflammatory chemokines mRNA as RANTES and MCP1 as well as cytokines expression as TNFα, IL6 and IL1β. Importantly GSSE counteracted all the deleterious effects of HFD on pancreas in vivo i-e lipotoxicity, oxidative stress and inflammation. In conclusion, GSSE could find potential applications in fat-induced pancreas lipotoxicity and dysfunction.

year	journal	country	edition	language
2016-12-01

10.1016/j.biopha.2016.11.017 https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01452690