6533b86dfe1ef96bd12c9771
RESEARCH PRODUCT
A role for peroxisome proliferator-activated receptor gamma in resveratrol-induced colon cancer cell apoptosis.
Dominique DelmasDominique DelmasEmeric LimagneEmeric LimagneAlessandra ScagliariniAlessandra ScagliariniVirginie AiresVirginie AiresAnnie CarlierBertrand BrassartEric SolaryLaurent MartinyStéphane MandardStéphane MandardMichel Tarpinsubject
endocrine system diseasesColorectal cancerPeroxisome proliferator-activated receptorApoptosisPharmacologyResveratrolresveratrolMESH: ThiazolidinedionesPPAR[ SDV.CAN ] Life Sciences [q-bio]/Cancerchemistry.chemical_compound0302 clinical medicineMESH: StilbenesStilbenesMESH : Cell Proliferation[ SHS.INFO ] Humanities and Social Sciences/Library and information sciencesAnilidesskin and connective tissue diseaseschemistry.chemical_classification0303 health sciencesfood and beveragesCell cycle3. Good healthMESH : ThiazolidinedionesMESH : Colonic Neoplasmscolon cancer030220 oncology & carcinogenesisColonic NeoplasmsS Phase Cell Cycle CheckpointsRosiglitazonehormones hormone substitutes and hormone antagonistsBiotechnologymedicine.drugMESH : PPAR gammaMESH: Cell Line Tumor[SHS.INFO]Humanities and Social Sciences/Library and information sciences[SDV.CAN]Life Sciences [q-bio]/Cancer[SDV.BC]Life Sciences [q-bio]/Cellular BiologyBiologyMESH: Anilides[SHS.INFO] Humanities and Social Sciences/Library and information sciencesMESH : AnilidesMESH : StilbenesRosiglitazone03 medical and health sciences[SDV.CAN] Life Sciences [q-bio]/CancerCell Line TumorMESH: Cell Proliferationmedicine[SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular BiologyHumans[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology[SDV.BC] Life Sciences [q-bio]/Cellular BiologyMESH : S Phase Cell Cycle Checkpoints[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular Biologypolyphenols030304 developmental biologyCell ProliferationMESH: Colonic NeoplasmsMESH: HumansCell growthMESH : Cell Line Tumor[ SDV.BC ] Life Sciences [q-bio]/Cellular Biologyorganic chemicalsMESH: ApoptosisMESH : Humansmedicine.diseasePPAR gammaMESH: S Phase Cell Cycle CheckpointschemistryMESH: PPAR gammaApoptosisCancer cellThiazolidinedionesMESH : ApoptosisFood Sciencedescription
Scope Resveratrol may function as a chemopreventive agent. A recent clinical study demonstrates a reduction in tumor cell proliferation in colorectal patients receiving repeated oral ingestion of resveratrol. However, gaps remain in our knowledge of the molecular mechanisms by which resveratrol exerts its chemopreventive effect. We have previously demonstrated that resveratrol induces apoptosis in colon cancer cells and that resveratrol can sensitize chemoresistant colon cancer cells to various drugs. Based on its ability to activate peroxisome proliferator-activated receptor gamma (PPARγ) in colon cancer cells, we sought to determine the implication of this nuclear transcription factor in resveratrol-induced apoptosis. Methods and results Transient transfection of cancer cells with a dominant-negative PPARγ mutant or treatment with a PPARγ antagonist (GW9662) reversed the inhibitory effect of resveratrol. Moreover, GW9662 prevented disruption of the cell cycle induced by resveratrol and consequently abrogated resveratrol-induced apoptosis. Tumor cell death was potentiated by combining resveratrol with rosiglitazone, a PPARγ agonist. Conclusion The results show that PPARγ plays a role in resveratrol-induced apoptosis of colon carcinoma cells. The combination of resveratrol with a PPARγ agonist could be a promising pharmacological approach for treatment of colorectal cancer.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-08-31 |