6533b85efe1ef96bd12c06a1
RESEARCH PRODUCT
Retinas of the Diurnal RodentArvicanthis ansorgeiAre Highly Resistant to Experimentally Induced Stress and Degeneration
Domitille L. BoudardNiyazi AcarLionel BrétillonDavid Hicks. Umr Inra / Univ. Bourgogne / Cnrs : Centre Des Sciences Du Goût Et De L'alimentationUpr3212 Institut Des Neurosciences Cellulaires Et Intégratives (Inci) Université De Strasbourg 1subject
MaleRetinal degenerationLightRodentsprague dawlayFatty Acids Nonesterifiedbright cyclic lightMicechemistry.chemical_compound0302 clinical medicine[SDV.IDA]Life Sciences [q-bio]/Food engineeringoxidative stressmethyl-N-nitrosoufrea0303 health sciencesbiologymedicine.diagnostic_testmouse retinaRetinal DegenerationMethylnitrosoureaAnatomydocosahexaenoic acidCircadian Rhythmmedicine.anatomical_structureDocosahexaenoic acidRetinal Cone Photoreceptor CellsN-3 fatty acidsPhototoxicityAlkylating Agentsmedicine.medical_specialtylight-induced degeneration03 medical and health sciencesSpecies SpecificityStress Physiologicalbiology.animalInternal medicineElectroretinographymedicineAnimals[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering030304 developmental biologyRetinaRetinalmedicine.diseaseMice Inbred C57BLMuridaeratsTissue DegenerationDisease Models AnimalEndocrinologyrhodopsinchemistryregenerationinduced photoreceptor apoptosis030221 ophthalmology & optometrysense organsElectroretinographydescription
International audience; PURPOSE. Environmentally induced stress plays a significant role in retinal degeneration and blindness both in animals and in humans. Among such sources of stress, phototoxicity is well studied and has been shown to lead to photoreceptor-specific loss in a number of species. However, the vast majority of studies have been conducted in nocturnal, albino rod-dominant rat and mouse strains, and the pertinence of such findings to human pathology and cone loss is debatable. The authors examined retinal vulnerability to damage in the diurnal murid rodent Arvicanthis ansorgei, a pigmented species with a large number of cones. METHODS. The authors used established protocols for exposing animals to a wide range of lighting conditions (variable intensity, duration, spectrum, previous light history, and time of exposure) and injecting N-methyl-N-nitrosourea (MNU); each procedure is reported to produce rapid and complete photoreceptor-specific damage. Animals then underwent electroretinography to record rod and cone function and were subsequently euthanized and used for immunohistochemical analysis of retinal structure and quantification of free fatty acids. RESULTS. These standard regimens produced no detectable detrimental effects on A. ansorgei retinal phenotype, function, or structure. Partial retinal damage in A. ansorgei was induced by very intense blue light or elevated doses of MNU. This resistance was not attributable to differences in lipid composition (specifically, docosahexaenoic acid) between A. ansorgei and susceptible strains of mice and rats. CONCLUSIONS. The retina of this species exhibits exceptionally high resistance to damage from light and toxins such as MNU. (Invest Ophthalmol Vis Sci. 2011;52:8686-8700) DOI: 10.1167/iovs.11-8162
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-01-01 | Investigative Opthalmology & Visual Science |