6533b86efe1ef96bd12cbf3c
RESEARCH PRODUCT
In-Gel Assay to Evaluate Antioxidant Enzyme Response to Silver Nitrate and Silver Nanoparticles in Marine Bivalve Tissues
Candida LorussoAntonio CalisiGianluca SaràFrancesco Donderosubject
Fluid Flow and Transfer ProcessesElectrophoresisSettore BIO/07 - Ecologiaelectrophoresis; <i>Mytilus galloprovincialis</i>; mussel; catalase; superoxide dismutase; hsp70; glutathione peroxidaseProcess Chemistry and TechnologyGeneral EngineeringSuperoxide dismutaseCatalaseComputer Science ApplicationsHsp70Mytilus galloprovincialisGlutathione peroxidaseGeneral Materials ScienceMusselInstrumentationdescription
Silver is back in vogue today as this metal is used in the form of nanomaterials in numerous commercial products. We have developed in-gel electrophoretic techniques to measure the activity of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPX), and used the same techniques in combination with HSP70 Western blot analysis to evaluate the effects of nanomolar amounts of silver nitrate and 5 nm alkane-coated silver nanoparticles in tissues of the marine bivalve Mytilus galloprovincialis (Lam.) exposed for 28 days in mesocosms. Our results showed a negligible effect for nanosilver exposure and dose-dependent effects for the nitrate form.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2022-03-08 |