Histological and histomorphometrical analysis of a silica matrix embedded nanocrystalline hydroxyapatite bone substitute using the subcutaneous implantation model in Wistar rats.

6533b862fe1ef96bd12c62b1

RESEARCH PRODUCT

Histological and histomorphometrical analysis of a silica matrix embedded nanocrystalline hydroxyapatite bone substitute using the subcutaneous implantation model in Wistar rats.

Benjamin W Thimm Ines Willershausen Patrick Booms Shahram Ghanaati Stefan Stübinger Mike Barbeck Constantin A. Landes R.a. Sader Charles James Kirkpatrick Carina Orth

subject

Materials science Bone substitute Biomedical Engineering 2204 Biomedical Engineering Bioengineering 610 Medicine & health Biomaterials Subcutaneous Tissue In vivo Absorbable Implants Materials Testing Animals Rats Wistar Bone regeneration 11077 Center for Applied Biotechnology and Molecular Medicine 1502 Bioengineering 2502 Biomaterials Biomaterial Silicon Dioxide Nanocrystalline material Rats Drug Combinations Durapatite Giant cell Silica matrix Bone Substitutes Subcutaneous implantation Nanoparticles Female Biomedical engineering

description

The clinical suitability of a bone substitute material is determined by the ability to induce a tissue reaction specific to its composition. The aim of this in vivo study was to analyze the tissue reaction to a silica matrix-embedded, nanocrystalline hydroxyapatite bone substitute. The subcutaneous implantation model in Wistar rats was chosen to assess the effect of silica degradation on the vascularization of the biomaterial and its biodegradation within a time period of 6 months. Already at day 10 after implantation, histomorphometrical analysis showed that the vascularization of the implantation bed reached its peak value compared to all other time points. Both vessel density and vascularization significantly decreased until day 90 after implantation. In this time period, the bone substitute underwent a significant degradation initiated by TRAP-positive and TRAP-negative multinucleated giant cells together with macrophages and lymphocytes. Although no specific tissue reaction could be related to the described silica degradation, the biomaterial was close to being fully degraded without a severe inflammatory response. These characteristics are advantageous for bone regeneration and remodeling processes.

year	journal	country	edition	language
2010-05-11	Biomedical materials (Bristol, England)

10.1088/1748-6041/5/3/035005 https://pubmed.ncbi.nlm.nih.gov/20460687