Lysozyme gene expression and hemocyte behaviour in the Mediterranean mussel, Mytilus galloprovincialis, after injection of various bacteria or temperature stresses.

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RESEARCH PRODUCT

Lysozyme gene expression and hemocyte behaviour in the Mediterranean mussel, Mytilus galloprovincialis, after injection of various bacteria or temperature stresses.

Matteo Cammarata Hui Li Philippe Roch Maria Giovanna Parisi Maria-giovanna Parisi Mylène Toubiana

subject

Vibrio anguillarum Hemocytes Population Lysozyme Mytilu Vibrio splendidus Aquatic Science Gene Expression Regulation Enzymologic Microbiology Micrococcus 03 medical and health sciences chemistry.chemical_compound ISH Gene expression RNA Ribosomal 28S Environmental Chemistry Micrococcus lysodeikticus Animals Vibrio anguillarum education 030304 developmental biology Vibrio Mytilus 0303 health sciences Messenger RNA education.field_of_study Vibrio splendidu biology Q-PCR Muscles Temperature 04 agricultural and veterinary sciences General Medicine Molluscs biology.organism_classification Micrococcus lysodeikticu Mytilus Real-time polymerase chain reaction chemistry Heat shock Q PCR 040102 fisheries 0401 agriculture forestry and fisheries Muramidase Gene expression Lysozyme Bacteria

description

The aim of the present study was to evaluate the expression of the Mytilus galloprovincialis lysozyme gene in different in vivo stress situations, including injection of bacteria Vibrio splendidus LGP32, Vibrio anguillarum or Micrococcus lysodeikticus, as well as heat shock at 30 C and cold stress at 5 C. Injection of V. splendidus LGP32 resulted in: (i) a general down-regulation of lysozyme gene expression, as quantified by Q-PCR; (ii) reduction in the number of circulating hemocytes; (iii) decrease in the percentage of circulating hemocytes expressing lysozyme mRNA which was now restricted to only small cells, as observed by ISH; and (iv) accumulation of hemocytes expressing lysozyme in the muscle sinus where injection took place. Injection of V. anguillarum or M. lysodeikticus induced significant up-regulation of lysozyme gene expression, but only 2-3 days post-injection, with no change in the total hemocyte counts but an increased percentage of hemocytes expressing lysozyme mRNA. Neither the control injection of PBS-NaCl nor temperature stress modified the lysozyme expression pattern. Consequently, the hemocyte population appears to be capable of discriminating between stress factors, and even between 2 Vibrio species. (C) 2008 Elsevier Ltd. All rights reserved.

year	journal	country	edition	language
2008-07-01	Fishshellfish immunology

10.1016/j.fsi.2008.04.001 https://pubmed.ncbi.nlm.nih.gov/18495491