6533b861fe1ef96bd12c581b
RESEARCH PRODUCT
Effect of cobalt and silver nanoparticles and ions on Lumbricus rubellus health and on microbial community of earthworm faeces and soil
Fabio De PascalisVito Armando LaudicinaAntonietta GattiMichele NacucchiLivia Vittori AntisariLuigi BadaluccoSerena CarboneSara FerrandoChiara Gambardellasubject
Earthworm faeceMicroorganismSettore AGR/13 - Chimica AgrariaSoil Science010501 environmental sciences01 natural sciencesEarthworm faeces Microbial community structure Microbial respiration Earthworm health Phospholipid fatty acidsMicrobial respirationBotanyClitellum0105 earth and related environmental sciencesbiologyEcologyEarthwormEarthworm faecesMicrobial community structure04 agricultural and veterinary sciencesbiology.organism_classificationLumbricus rubellusManureAgricultural and Biological Sciences (miscellaneous)Microbial population biologyEnvironmental chemistryMicrobial community structure;Phospholipid fatty acids;Earthworm health;Earthworm faeces;Microbial respiration040103 agronomy & agriculturePhospholipid fatty acidPhospholipid fatty acids0401 agriculture forestry and fisheriesMicrocosmEarthworm healthBacteriadescription
The aim of this study was to investigate the impact of silver and cobalt, supplied both as ions and nanoparticles (Ag+, Co2+, AgNPs, CoNPs) through contaminated food to earthworms (Lumbricus rubellus), on their health as well as on microbial community of both soil and earthworm faeces. Earthworms and microbes were exposed to the contaminants in laboratory microcosms with artificial soil. Contaminants were supplied once a week for 5 weeks by spiking them on horse manure. The accumulation of CoNPs and Co2+ in earthworm tissues was two and three times greater than AgNPs and Ag+, respectively. Except for AgNPs, contaminants significantly affected microbial community structure of earthworm faeces by increasing G-bacteria, thus also increasing the bacteria fungi ratio while decreasing the G+/G- bacteria ratio. Such shift was also reflected on soil microbial community, thus suggesting a close relationship between microbial community of soil and of earthworm faeces. Neither of the Co treatments affected soil microbial basal respiration whereas they increased the microbial biomass specific respiration or metabolic quotient, suggesting some stress induction on soil microorganisms. Earthworm health was strongly affected as revealed by the reduced fluidity of fatty acids extracted from the body tissues. In addition, the histological investigations, after the depuration period, showed positive results about the NPs toxicity. In particular, TUNEL-positive nuclei in epidermis and in peritoneum, suggest the presence of toxicosis. The ESEM-EDS technique revealed the presence of Ca-P spherules (calcification) between mouth and clitellum of earthworms fed with Co2+ contaminated food. (C) 2016 Elsevier B.V. All tights reserved.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-12-01 |