Predicting shifting sustainability trade-offs in marine finfish aquaculture under climate change

Defining sustainability goals is a crucial but difficult task because it often involves the quantification of multiple interrelated and sometimes conflicting components. This complexity may be exacerbated by climate change, which will increase environmental vulnerability in aquaculture and potentially compromise the ability to meet the needs of a growing human population. Here, we developed an approach to inform sustainable aquaculture by quantifying spatio-temporal shifts in critical trade-offs between environmental costs and benefits using the time to reach the commercial size as a possible proxy of economic implications of aquaculture under climate change. Our results indicate that optim…

Functional spatial contextualisation of the effects of multiple stressors in marine bivalves

Abstract. Many recent studies have revealed that the majority of environmental stressors experienced by marine organisms (ocean acidification, global warming, hypoxia etc.) occur at the same time and place, and that their interaction may complexly affect a number of ecological processes. Here, we experimentally investigated the effects of pH and hypoxia on the functional and behavioural traits of the mussel Mytilus galloprovincialis, we then simulated the potential effects on growth and reproduction dynamics trough a Dynamic Energy Budget (DEB) model under a multiple stressor scenario. Our simulations showed that hypercapnia had a remarkable effect by reducing the maximal habitat size and r…

Polyculture as a tool to increase the economic income: a study case in the Gulf of Castellammare

Polyculture is a practice involving integration between organisms of different trophic levels (i.e. fish with low-trophic-level organism like molluscs and/or algae). This paper, reporting on a field experiment carried out during 2004 in the Gulf of Castellammare (South Tyrrhenian), showed that mussels transplanted in areas under fish farm influence grew up better than mussels transplanted far from fish farm. Thus, results pointed out polyculture as a reliable tool for i) increasing local bivalve production, ii) obtaining the reduction of importation from other countries and iii) increasing the environmental sustainability of fish aquaculture.

Growth of Mytilus galloprovincialis (mollusca, bivalvia) close to fish farms: a case of integrated multi-trophic aquaculture within the Tyrrhenian Sea

A current practice of marine aquaculture is to integrate fish with low-trophic-level organisms (e.g. molluscs and/or algae) during farming to minimise effects of cultivation on the surrounding environment and to potentially increase economic income. This hypothesis has been tested in the present article experimentally, by co-cultivating fish and mussels (Mytilus galloprovincialis) in the field. Integrated multi-trophic aquaculture (IMTA) experiments were started in July 2004 by transplanting mussel seed at two depths (-3 and -9 m) within 1,000 m downstream to fish cages and at 1,000 m upstream from cages. Mussels were cultured in nylon net bags for 12 months and the growth recorded biometri…

Heart beat rate adaptations to varying salinity of two intertidal Mediterranean bivalves: The invasiveBrachidontes pharaonisand the nativeMytilaster minimus

Abstract Changes in heart beat rates (HBR) of Brachidontes pharaonis (an invasive Lessepsian species) and Mytilaster minimus (a native Mediterranean species) in response to changes in salinity values from brackish (20) to extreme hyper-saline (75) were investigated both in situ and in the laboratory. The two species displayed different responses to varying salinity, with clear differences in HBR observed between groups investigated at different salinities and between groups originating from different environments. The native species, adapted to narrow salinity changes as those observed in the superficial waters of the Mediterranean Sea, showed signs of stress at salinities slightly above 37…

A mechanistic approach reveals non linear effects of climate warming on mussels throughout the Mediterranean sea

There is a dire need to forecast the ecological impacts of global climate change at scales relevant to policy and management. We used three interconnected models (climatic, biophysical and energetics) to estimate changes in growth, reproduction and mortality risk by 2050, for three commercially and ecologically important bivalves at 51 sites in the Mediterranean Sea. These results predict highly variable responses (both positive and negative) in the time to reproductive maturity and in the risk of lethality among species and sites that do not conform to simple latitudinal gradients, and which would be undetectable by methods focused only on lethal limits and/or range boundaries.

Moving Toward a Strategy for Addressing Climate Displacement of Marine Resources: A Proof-of-Concept

Realistic predictions of climate change effects on natural resources are central to adaptation policies that try to reduce these impacts. However, most current forecasting approaches do not incorporate species-specific, process-based biological information, which limits their ability to inform actionable strategies. Mechanistic approaches, incorporating quantitative information on functional traits, can potentially predict species- and population-specific responses that result from the cumulative impacts of small-scale processes acting at the organismal level, and can be used to infer population-level dynamics and inform natural resources management. Here we present a proof-of-concept study…

Effect of salinity and temperature on feeding physiology and scope for growth of an invasive species (Brachidontes pharaonis - MOLLUSCA: BIVALVIA) within the Mediterranean sea

Abstract The Indo-Pacific mytilid Brachidontes pharaonis (Bivalvia, Fischer 1870) offers an excellent model for the study of “Lessepsian migration” and the successive colonization at new Mediterranean locations. This species in out competing indigenous bivalves is particularly well adapted to Mediterranean conditions and this is likely due to biological characteristics and physio-ecological plasticity. In the present paper, we report on clearance rate (CR), respiration rate (RR) and Scope for Growth (SFG) of B. pharaonis collected from a Western Sicilian pond (Southern Tyrrhenian, MED). Physiological variables were determined in response to a range of temperatures from 11 ° to 20 °C and a b…

Relationships between Suspended and Sediment Organic Matter in a Semi-Enclosed Marine System: The Stagnone Di Marsala Sound (Western Sicily)

To gather information on the interactions between the sediment and suspended organic matter pools in the Stagnone di Marsala, water and sediment samples were collected, on a monthly basis, at 11 stations. Water temperature and salinity showed a clear seasonality whilst particulate and sediment organic matter did not show any clear seasonal pattern. Relative abundances of suspended and sediment organic matter, on the other hand, appeared to be site-dependent and controlled mainly by the dynamic balance between resuspension and sedimentation. High quantities of both suspended and sediment total organic matter were present, while very low algal biomasses (in terms of chlorophyll-a concentratio…

The role and contribution of the seagrass Posidonia oceanica (L.) Delile organic matter for secondary consumers as revealed by carbon and nitrogen stable isotope analysis

The δ13C and δ15N values of primary producers and consumers were studied to obtain information on the trophic role of Posidonia oceanica L. Delile, the dominant primary producer, in a Mediterranean shallow environment (the Stagnone di Marsala, western Sicily). δ13C strongly discriminated between pelagic and benthic pathways, with the former based on phytoplankton and the latter on a mixed pool of seagrass detritus, epiphytes and benthic algae as carbon sources. A particularly important trophic role appears to be performed by the vegetal epiphytic community on seagrass leaves (δ13C = -14.9 ± 0.1‰), which supports most of the faunal seagrass community (i.e. Amphipoda, Isopoda, Tanaidacea; δ13…

Silver Nanoparticles Affect Functional Bioenergetic Traits in the Invasive Red Sea Mussel Brachidontes pharaonis

We investigated the functional trait responses to 5 nm metallic silver nanoparticle (AgNPs) exposure in the Lessepsian-entry bivalveB. pharaonis. Respiration rate (oxygen consumption), heartbeat rate, and absorption efficiency were evaluated across an 8-day exposure period in mesocosmal conditions. Basal reference values from not-exposed specimens were statistically compared with those obtained from animals treated with three sublethal nanoparticle concentrations (2 μg L−1, 20 μg L−1, and 40 μg L−1). Our data showed statistically significant effects on the average respiration rate ofB. pharaonis. Moreover, complex nonlinear dynamics were observed as a function of the concentration level and…

Functional and energetic consequences of climate change on a predatory whelk

Abstract The increasing rise in sea surface temperature caused by human activities currently represents the major threat to biodiversity and natural food webs. In this study we used the Lessepsian mussel Brachidontes pharaonis, one of the most recent invaders of the Mediterranean Sea, as a model to investigate the effect of a novel prey and a chronic increase in temperatures on functional parameters of local consumers, compared to the native mytilid species Mytilaster minimus. In particular we focused on the whelk Stramonita haemastoma, a widespread Mediterranean intertidal predator that actively preys on bivalves, barnacles and limpets, by studying the direct effects of such multiple stres…

The impact of climate change on Mediterranean intertidal communities: losses in coastal ecosystem integrity and services

As has been shown for other ecosystems, the ecological and socio-economic impacts of climate change on Mediterranean intertidal habitats are highly variable in space and time. We conducted field and laboratory measurements of cellular, ecophysiological and behavioural responses of selected intertidal invertebrates (mussels, gastropods and sponges) and completed a literature review to determine what is known of socioeconomic consequences of these biological changes. Results suggest significant gaps in our knowledge that may impede a complete understanding of likely impacts (physical, biological, and socioeconomic) and that sufficient data for such an analysis is available only for mussels. A…

Particulate Organic Matter Composition in A Semi-Enclosed Marine System

Spatial and temporal changes in the biochemical composition of particulate organic matter in a semi-enclosed marine system (Stagnone di Marsala, Mediterranean Sea) were studied, on a monthly basis, from January to December 1994, in order to assess nutritional value of suspended particles for benthic suspension feeders. According to previous findings, the study site displayed a strong oligotrophy. Chlorophyll-a accounted for a very low fraction of the total suspended matter pool (0.1%), whereas at least 75% of POC was of detrital/heterotrophic origin. POC: PON ratio values indicate that bacterioplankton biomass accounted for a significant fraction of the total POC pool, displaying values com…

An improved noninvasive method for measuring heartbeat of intertidal animals

Since its emergence two decades ago, the use of infrared technology for noninvasively measuring the heartbeat rates of invertebrates has provided valuable insight into the physiology and ecology of intertidal organisms. During that time period, the hardware needed for this method has been adapted to currently available electronic components, making the original published description obsolete. This article reviews the history of heartbeat sensing technology, and describes the design and function of a modern and simplified infrared heartbeat rate sensing system compatible with many intertidal and marine invertebrates. This technique overcomes drawbacks and obstacles encountered with previous …

Meiofauna associated with vermetid reefs: the role of macroalgae in increasing habitat size and complexity

We present the first dataset of meiofauna associated with vermetid reefs (biogenic constructions of Mediterranean intertidal habitat) in two areas along the northern coast of Sicily, Italy. The vermetid reefs are characterized by a horizontal extension from the shore towards the open sea and can be divided into three zones (the inner margin, the cuvette zone and the outer margin) which differ in hydrodynamic features. We studied the spatial distribution of meiofauna along the horizontal axis of the vermetid reefs, investigating the communities inhabiting the sediment inside cuvettes (shallow pools inside the “cuvette zone”) located between the inner and the outer margins of the reefs. We ob…

Indoor spectroradiometric characterization of plastic litters commonly polluting the Mediterranean Sea: toward the application of multispectral imagery

AbstractAround 350 million tonnes of plastics are annually produced worldwide. A remarkable percentage of these products is dispersed in the environment, finally reaching and dispersed in the marine environment. Recent field surveys detected microplastics’ concentrations in the Mediterranean Sea. The most commonly polymers found were polyethylene, polypropylene and viscose, ethylene vinyl acetate and polystyrene. In general, the in-situ monitoring of microplastic pollution is difficult and time consuming. The main goals of this work were to spectrally characterize the most commonly polymers and to quantify their spectral separability that may allow to determine optimal band combinations for…

Characterization of mitotic chromosomes of four species of the genus Diplodus: Karyotypes and chromosomal nucleolar organizer region phenotypes

Karyotypes have been described in four Mediterranean species of the genus Diplodus (Teleostei, Sparidae), D. vulgaris, D. puntazzo, D. sargus and D. annularis. Chromosomes were mainly acrocentric in all but D. vulgaris, where certain chromosome pairs were subtelocentric. A remarkable intraspecific heteromorphism in the number of NOR-bearing chromosomes along with a substantial interspecific variability in position of chromosomal Ag signals have been encountered. The presumed origin of multiple NOR-bearing chromosomes in Diplodus species and variation of the NOR location is discussed. ? 1996 The Fisheries Society of the British Isles

Variations in physiological responses to thermal stress in congeneric limpets in the Mediterranean Sea

Cardiac activity (Arrhenius breakpoint temperatures and Q10 relationships) and heat shock response (hsp70 expression) were measured in the congeneric limpets Patella rustica, P. caerulea and P. ulyssiponensis in order to test the relationship between their vertical distribution and physiological thermal tolerance. These species exhibit different vertical distributions along Mediterranean shores and despite the narrow tidal range in the Mediterranean, they experience different environmental conditions and consequently had specific thermal windows. Cardiac activity of the upper zoned P. rustica was maintained at higher temperatures than its mid- or low shore counterparts, P. caerulea and P. u…

The effectiveness of fish feeding behaviour in mirroring trawling-induced patterns

The ability to observe and predict trawling-induced patterns at spatial and temporal scales that are relevant to inform realistic management strategies is a challenge which scientists have consistently faced in recent decades. Here, we use fish feeding behaviour, a biological trait easily impaired by trawling disturbance, to depict alterations in fish condition (i.e. individual fitness) and feeding opportunities. The benthivorous fish Mullus barbatus barbatus was selected as a model species. The observed trends of responses to trawling in prey species confirmed the effectiveness of a non-trawled zone in sustaining higher levels of diet diversity (e.g. quantity and quality of ingested prey) …

The effect of the quality of diet on the functional response of Mytilus galloprovincialis (Lamarck, 1819): Implications for integrated multitrophic aquaculture (IMTA) and marine spatial planning

Abstract The integrated multi-trophic aquaculture (i.e., IMTA) is a practice combining organisms with different trophic levels with the final purpose of transforming the continuous waste of food by targeting species into nutrient input for other non-target species. This practice very often involves filter feeders, such as bivalves, by the use of which bioenergetics budgets are strongly influenced by the quality and quantity of different foods. However, to date, scant information is available, to really understand the rebounds of food availability on the growth performances of these harvested biomasses in the natural environment. By choosing the mussel Mytilus galloprovincialis as a model, t…

Bright spots as climate‐smart marine spatial planning tools for conservation and blue growth

Marine spatial planning that addresses ocean climate-driven change (‘climate-smart MSP’) is a global aspiration to support economic growth, food security and ecosystem sustainability. Ocean climate change (‘CC’) modelling may become a key decision-support tool for MSP, but traditional modelling analysis and communication challenges prevent their broad uptake. We employed MSP-specific ocean climate modelling analyses to inform a real-life MSP process; addressing how nature conservation and fisheries could be adapted to CC. We found that the currently planned distribution of these activities may become unsustainable during the policy's implementation due to CC, leading to a shortfall in its s…

Cetacean presence and distribution in the central Mediterranean Sea and potential risks deriving from plastic pollution

Abstract The Sardinian and Sicilian Channels are considered hotspots of biodiversity and key ecological passages between Mediterranean sub-basins, but with significant knowledge gaps about marine mammal presence and potential threats they face. Using data collected between 2013 and 2019 along fixed transects, inter and intra-annual cetacean index of abundance was assessed. Habitat suitability, seasonal hot spots, and risk exposure for plastic were performed using the Kernel analysis and the Biomod2 R-package. 661 sightings of 8 cetacean species were recorded, with bottlenose and striped dolphins as the most sighted species. The north-eastern pelagic sector, the coastal waters and areas near…

Temporal and spatial patterns of trawl fishing activities in the Adriatic Sea (Central Mediterranean Sea, GSA17)

Abstract Trawl fishing activities have occurred for centuries on large spatial scale in the entire Mediterranean Sea, and today they are considered as one of the main and widespread causes of anthropogenic disturbance and habitat alteration in the marine environment. In order to delineate when, where and how marine ecosystems have been perturbed and to implement ecosystem-based management strategies, the identification and investigation of the spatial and temporal distribution of fishing effort and the fleet dynamics play a key role. In this context, Geospatial Technologies such as the Automatic Identification System (AIS) could represent a useful tool. The aim of the present work is to rec…

Predicting effective aquaculture in subtropical waters: A dynamic energy budget model for the green lipped mussel, Perna viridis

Abstract The green lipped mussel, Perna viridis, is an important aquaculture species throughout the Indo-Pacific region where production is often impacted by environmental degradation. To predict the impacts and mitigate against environmental problems due to various kinds of anthropogenic pollution, such as heavy metals and eutrophication, on P. viridis aquaculture a Dynamic Energy Budget (DEB) model was constructed. By integrating species-specific parameters and regional-specific environmental data the DEB model determined how the life history traits of P. viridis respond to changing environmental conditions. Using various levels of basal maintenance costs and food availability to elucidat…

Effects of fish farming waste to sedimentary and particulate organic matter in a southern Mediterranean area (Gulf of Castellammare, Sicily): a multiple stable isotope study (δ13C and δ15N)

Abstract Carbon and nitrogen stable isotope analysis was used to investigate the dispersion area of waste material coming from fish farming activities in the western Mediterranean. Tests were conducted to see if uneaten feed and faecal material isotopic signals, originating from fish farms, could be detected in particulate organic matter (POM) and sedimentary organic matter (SOM). The detectable dispersion distance (from under cages as far as 1000 m) of cage-derived organic material was also examined. To do this, carbon (δ13C) and nitrogen (δ15N) composition in POM and SOM collected around the cages, in some control areas and in the waste material, was measured. Mean POM δ13C was −22.9±0.2‰…

The author's reply to N.R. Haddaway.

Abstract In this reply we respond to the commentary of Dr. Haddaway addressed in searching for pitfalls in our systematic mapping exercise “Collating science-based evidence to inform public opinion on the environmental effects of marine drilling platforms in the Mediterranean Sea” recently published in Journal of Environmental Management (Mangano, M.C. and Sara, G. 2017. Journal of Environmental Management 188: 195–202). We discussed each so called “pitfalls” and, in our opinion, the main cornerstones of systematic map – SM (repeatability, comprehensiveness, transparency, traceability, quality, generalizability) are safe guaranteeing the “gold standard” required by this technique. Where nee…

and variability in Posidonia oceanica associated with seasonality and plant fraction

Abstract The carbon and nitrogen isotopic compositions of fractions of the seagrass Posidonia oceanica (L.) Delile in a Mediterranean shallow environment (Stagnone di Marsala, western Sicily) were investigated seasonally throughout 1998. The stable isotope ratios of seagrass leaves (intermediate and adult), rhizomes, leaf litter and aegagropiles were compared over 1 year in order to distinguish between seasonal and plant part variability. Significant differences in the isotopic composition tested using ANOVA were observed as a function of both plant fraction and season. There was an overall trend towards less discrimination against 13 C in summer (average δ 13 C ∼ −10‰) than in winter (aver…

Multi-level responses of Mytilus galloprovincialis to environmental stressors

Ocean acidification and elevated temperature negatively affect recruitment, oxygen consumption and calcification of the reef-building Dendropoma cristatum early life stages: Evidence from a manipulative field study

Expected temperature rise and seawater pH decrease may affect marine organism fitness. By a transplant experiment involving air-temperature manipulation along a natural CO2 gradient, we investigated the effects of high pCO(2) (similar to 1100 mu atm) and elevated temperature (up to +2 degrees C than ambient conditions) on the reproductive success, recruitment, growth, shell chemical composition and oxygen consumption of the early life stages of the intertidal reef-building vermetid Dendropoma cristatum. Reproductive success was predominantly affected by temperature increase, with encapsulated embryos exhibiting higher survival in control than elevated temperature conditions, which were in t…

Multiple stressors facilitate the spread of a non-indigenous bivalve in the Mediterranean Sea

Aim The introduction of non‐indigenous species (NIS) via man‐made corridors connecting previously disparate oceanic regions is increasing globally. However, the environmental and anthropogenic factors facilitating invasion dynamics and their interactions are still largely unknown. This study compiles and inputs available data for the NIS bivalve Brachidontes pharaonis across the invaded biogeographic range in the Mediterranean basin into a species distribution model to predict future spread under a range of marine scenarios. Location Mediterranean Sea. Methods A systematic review produced the largest presence database ever assembled to inform the selection of biological, chemical and physic…

Combining heat-transfer and energy budget models to predict thermal stress in Mediterranean intertidal mussels

Recent studies have emphasised that organisms can experience physiological stress well within their geographic range limits. Developing methods for mechanistically predicting the presence, absence and physiological performance of organisms is therefore important because of the ongoing effects of climate change. In this study, we merged a biophysical–ecological (BE) model that estimates the aquatic (high tide) and aerial (low tide) body temperatures of Mytilus galloprovincialis with a Dynamic Energy Budget (DEB) model to predict growth, reproduction and mortality of this Mediterranean mussel in both intertidal and subtidal environments. Using weather and chlorophyll-a data from three Mediter…

Predictive mechanistic bioenergetics to model habitat suitability of shellfish culture in coastal lakes

Quantitative tools based on mechanistic modelling of functional traits able to enhance the sustainability of aquaculture and most other human activities (i.e. reducing the likelihood of detrimental impacts optimising productions), are especially important factors in the decision to site aquaculture facilities in coastal lakes, ponds and lagoons and, in the case of detrimental impact, to adopt mitigation measures. We tested the ability of mechanistic functional trait based models to predict life history traits of cultivable shellfish in shallow coastal lakes. Dynamic Energy Budget (DEB) models were run to generate spatially explicit predictions of Mytilus galloprovincialis life history (LH) …

Impact of COVID-19 on aquaculture sector in Malaysia: Findings from the first national survey

Abstract Malaysian aquaculture sector consists of 391,000 t of cultivated organisms produced per year with an economic value for over USD 700 million as estimated in 2019. The COVID-19 pandemic caused a large global human pandemic and it is currently ongoing, affecting profoundly Malaysian aquaculture, which is vital for seafood provision and security assurance. Thus, to increase our understanding about how the pandemic affects aquaculture sectors is crucial to design possible management measures both at local and national level. To do it, we designed a survey, starting in May 2020, consisting of 25 online questions to collect information about the impact of COVID-19 on the aquaculture sect…

Low temperature trumps high food availability to determine the distribution of intertidal mussels Perna perna in South Africa

Explanations of species distributions often assume that the absence of a species is due to its inability to tolerate an environmental variable. Recent modelling techniques based on the dynamic energy budget (DEB) theory offer an effective way of identifying how interacting environmental parameters influence distributions through non-lethal effects on growth and development. The mussel Perna perna is an abundant ecosystem engineer around the coasts of Africa, South America and the Arabian peninsula, with an unexplained 1500 km lacuna in its distribution on the west coast of South Africa. We used a DEB approach to explain its distribution in southern Africa and test the hypothesis that this l…

Conceptualizing ecosystem tipping points within a physiological framework

Connecting the nonlinear and often counterintuitive physiological effects of multiple environmental drivers to the emergent impacts on ecosystems is a fundamental challenge. Unfortunately, the disconnect between the way “stressors” (e.g., warming) is considered in organismal (physiological) and ecological (community) contexts continues to hamper progress. Environmental drivers typically elicit biphasic physiological responses, where performance declines at levels above and below some optimum. It is also well understood that species exhibit highly variable response surfaces to these changes so that the optimum level of any environmental driver can vary among interacting species. Thus, specie…

Seasonal patterns of biodiversity in Mediterranean coastal lagoons

Aim: Understanding and quantifying the seasonal patterns in biodiversity of phyto- benthos, macro-zoobenthos and fishes in Mediterranean coastal lagoons, and the species dependence upon environmental factors. Location: The study was carried out in the “Stagnone di Marsala e Saline di Trapani e Paceco,” the largest coastal lagoon system in the central Mediterranean Sea (Sicily, Italy), a Special Protection Area located along one of the central ecological corridors joining Africa and Europe. Methods: The coastal lagoon system was selected as a model ecosystem to investi- gate the seasonal variations in biodiversity indices and dominance–diversity relation- ships in phytobenthos, macro-zoobent…

The invasive blue crab Callinectes sapidus thermal response: Predicting metabolic suitability maps under future warming Mediterranean scenarios

One of the consequences of climate change and globalization is the recent proliferation of the invasive blue crab Callinectes sapidus in the Mediterranean Sea. In this study, C. sapidus thermal tolerance was investigated through experiments based on species metabolic response (measuring respiration rates) to a wide temperature range. Based on metabolic rates, Thermal Habitat Suitability (THS) maps were performed on current and futures temperature conditions in the Mediterranean Sea. Thermal Performance Curve showed a CTmax at 40°C and an optimum at 24°C. Respiration rate increased between 12°C and 24°C and decreased until 30°C. At the highest temperatures (> to 30°C) a pointed increa…

MULTIPLE CLIMATE-DRIVEN CASCADING ECOSYSTEM EFFECTS AFTER THE LOSS OF A FOUNDATION SPECIES

Abstract Climate change is evolving so fast that the related adverse effects on the environment are becoming noticeable. Thus, there is an urgent need to explore and understand the effects generated by multiple extreme climatic events (MECEs) on marine ecosystem functioning and the services provided. Accordingly, we combined long-term in-situ empirical observations in the Mediterranean Sea with a mesocosm manipulation to investigate the concurrence of increasing temperature and hypoxia events. By focussing on a foundation mussel species, we were able to detect several cascade events triggered by a mass mortality event caused by stressful temperature and oxygen conditions, and resulting in a…

Filtration pressure by bivalves affects the trophic conditions in Mediterranean shallow ecosystems

Bivalve filtration may control the amount of seston in coastal waters, reducing local euthrophication and keeping degrading phenomena like hypoxia and anthropogenic pollution under control. Two Sicilian brackish-marine ponds (Ganzirri and Faro) present us with the opportunity to gain data on the effect of bivalve filtration on the amount of particulate organic matter in the field. The cultivation of bivalves has been carried out in both of the ponds since the early 1990s but stopped in Ganzirri in 1995.We tested whether the cessation of bivalve cultivation influenced features of organic matter available to suspension feeders (total suspended matter, its inorganic and organic fractions, chlo…

Behavioural strategy of common bottlenose dolphins (Tursiops truncatus) in response to different kinds of boats in the waters of Lampedusa Island (Italy)

Owing to the increase of boat-traffic in the ocean many studies have been conducted to determine the response of bottlenose dolphin (Tursiops truncatus) to this kind of disturbance. This species is affected by boats in various ways and the response depends on the behavioural state of the dolphin but also on the kind of vessel. This study aimed to determine the effect of motorboats and trawlers on dolphins' presence, permanence in the area and whistle parameters in Lampedusa waters (Italy). Sampling was carried out between May and December 2006 and between July and September 2009, using experimental passive acoustic monitoring systems (PAM); a total of 300 h of recordings and 3000 whistles w…

Effects of microplastics on the functional traits of aquatic benthic organisms: A global-scale meta-analysis

Microplastics are widespread in the aquatic environment and thus available for many organisms at different trophic levels. Many scientific papers focus their attention on the study of the effects of microplastics on different species at individual level. Here we performed a global scale meta-analysis focusing our work on the study of the effect of microplastics on the functional traits of aquatic benthic organisms. Overall, microplastics showed a moderate negative effect on the examined functional traits of benthic organisms. Our results show that some crucial functional traits, such as those linked to behaviour and feeding, appear to be unaffected by microplastics. In contrast, traits rela…

Experiences of integrated mariculture in a southern Tyrrhenian area (Mediterranean Sea)

To ascertain the potential for exploiting marine areas for mariculture, data on the cultivation of molluscs and fish in the open sea of the southern Tyrrhenian were collected from May 1994 to June 1995. The aims of this integrated study were to test simple breeding methods for molluscs and fish, to apply these to the practices employed by local fishermen and to experiment with the use of a cage system requiring a low level of investment. Crassostrea gigas (Thunberg) and Mytilus galloprovincialis (Lamarck) were cultivated on submerged long lines around cages used for cultivating Seriola dumerili (Risso) and Diplodus puntazzo (Cetti). S. dumerili specimens were placed in two cages and fed wit…

Impact on the water column biogeochemistry of a Mediterranean mussel and fish farm

We investigated and compared the impact of organic loads due to the biodeposition of mussel and fish farms on the water column of a coastal area of the Tyrrhenian Sea (Western Mediterranean). Physico-chemical data (including oxygen, nutrients, DOC and particulate organic matter), microbial variables (picoplankton and picophytoplankton density and biomass) and phytoplankton biomass (as chlorophyll-a) were determined on a monthly basis from March 1997 to February 1998. The results of this study indicate that both fish farm and mussel culture did not alter significantly dissolved inorganic phosphorus and chlorophyll-a values, while inorganic nitrogen concentrations were higher in mussel farm a…

Noise elicits hematological stress parameters in Mediterranean damselfish (Chromis chromis, perciformes): A mesocosm study

In the last few decades, technological developments and the widespread rise of anthropic activities have increased the exposure of organisms to noise pollution, thus evoking great interest in its biological effects, particularly on the immune system. The aim of the present work was to investigate some of the biochemical parameters in the blood of Chromis chromis (Linnaeus, 1758) following in vivo exposure to noise levels of 200 and 300 Hz. Our results revealed that, compared to the control specimens, the fish exposed to noise had significantly increased levels of stress biomarkers such as glucose, lactate and total proteins in plasma, as well as a rise in the expression of heat shock protei…

Modeling Macroalgal Forest Distribution at Mediterranean Scale: Present Status, Drivers of Changes and Insights for Conservation and Management

Macroalgal forests are one of the most productive and valuable marine ecosystems, but yet strongly exposed to fragmentation and loss. Detailed large-scale information on their distribution is largely lacking, hindering conservation initiatives. In this study, a systematic effort to combine spatial data on Cystoseira C. Agardh canopies (Fucales, Phaeophyta) was carried out to develop a Habitat Suitability Model (HSM) at Mediterranean scale, providing critical tools to improve site prioritization for their management, restoration and protection. A georeferenced database on the occurrence of 20 Cystoseira species was produced collecting all the available information from published and grey lit…

The trophic transfer of persistent pollutants (HCB, DDTs, PCBs) within polar marine food webs.

Biomagnification (increase in contaminant concentrations at successively higher levels of trophic web), is a process that can transversally impair biodiversity and human health. Most research shows that biomagnification should be higher at poles with northern sites having a major tendency to biomagnify Persistent Organic Pollutants (POPs) through their marine food webs. We investigated the biomagnification degree into two marine trophic webs combining carbon and nitrogen stable isotopes and POP analyses. We showed that the Antarctic trophic web was more depleted than the sub-Arctic one and the differences highlighted for the basal part could explain the difference in length between them. Co…

Status of vulnerable Cystoseira populations along the Italian infralittoral fringe, and relationships with environmental and anthropogenic variables

We analyzed the occurrence and status of infralittoral fringe populations of Cystoseira spp. (Fucales) at thirteen rocky sites around the Italian coastline, and explored the relationships with relevant environmental and anthropogenic variables. We found Cystoseira populations at 11 sites: most were scattered and comprised monospecific stands of C. compressa, and only 6 sites also supported sparse specimens of either C. amentacea var. stricta or C. brachycarpa. Coastal human population density, Chlorophyll a seawater concentrations, sea surface temperature, annual range of sea surface temperature and wave fetch explained most of the variation of the status of C. compressa. We hypothesize a g…

The comparative biological effects of spatial management measures in protecting marine biodiversity: a systematic review protocol

Background: The global decline of marine biodiversity and the perceived need to protect marine ecosystems from irreparable alterations to ecosystem functioning and ecosystem service provision have produced an extensive range of spatial management measures (SMMs). The design of SMMs is a complex process often involving the integration of both conservation objectives and socio-economic priorities and the resultant trade-offs are highly dependent on the management regime in place. Future marine management is likely to involve greater use of different forms of protected areas with differing levels of protection, particularly for sites where there are multiple competing demands. Consequently, ev…

Mapping the potential for offshore aquaculture of salmonids in the Yellow Sea

AbstractMariculture has been one of the fastest-growing global food production sectors over the past three decades. With the congestion of space and deterioration of the environment in coastal regions, offshore aquaculture has gained increasing attention. Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) are two important aquaculture species and contribute to 6.1% of world aquaculture production of finfish. In the present study, we established species distribution models (SDMs) to identify the potential areas for offshore aquaculture of these two cold-water fish species considering the mesoscale spatio-temporal thermal heterogeneity of the Yellow Sea. The values of the a…

Functional trait-based layers - an aquaculture siting tool for the Mediterranean Sea

Abstract Aquaculture, the current fastest-growing food sector, is one of the major opportunities that could be reaped to cope with the increased demand for proteins from the sea and simultaneously generate economic growth while ensuring sustainable use of natural resources. The number of tools and approaches suggested to promote the selection of suitable areas - focusing mostly on the management of potential conflicting uses at sea - is rapidly increasing. However, to date, there is a lack of information regarding spatial planning according to a trait-based approach encompassing the functional and biological data of farmed species; a gap that may lead to selecting unsuitable areas for farmi…

Effects of fish-farm biodeposition on periphyton assemblages on artificial substrates in the southern Tyrrhenian Sea (Gulf of Castellammare, Sicily)

An algal assemblage growing on artificial substrata of fish-farm cages was investigated. Specifically, algal response to the effects of fish-farm facilities was studied, in order to identify a possible future descriptor of biodeposition impact. Some sites were positioned upstream of the farms (at least 750 m; ‘controls’) and other sites were positioned downstream of the farms (‘impacts’). All sites were situated in the Tyrrhenian Sea. Control and impact sites differed significantly with regard to the dissolved nutrient profile. The fouling community (samples were scraped from buoys) displayed a reduction gradient in diversity which increased with the effect of fish farms. A total of 51 taxa…

The Mediterranean intertidal habitat as a natural laboratory to study climate change drivers of geographic patterns in marine biodiversity.

Taking the acid test: Mediterranean limpets face up to climate change

Impacts of climate change involve the interactions of multiple stressors on intertidal organisms, but rarely are the impacts of these stressors examined together. Many tests are also conducted in artificial, controlled laboratory conditions, without making use of natural opportunities to test performance of organisms under different environmental stressors. Given its small tidal range, the Mediterranean Sea provides such an opportunity, with a very fine scale environmental gradient and species living very close to each other over the tidal gradient. The vertical distribution of the limpets, Patella rustica and P. caerulea overlap in Palermo, Sicily, but despite this they have different ther…

Hydrodynamic effects on the origin and quality of organic matter for bivalves: an integrated isotopic, biochemical and transplant study

Different hydrodynamic conditions can affect both the origin and the quality of organic matter available to bivalve molluscs. I chose 2 environments with very different hydrodynamics (a Mediterranean lagoon open to flow and a closed pond), but similar with regard to temperature, salinity, depth, wind exposure and algae coverage, to investigate this. The lagoon was characterised by active flow and bivalve molluscs, whereas the pond was closed off from the lagoon’s main flow but characterised by mussel beds of the highest density ever observed in the western Mediterranean. Biochemical features, 13C and 15N contents of particulate and sedimentary organic matter, and isotopic signatures of domi…

Parameterisation of bivalve functional traits for mechanistic eco-physiological dynamic energy budget (DEB) models

Mechanistic models such as those based on dynamic energy budget (DEB) theory are emergent ecomechanics tools to investigate the extent of fitness in organisms through changes in life history traits as explained by bioenergetic principles. The rapid growth in interest around this approach originates from the mechanistic characteristics of DEB, which are based on a number of rules dictating the use of mass and energy flow through organisms. One apparent bottleneck in DEB applications comes from the estimations of DEB parameters which are based on mathematical and statistical methods (covariation method). The parameterisation process begins with the knowledge of some functional traits of a tar…

The Effect of Temporal Changes and Environmental Trophic Condition on the Isotopic Composition (omega13C and omega15N) of Atherina boyeri (Risso, 1810) and Gobius niger (L., 1758) in a Mediterranean Coastal Lagoon (Lake of Sabaudia): Implications for Food Web Structure

δ13C and δ15N of organic matter sources and consumers were employed to analyse trophic differentiation between a benthic consumer, Gobius niger (L., 1758) (Pisces, Osteichthyes), and a pelagic consumer, Atherina boyeri (Risso, 1810) (Pisces, Osteichthyes) in a Mediterranean coastal lagoon (Lake of Sabaudia) in winter and summer 1999. Trophic differences between the two species throughout the two sampling periods were related to the environmental trophic condition (i. e. nutrient and phytopigment concentrations). Although these two fish have different habitats, they both exploited benthic organisms, above all in summer. When the nutrient and phytopigment concentrations were higher (summer), …

Monitoring the habitat use of common Bottlenose Dolphins (Tursiops truncatus) using passive acoustics in a Mediterranean marine protected area

The Mediterranean Tursiops truncatus subpopulation has been classified as Vulnerable on the IUCN Red List because of its decline. This species in coastal areas is exposed to a wide variety of threats: directed kills, bycatch, reduced prey availability caused by environmental degradation and overfishing, habitat degradation including disturbances from boat traffic and noise. Despite the increase in boat traffic in the Mediterranean Sea, the effect on T. truncatus’ habitat use has been studied in little detail and few data have been published. This study represents the first attempt to characterise spatial and temporal habitat use by T. truncatus and its relation to boat traffic in the Isole …

The aquaculture supply chain in the time of covid-19 pandemic: Vulnerability, resilience, solutions and priorities at the global scale

13 pages, 3 tables, 5 figures

Life history traits to predict biogeographic species distributions in bivalves

Organismal fecundity (F) and its relationship with body size (BS) are key factors in predicting species distribution under current and future scenarios of global change. A functional trait-based dynamic energy budget (FT-DEB) is proposed as a mechanistic approach to predict the variation of F and BS as function of environmental correlates using two marine bivalves as model species (Mytilus galloprovincialis and Brachidontes pharaonis). Validation proof of model skill (i.e., degree of correspondence between model predictions and field observations) and stationarity (i.e., ability of a model generated from data collected at one place/time to predict processes at another place/time) was provid…

Effects of trophic and environmental conditions on the growth of Crassostrea gigas in culture

In order to study the possibility of exploiting protected marine areas, comparative data on the cultivation of the oyster Cassostrea gigas in the South Tyrrenian Sea are reported. The oysters were cultured at -7 and - 13 m on long lines linked to artificial reefs. The observations, made during a 12-month period, were of the chemical-physical and trophic properties of the water column and growth rates of the oysters. Temperature ranged between 19.81 ± 4.67°C at -7 m and 18.03 ± 3.03°C at - 13 m. Salinity showed typical Mediterranean values. The area presented oligotrophic features: the chlorophyll-a (CHLa) concentration ranged between 0.05 ± 0.01 and 0.04 ± 0.02 μg 1-1 at -7 and -13 m, respe…

Evaluating fish assemblages associated with gas platforms: Evidence from a visual census technique and experimental fishing surveys

Fish assemblages associated with extractive platforms were surveyed, for the first time, using traditional fishing surveys (bottom gill net) and an underwater visual census (UVC) technique in order to test the effectiveness and to identify strengths and weaknesses of both methods. The study was carried out during three seasons at two offshore gas platforms (Eleonora and Squalo C) located in the central Adriatic Sea. Both methods recorded a similar number of fish species although with only eight species in common, thus supplying complementary information and a good estimate of the total fish species richness (39) associated with these gas platforms. The use of innovative techniques, such as …

Functional role of biofouling linked to aquaculture facilities in Mediterranean enclosed locations

Biofouling is generally considered a serious threat for human coastal activities such as aquaculture, and the ecological role of fouling organisms associated with fish-farm cages remains one of the most debated topics in the ecological field. However, although biofouling may cause significant problems related to human health, environmental impact and financial losses, in the past decade there has been an increasing interest in developing methods to promote the growth of biofouling on artificial structures as a strategy to mitigate human impacts and reduce the organic enrichment caused by net-cage fish farming. Here we investigated the filtration activity of biofouling assemblages colonizing…

The Use of Carbon Stable Isotopes to Investigate the Origin and Distribution of Suspended and Sedimentary Organic Matter in a Semi-enclosed Mediterranean Marine System

The natural stable isotope values of different primary sources have been used to trace the fate of organic carbon that enters in the marine food webs of Stagnone di Marsala (Italy). Water and sediment samples were collected monthly (March 1996 – February 1997) at 3 stations, characterized by different amounts of vegetal cover and analysed to determine total organic matter (OM), phytopigments, biopolymeric organic carbon (BPC) and stable carbon isotopic composition (δ13C), the latter measured also in main primary producers. Sedimentary OM accumulated in summer, while total suspended organic matter reached highest concentrations in May and December. The concentration of chlorophyll-a carbon i…

Influence of ambient temperature on the photosynthetic activity and phenolic content of the intertidal Cystoseira compressa along the Italian coastline

Understanding the physiological responses of intertidal seaweeds to environmental factors is fundamental to characterize their local physiological adaptation and success in the face of climate change. We measured the photosynthetic activity and the total phenolic content of the intertidal alga Cystoseira compressa and explored their relationship with latitude or local ambient air and seawater temperatures. Our results show that, when submerged, the photosynthetic activity of C. compressa showed values typical for non-stressed thalli, and the seawater temperatures found across sites explained the variability of these values. We observed a decrease in the photosynthetic activity of C. compres…

Assessing persistent organic pollutants (POPS) in the sicily island atmosphere, mediterranean, using PUF disk passive air samplers

In this study, PUF disk passive air samplers were deployed at eight sites, during two sampling periods, on the Island of Sicily in the Mediterranean basin. Samples were screened for a number of persistent organic pollutants (POPs) including polychlorinated biphenyls (PCBs) (n = 28 congeners), organochlorine pesticides (OCPs) (n = 16 compounds), and polybrominated biphenyl ethers (PBDEs n = 28) using GC-MS. PCB concentrations in air ranged ~10-300 pg m-3. The PCB pattern was dominated by lower to middle molecular weight PCBs (Cl3-5) and PCB-28 and PCB-52 were the most abundant congeners. α- and γ-Hexachlorocyclohexanes (HCHs) concentrations in air were relatively high ~420 ± 320 (50-1000) an…

Sedimentary and particulate organic matter: mixed sources for cockleCerastoderma glaucumin a shallow pond, Western Mediterranean

Seasonal changes in feeding habits and diet of the cockle Cerastoderma glaucum (Mollusca, Bivalvia) were analysed using carbon (δ 13 C) and nitrogen (δ 15 N) stable isotopes. I aimed to investigate the role of benthic and pelagic sources in the diet of this dominant infaunal bivalve on a western Mediterranean sandy bottomed pond. Adult C. glaucum and all potential organic sources (particulate and sedimentary organic matter, seagrass, macroalgae, het- erotrophic detritus) were collected and analysed for δ 13 Ca ndδ 15 N. In total 5 dominant organic sources were found, ranging between -21.0 and -8.0%� for δ 13 C and from 3.0 to about 7.0%� for δ 15 N. C. glaucum assimilated fraction ranged be…

Neglected fishery data sources as indicators of pre‐industrial ecological properties of Mediterranean swordfish ( Xiphias gladius , Xiphiidae)

Management of fish populations and ecosystems suffers from data and knowledge gaps, particularly with respect to how humans and nature affect dynamics at multi-decadal and longer time scales. However, collection of new data which indicates population or ecosystem status is slow and expensive. Here we analyse c. 110 years of neglected fishery data for an overexploited top predator, swordfish, in the Mediterranean Sea. These data are available at scales of high time–space–biological resolution (i.e., sub-weekly, sub-regional sea; individual weights) and allow different ecological questions to be addressed than is possible with coarsely scaled data (e.g., annually resolved total catches aggreg…

Sicilian transitional waters: Current status and future development

To appraise the current knowledge of Sicilian transitional waters (TWs), a review was undertaken of the information available on these ecosystems. In detail, a synthesis of the current status is reported, highlighting for each area the ecological features and status, historical data, conservation regime, environmental emergencies and anthropic pressures to which they are subject. The Sicilian TWs reviewed include coastal ponds and lakes, mires and areas with active and nonactive saltworks. Almost all of these ecosystems are affected by several protection regimes because of their high naturalistic value, although current knowledge is limited and fragmented. A few areas have received more att…

Enzymatically hydrolyzable protein and carbohydrate sedimentary pools as indicators of the trophic state of detritus sink system: a case study in a Mediterranean Coastal lagoon

In order to classify the trophic state of 'detritus sink' systems, instead of the conventional indicators based on inorganic nutrient availability and algal biomass and productivity in the water column, we utilized new biochemical descriptors based on the amount of sedimentary organic carbon and nitrogen potentially available to heterotrophic nutrition. We investigated spatial and temporal changes in microphytobenthic biomass, organic matter biochemical composition and enzymatically hydrolyzable protein and carbohydrate pools along a N-S transect in the Marsala lagoon (Mediterranean Sea, Italy) at three stations characterized by different hydrodynamic conditions and organic matter content i…

A meta-analysis on the ecological effects of aquaculture on the water column: dissolved nutrients

Environmental effects of aquaculture loadings have often been reviewed descriptively, and thus have not provided quantitative estimates of the overall response in the water column. Meta-analytical reviewing techniques allow the contextualisation of quantitative effects in the domain of current literature. In the present paper, more than 50 peer-reviewed articles were analysed and about 425 study cases used to test whether worldwide cultivations have a differential effect on dissolved nutrient levels. Meta-analysis feasibility depends on obtaining an estimate of the effect size from every study and the most common measure of effect size (Hedges’ d) is the difference between means of controls…

HCB, p,p'-DDE and PCB ontogenetic transfer and magnification in bluefin tuna (Thunnus thynnus) from the Mediterranean Sea.

The bluefin tuna, Thunnus thynnus (Linnaeus 1758), is biologically and economically important in the Atlantic--Mediterranean ecosystems. Bluefin tuna feed on diverse food items depending on their age, thus they occupy different trophic levels during their lifespan. Hexachlorobenzene (HCB), p,p'-DDE and polychlorinated biphenyls (PCBs) are well-known persistent organic pollutants (POPs) in the Mediterranean basin. The relationship between stable isotopes of nitrogen (N) and the POP residue levels in tissues has recently increased knowledge on the link between the trophic levels and the contaminant accumulation. Trophic levels were estimated by using 15N/14N ratio (delta15N) and HCB, p,p'-DDE…

Sources of carbon and dietary habits of new Lessepsian entry Brachidontes pharaonis (Bivalvia, Mytilidae) in the western Mediterranean

The sources of carbon and the dietary habits of Brachidontes pharaonis (Mollusca, Bivalvia), a new Lessepsian entry in the western Mediterranean, living in a cooling vat of a saltworks system in western Sicily (MED), were assessed by estimating throughout a season the relative abundance of a stable carbon isotope (δ13C) in particulate organic matter (POM), sedimentary organic matter (SOM), primary organic matter sources (seagrasses, sand microflora, macroalgae), Brachidontes pharaonis and its biodeposition material. In the saltworks the most enriched primary food source potentially fuelling the saltworks food web was Cymodocea nodosa (seasonal average -7.9±0.6‰), Laurencia papillosa and Cys…

Predicting biological invasions in marine habitats through eco-physiological mechanistic models: a case study with the bivalveBrachidontes pharaonis

Aim We used a coupled biophysical ecology (BE)-physiological mechanistic modelling approach based on the Dynamic Energy Budget theory (DEB, Dynamic energy budget theory for metabolic organisation, 2010, Cambridge University Press, Cambridge; DEB) to generate spatially explicit predictions of physiological performance (maximal size and reproductive output) for the invasive mussel, Brachidontes pharaonis. Location We examined 26 sites throughout the central Mediterranean Sea. Methods We ran models under subtidal and intertidal conditions; hourly weather and water temperature data were obtained from the Italian Buoy Network, and monthly CHL-a data were obtained from satellite imagery. Results …

Role of peat organic matter on isotopic composition of most abundant benthic organisms in intertidal habitats of SW Iceland

The transfer of peat organic matter (OM) from tidal pools of upper littoral to downstream rocky shores and its potential incorporation into marine biota were investigated using the stable isotope approach. Samplings were carried out in September 2004 in two SW Icelandic sites (Osar and Hvassahraun), where we selected (1) areas with shores where grass gently declined towards rocks and, on reaching the sea line, formed small tidal peat pools and (2) areas where grass and rocky shores were not contiguous, rather the grass lower limits were several hundreds of meters back from the shore. In both grass and no grass areas, in the intertidal zone, all benthic organisms and all potential OM sources…

Diel feeding habits of juveniles of Mullus surmuletus (Linneo, 1758) in the lagoon of the Stagnone di Marsala (Western Sicily, Italy)

Diet composition, feeding rhythm, gastric evacuation rate and daily ration were investigated in juvenile Mulhis surmuletus (Linneo, 1758). Fish were collected in the lagoon of the Stagnone di Marsala in western Sicily, in July 1995, during a 24 h sampling period. Copepoda, Polychaeta, Amphipoda and Tanaidacea were shown to be the most frequent prey items. The feeding index values showed two different daily feeding times. A unimodal trend in the daily rhythm of food consumption was derived, with a peak in feeding between 1200 and 2000 h. Gastric evacuation in juvenile M. surmuletus is best described by an exponential model, with a gastric evacuation rate R = 0.66 g h-1 (r = 0.88) (T = 24.45 …

Predicting the effectiveness of oil recovery strategies in the marine polluted environment

Abstract Many recent studies have focused their attention on the physiological stress experienced by marine organisms in measuring ecotoxicological responses. Here we suggest a new approach for investigating the effects of an anthropogenic pollutant on Life-History (LH) traits of marine organisms, to provide stakeholders and policy makers an effective tool to evaluate the best environmental recovery strategies and plans. A Dynamic Energy Budget (DEB), coupled with a biophysical model was used to predict the effects of a six-month oil spill on Mytilus galloprovincialis' LH traits and to test two potential recovery strategies in the central Mediterranean Sea. Oxygen consumption rates were use…

Effects of Nautical Traffic and Noise on Foraging Patterns of Mediterranean Damselfish (Chromis chromes)

Chromis chromis is a key species in the Mediterranean marine coastal ecosystems where, in summer, recreational boating and its associated noise overlap. Anthropogenic noise could induce behavioural modifications in marine organisms, thereby affecting population dynamics. In the case of an important species for the ecosystem like C. chromis, this could rebound on the community structure. Here, we measured nautical traffic during the summer of 2007 in a Southern Mediterranean Marine Protected Area (MPA) and simultaneously the feeding behaviour of C. chromis was video-recorded, within both the no-take A-zone and the B-zone where recreational use is allowed. Feeding frequencies, escape reaction…

Thermal adaptation and physiological responses to environmental stress in tunicates

Understanding the multifaceted nature of environmental fluctuations is crucial to predicting the physiological adjustments utilised by organisms in resisting or adapting to changes over time. Here we investigate the effects of 2 environmental stressors on tunicates, whose fitness can have important repercussions on the quality of habitat. Specifically, we report respiration rate (RR), clearance rate (CR), and assimilation efficiency (AE) of the ascidian Styela plicata in response to a range of temperatures and varying food availability. Temperature-dependent RR was observed only within a portion of the thermal window of the species. Significant differences in clearance rates were detected a…

Impacts of marine aquaculture at large spatial scales: evidences from n and p catchment loading and phytoplankton biomass

International audience; While several studies point at off-shore aquaculture as a possible source of impacts on the local marine environment, very few have analysed its effects at large scales such as at the bay, gulf or basin levels. Similar analyses are hampered by the multiple sources of disturbance that may concomitantly affect a given area. The present paper addresses these issues taking the Gulf of Castellammare (Southern Tyrrhenian Sea) as an example. Nitrogen (N) and phosphorous (P) loads were calculated for the period 1970-2007, and compared to chlorophyll-a concentration as measured inside and outside the Gulf over the same period. Results indicate that N and P catchment loading h…

Evenness, biodiversity, and ecosystem function of intertidal communities along the Italian coasts: Experimental short-term response to ambient and extreme air temperatures

Biodiversity can promote ecosystem functioning in both terrestrial and marine environments, emphasizing the neces- sity ofbiodiversity conservation in order to preserve critical ecosystem functions and associated services. However, the role of biodiversity in buffering ecosystem functioning under extreme events caused by climate change remains a major scientific issue, especially for intertidal systems experiencing stressors from both terrestrial and marine drivers. We performed a regional-scale field experiment along the Italian coast to investigate the response of unmanipulated intertidal communities (by using a natural biodiversity gradient) to low tide aerial exposure to both ambient an…

Use of stable isotopes to investigate dispersal of waste from fish farm as a function of hydrodynamics.

Stable isotopes were used to examine differential effects of fish farm waste on the water column and sediments. To achieve this objective, we chose 3 marine fish farms located along the coast of Sicily (Mediterranean Sea) as point-source disturbances, and a control area. The hypothesis that carbon and nitrogen isotope composition of particulate (POM) and sedimentary (SOM) organic matter varied with increasing distance (from cages to 1000 m) was tested at 3 levels of hydrodynam- ics: low (mean velocity of current (MVC) ~12 cm s -1 ), intermediate (MVC ~22 cm s -1 ), and high (MVC ~40 cm s -1 ). Different isotopic signals from allochthonous (fish waste) over natural (phytoplankton, terrigenou…

Influence of hydrodynamic conditions on the production and fate ofPosidonia oceanicain a semi-enclosed shallow basin (Stagnone di Marsala, Western Sicily)

An integrated approach using hydrodynamic and transport numerical models, lepidochronology and stable isotope analysis was used to investigate how local hydrodynamic conditions influence the primary production and fate of the seagrass Posidonia oceanica in a Mediterranean semi-enclosed marine system (Stagnone di Marsala). The water mass exchange aptitude of different sectors of the basin was analysed, and data collected were used to select two sectors (colonized by Posidonia oceanica showing the lowest and highest water exchange values) for biological analyses. According to the mean dispersal coefficient differences simulated by the hydrodynamic model, growth rate and primary production of …

Biological traits, geographic distributions, and species conservation in aquatic ecosystems

Aquatic ecosystems (both marine and freshwater) have long served as model systems for exploring the role of environmental stressors on organismal performance and survival, the biogeographic distributions of populations and species, and ultimately the diversity, functioning, and stability of ecosystems (Adams, 2002; Forbes, 1887; MacArthur & Wilson, 2001; Paine, 1969; Somero et al., 2017 ). Climate change, alien species invasions, land use change, urbaniza-tion, and other anthropogenic impacts have all been demonstrated to impair aquatic ecosystems at multiple levels of biological orga-nization within aquatic ecosystems (Karr, 1991; Doney et al., 2012; Harley et al., 2006; Poloczanska et…

Microplastics and the functional traits of fishes: A global meta‐analysis

Over the years, concern about the effects of microplastics has grown. Here, we answered the main question “What are the impacts of microplastics on the functional traits of fish species?” through a meta-analysis. The general impact of microplastic exposure on the functional traits of fishes and specifically on eight variables, namely, behaviour, development, fecundity, feeding, growth, health, hatching and survival was explored. Subgroup analyses were performed to detect correlations between the impact of microplastics and the following factors: species, life stage, habitat, water column habitat, day of exposure to microplastics and microplastic size, type and shape. A meta-regression analy…

Seascape connectivity of European anchovy in the Central Mediterranean Sea revealed by weighted Lagrangian backtracking and bio-energetic modelling

AbstractEcological connectivity is one of the most important processes that shape marine populations and ecosystems, determining their distribution, persistence, and productivity. Here we use the synergy of Lagrangian back-trajectories, otolith-derived ages of larvae, and satellite-based chlorophyll-a to identify spawning areas of European anchovy from ichthyoplanktonic data, collected in the Strait of Sicily (Central Mediterranean Sea), i.e., the crucial channel in between the European and African continents. We obtain new evidence of ecosystem connectivity between North Africa and recruitment regions off the southern European coasts. We assess this result by using bio-energetic modeling, …

Bacterial communities in sediment of a Mediterranean marine protected area

Biodiversity is crucial in preservation of ecosystems, and bacterial communities play an indispensable role for the functioning of marine ecosystems. The Mediterranean marine protected area (MPA) “Capo Gallo–Isola delle Femmine” was instituted to preserve marine biodiversity. The bacterial diversity associated with MPA sediment was compared with that from sediment of an adjacent harbour exposed to intense nautical traffic. The MPA sediment showed higher diversity with respect to the impacted site. A 16S rDNA clone library of the MPA sediment allowed the identification of 7 phyla: Proteobacteria (78%), Firmicutes (11%), Acidobacteria (3%), Actinobacteria (3%), Bacteroidetes (2%), Planctomyc…

Functional responses of intertidal bivalves to repeated sub-lethal, physical disturbances

In coastal habitats, physical disturbances of benthic organisms can be caused by natural events like wave-born objects and human activity like trampling, and these disturbances can be sub-lethal (e.g., resulting in the organism's displacement). We know little of how sessile organisms respond to physical disturbances such as displacements. Using Mytilaster minimus, a mussel that is native to the Mediterranean Sea, we tested how byssus production and oxygen uptake rates changed in response to different frequencies of disturbance events (10-60 events h-1). Mussels increased oxygen uptake rates but not byssus production with increasing disturbance frequencies (50-60 events h-1). Our results sho…

Cumulative climatic stressors strangles marine aquaculture: Ancillary effects of COVID 19 on Spanish mariculture

9 pages, 8 figures

Seagrasses along the Sicilian coasts

All seagrass species known from the Mediterranean basin have been recorded along the Sicilian coast, where studies have been carried out at a very local scale and information is fragmented or confined to the grey literature. The objective of this article is to summarise and evaluate current knowledge on seagrass species on the Sicilian coasts, providing an overview of species distribution, genetic diversity, biology and ecology, based on the literature and unpublished data. Most literature studies have been carried out on Posidonia oceanica meadows because of their wide distribution, complexity and ecological importance. In this study, the analyses carried out on P. oceanica structural and …

Sources of organic matter for inter-tidal consumers in Ascophyllum-shores (Sw Iceland): a multi stable isotope approach.

Stable isotopes were used to examine the origin of organic matter in Icelandic Ascophyllum-based habitats, the role of different organic matters in filling intertidal food webs and the food preferences of the most abundant suspension feeders, grazers and predators. We selected three intertidal sites on the SW coast of Iceland where we sampled in early September 2004, organic matter sources (POM, SOM and most abundant primary producers, A. nodosum and F. vesciculosus) and the most abundant macrofauna species (barnacles, mussels, gastropods, sponge and crabs). Even though the primary production (Ascophyllum-based) was the same at the three study sites, the isotopic composition of common-among…

A False Sense of Protection: Recreational Uses and Illegal Behavior in a Mediterranean Marine Protected Area and Implications for Management.

Over the last 35 years, at both the European and the Italian level, great efforts have been made to increase the number of Marine Protected Areas (MPAs): they are considered an effective tool for protecting oceans and biodiversity. In recent years, MPAs have become more than simply tools to improve marine conservation. In fact, their management agencies are actively involved in the sustainable development of nearby communities through the promotion of recreational activities (boating, snorkeling, diving). Even if the recreational uses of the marine environment are generally considered benign, they can potentially be highly detrimental for species and their habitats. As a result, these activ…

Factors affecting fish assemblages associated with gas platforms in the Mediterranean Sea

Abstract Understanding the role played by offshore platforms in marine ecosystems is acquiring increasing importance worldwide. In this work, underwater visual census techniques were applied to describe spatial and temporal patterns of fish assemblages associated with extractive platforms. Data were collected during three seasons according to the following spatial factors: Location (Adriatic and Ionian Seas), Depth (0–6 m and 12–18 m) and Distance from the platform (external and internal). Both univariate and multivariate analyses showed highly significant differences for each factor assessed in this study, as well as for the interaction among said factors. Results indicated that artificial…

Fish functional traits are affected by hydrodynamics at small spatial scale

The Mediterranean damselfish Chromis chromis is a species with a broad distribution found both in the Mediterranean Sea and Eastern Atlantic as far south as the coast of Angola. We hypothesized that the species may have significant functional morphological plasticity to adapt along a gradient of environmental conditions. It is a non-migratory zooplanktivorous species and spends the daytime searching for food in the middle of the water column. Therefore, local hydrodynamics could be one of the environmental factors affecting traits of C. chromis with repercussions at the population level. We compared the body condition, individual growth and body shapes of damselfish collected under two diff…

Origin and distribution of suspended organic matter as inferred from carbon isotope composition in a Mediterranean semi-enclosed marine system

The origin and distribution of suspended organic matter, the trophic features and the stable carbon isotopic composition of particulate organic carbon (POC) were studied monthly in a Western Mediterranean semi-enclosed basin. Sampling stations were selected as a function of wind-exposure and the degree of vegetation cover and then compared with an adjacent unvegetated site. The predominant vegetation was seagrass (Posidonia oceanica and Cymodocea nodosa) and Caulerpa prolifera. Water samples were analyzed for total suspended matter (inorganic and organic fractions), photosynthetic pigments (chlorophyll-a and phaeopigments), dissolved organic carbon, particulate organic carbon and their isot…

Cultivation of the Mediterranean amberjack, Seriola dumerili (Risso, 1810), in submerged cages in the Western Mediterranean Sea

The growth rate, survival and food conversion ratio (FCR) of the Mediterranean amberjack (Seriola dumerili, Risso, 1810) was ascertained in cultivation using submerged net cages in the Gulf of Castellammare (NW Sicily), from September to December 1994. Two net cages (volume = 75 m3) were placed at a depth of 10 m in a sheltered area 1000 m off the coast. Juveniles (mean total length = 141.4 ± 34 2 mm; mean total wet weight = 48 ± 28.1 g) were caught in the gulf under floating wreckage with a purse seine and transplanted to the cages (n = 800 per cage) in August. Fish in one cage, group A, were fed with fish scraps whilst fish in group B were fed with pellets The total length and body wet we…

Amount, composition, and spatial distribution of floating macro litter along fixed trans-border transects in the Mediterranean basin

Marine litter is a major source of pollution in the Mediterranean basin, but despite legislative requirements, scant information is available for the ongoing assessment of this threat. Using higher size classes as proxy for litter distribution, this study gave a synoptic estimation of the amount, composition, and distribution of floating macro-litter in the Mediterranean. The average amount of macro-litter was in a range of 2-5 items/km2, with the highest in the Adriatic basin. Seasonal patterns were present in almost all study areas and were significant in the Ligurian Sea, Sardinian-Balearic basin, and Central Tyrrhenian Sea. Plastic accounted for > 80% of litter in all areas and seaso…

Biostimulation of in situ microbial degradation processes in organically-enriched sediments mitigates the impact of aquaculture

Fish farm deposition, resulting in organic matter accumulation on bottom sediments, has been identified as among the main phenomena causing negative environmental impacts in aquaculture. An in situ bioremediation treatment was carried out in order to reduce the organic matter accumulation in the fish farm sediments by promoting the natural microbial biodegradation processes. To assess the effect of the treatment, the concentration of organic matter in the sediment and its microbial degradation, as well as the response of the benthic prokaryotic community, were investigated. The results showed a significant effect of the treatment in stimulating microbial degradation rates, and the consequen…

The carrying capacity for Mediterranean bivalve suspension feeders: evidence from analysis of food availability and hydrodynamics and their integration into a local model

Abstract In order to assess the carrying capacity of two Mediterranean areas, the Incze et al. model and its modification were applied. Our measures were carried out in the Gulf of Gaeta (Central MED), where mussels ( Mytilus galloprovincialis ) are intensively cultivated (production of approximately 200 t per year) and the Gulf of Castellammare (Southern MED), where bivalve culture is not widely practised. Velocities of water current and in field filtration rates were measured in each area. Total suspended matter (TSM), suspended chlorophyll-a (CHLa), lipid, protein and carbohydrate concentrations in the particulate were measured seasonally and used as tools to evaluate the trophic status …

Meiofauna and benthic microbial biomass in a semi-enclosed mediterranean marine system (Stagnone di Marsala, Italy)

Microbial and meiofaunal dynamics and their relationships with the biochemical composition of the sedimentary organic matter were investigated in a semi-enclosed marine system (Marsala lagoon, Western Sicily, Mediterranean Sea). Sediment samples were collected on a monthly basis from March 1996 to February 1997 in four stations located along a N–S transect characterized by different hydrodynamic regimes. Total sedimentary organic matter concentration ranged from 5.681.11 to 156.2812.63 mg g1, while the biopolymeric fraction of organic carbon (BPC, measured as sum of the lipids, carbohydrates and proteins) accounted for only a small fraction (24%) of total organic matter. Total meiofaunal de…

Unveiling the Relationship Between Sea Surface Hydrographic Patterns and Tuna Larval Distribution in the Central Mediterranean Sea

Thunnus thynnus (Atlantic bluefin tuna, ABT) and other tuna species reproduce in the Mediterranean Sea during the summer period. Despite the Central Mediterranean Sea, the Strait of Sicily in particular, being a key spawning site for many tuna species, little is known on the effects of oceanographic variability on their larval distribution in this area. The abundance and presence-absence of larval specimens for three tuna species (ABT, bullet tuna and albacore) were modeled in order to examine their relationships with environmental factors, by analysing historical in situ information collected during seven annual surveys (2010–2016). The results revealed that most tuna larvae for the three …

The relationship between food availability and growth in Mytilus galloprovincialis in the open sea (southern Mediterranean)

Abstract With the aim of gathering information about the possibility of culturing mussels ( Mytilus galloprovincialis ) in a south Mediterranean oligotrophic area, different lots of mussels were placed in culture at depths of −5 m and −15 m and their growth monitored on a monthly basis. Temperature and salinity were measured in situ and water samples were collected at different depths each month. Total suspended matter (TSM) and its inorganic (ISM) and organic (OSM) fractions were analysed by gravimetry and loss on ignition. Photosynthetic pigments (chlorophyll- a and phaeopigments), particulate organic carbon (POC) and nitrogen (PON), particulate carbohydrate (CHO), protein (PRT) and lipid…

The effect of mariculture facilities on biochemical features of suspended organic matter (southern Tyrrhenian, Mediterranean)

Abstract A comparison of a Mediterranean aquaculture impacted area and control areas was made to assess the effect of fish farm waste discharge on the biochemical features of the water column. Trophic variables commonly used in marine ecology such as total suspended matter, suspended chlorophyll- a , biochemical features of particulate organic matter (proteins, lipids and carbohydrates) and biopolymeric carbon were chosen as the best descriptors of trophic conditions. An initial analysis of data from the impact area was carried out in order to test the effect of farm waste using a gradient of distances downstream from the fish farm cages (50 m, 300 m, 1000 m). The results were then compared…

Dynamic Energy Budget model parameter estimation for the bivalve Mytilus californianus: Application of the covariation method

Dynamic Energy Budget (DEB) models serve as a powerful tool for describing the flow of energy through organisms from assimilation of food to utilization for maintenance, growth and reproduction. The DEB theory has been successfully applied to several bivalve species to compare bioenergetic and physiological strategies for the utilization of energy. In particular, mussels within the Mytilus edulis complex (M. edulis, M. galloprovincialis, and M. trossulus) have been the focus of many studies due to their economic and ecological importance, and their worldwide distribution. However, DEB parameter values have never been estimated for Mytilus californianus, a species that is an ecological domin…

Temperature increases, hypoxia, and changes in food availability affect immunological biomarkers in the marine mussel Mytilus galloprovincialis

Temperature increases, hypoxia, and changes in food availability are predicted to occur in the future. There is growing concern for the health status of wild and farmed organisms, since environmental stressors alter organism functions, and elicit coordinated physiological responses for homeostasis. Mussels are good bioindicators of environmental conditions. Their ability to maintain unaltered immunosurveillance under adverse environmental conditions may enhance their survival capability. Few studies are currently concerned with the relationships and feedback among multiple stressors. Here, food concentration, temperature, and oxygenation treatments were evaluated for their effects on immune…

Dynamic Energy Budget parameters of Brachidontes pharaonis, a lessepsian bivalve in the Mediterranean Sea.

Dynamic Energy Budget (DEB) models are used for describing the flow of energy through organisms. The most important and powerful aspect of DEB theory is that inter-specific differences can be captured in the same model by comparison of parameter values. However, estimation of these parameters is complicated and can often not be done with direct empirical measurements alone. Here, we present DEB parameter estimates obtained by combining both experimental and literature data for the filter feeder Brachidontes pharaonis, which is considered one among the 100 worst invasive marine species in the Mediterranean Basin. We utilize a statistical procedure based on the covariation method to obtain pa…

Animal fouling as an indicator of water quality in Mediterranean fish farmed areas

In the Mediterranean, most of the fish farm plants are located in very deep water columns (>20-30 m) and are characterised by energetic hydrodynamic regime levels. Thus, the effects of farming load...

Thinking beyond organism energy use: a trait-based bioenergetic mechanistic approach for predictions of life history traits in marine organisms

The functional trait-based bioenergetic approach is emergent in many ecological spectra, from the conservation of natural resources to mitigation and adaptation strategies in a global climate change context. Such an approach relies on being able to exploit mechanistic rules to connect environmental human-induced variability to functional traits (i.e. all those specific traits defining species in terms of their ecological roles) and use these to provide estimates of species life history traits (LH; e.g. body size, fecundity per life span, number of reproductive events). LHs are species-specific and proximate determinants of population characteristics in a certain habitat. They represent the …

Boat traffic in Lampedusa waters (Strait of Sicily, Mediterranean Sea) and its relation to the coastal distribution of common bottlenose dolphin (Tursiops truncatus).

The volume of boat traffic and its potential connection to the coastal distribution of the common bottlenose dolphin (Tursiops truncatus) was evaluated off Lampedusa Island (Strait of Sicily). From July to September 2006 daily surveys were carried out at eight sites along the coast, three times a day, to assess the number, type, and size of boats moving, fishing, or stationed in Lampedusa waters. The study area was divided into four geographic areas: northwest, northeast, southwest, and southeast. Data were analyzed to determine the difference in the number of boats among the areas, sampling months, and times of day. The presence of dolphins was monitored by standardized land-based observat…

Spatial and temporal changes of suspended matter in relation to wind and vegetation cover in a mediterranean shallow coastal environment

Seasonal and spatial changes in seston, (POC), particulate organic carbon, (PON) particulate organic nitrogen and chlorophyll-a concentrations were studied on a monthly basis in a Mediterranean shallow coastal area (Stagnone di Marsala, Western Sicily) in order to gather information on factors controlling particulate organic matter distribution and composition. Seston concentration and composition were connected to the main physicochemical and biological driving factors, such as temperature, salinity, dissolved oxygen, wind-speed and biomass of submerged vegetation. The Stagnone di Marsala is characterized by high temperatures with strong seasonality (range: 11-28°C), while values ranged fr…

How ocean acidification can benefit calcifiers.

Reduction in seawater pH due to rising levels of anthropogenic carbon dioxide (CO2) in the world's oceans is a major force set to shape the future of marine ecosystems and the ecological services they provide [1,2]. In particular, ocean acidification is predicted to have a detrimental effect on the physiology of calcifying organisms [3]. Yet, the indirect effects of ocean acidification on calcifying organisms, which may counter or exacerbate direct effects, is uncertain. Using volcanic CO2 vents, we tested the indirect effects of ocean acidification on a calcifying herbivore (gastropod) within the natural complexity of an ecological system. Contrary to predictions, the abundance of this cal…

The importance of thermal history: costs and benefits of heat exposure in a tropical, rocky shore oyster.

Although thermal performance is widely recognized to be pivotal in determining species' distributions, assessment of this performance is often based on laboratory acclimated individuals, neglecting their proximate thermal history. The thermal history of a species sums the evolutionary history and, importantly, the thermal events recently experienced by individuals, including short-term acclimation to environmental variations. Thermal history is perhaps of greatest importance for species inhabiting thermally challenging environments and therefore assumed to be living close to their thermal limits, such as in the tropics. To test the importance of thermal history the responses of the tropical…

Mussels as a model system for integrative ecomechanics.

Copyright © 2015 by Annual Reviews. All rights reserved. Mussels form dense aggregations that dominate temperate rocky shores, and they are key aquaculture species worldwide. Coastal environments are dynamic across a broad range of spatial and temporal scales, and their changing abiotic conditions affect mussel populations in a variety of ways, including altering their investments in structures, physiological processes, growth, and reproduction. Here, we describe four categories of ecomechanical models (biochemical, mechanical, energetic, and population) that we have developed to describe specific aspects of mussel biology, ranging from byssal attachment to energetics, population growth, an…

Collating science-based evidence to inform public opinion on the environmental effects of marine drilling platforms in the Mediterranean Sea.

Abstract The use of rigorous methodologies to assess environmental, social and health impacts of specific interventions is crucial to disentangle the various components of environmental questions and to inform public opinion. The power of systematic maps relies on the capacity to summarise and organise the areas or relationships most studied, and to highlight key gaps in the evidence base. The recent Italian technical referendum (2016) – a public consultation inviting people to express their opinion by voting to change the rules on the length of licence duration and the decommissioning of offshore oil and gas platform drilling licences – inspired the creation of a systematic map of evidence…

The effect of Ruppia cirrhosa features on macroalgae and suspended matter in a Mediterranean shallow system

Relationships among chemical–physical features, total gross suspended organic matter, coverage of the seagrass Ruppia cirrhosa and its associated algal community in eight ponds of a saltworks system of western Sicily (Mediterranean Sea) were investigated in spring and summer 2004. All biological features varied both at different levels of seagrass coverage and between seasons. A low algal diversity (46 taxa, 14.75 ± 1.41 on average) was highlighted; algal coverage and species richness showed to be negatively correlated. Ruppia cirrhosa coverage was negatively correlated with algal coverage, but positively correlated with species richness. Moreover, a significant correlation among R. cirrhos…

Concurrent environmental stressors and jellyfish stings impair caged European sea bass (Dicentrarchus labrax) physiological performances

9 pages, 5 figures, 4 tables

The sanitation service of seagrasses – Dependencies and implications for the estimation of avoided costs

Seagrasses are capable of sanitizing coastal seawaters polluted by fecal bacteria. In this work, the reduction of Enterococci concentration in the presence of a seagrasses’ assemblage (Pacific Ocean) was related to the decrease in the probability of gastroenteritis. A linear model fitted to data extracted from the literature showed a 20% reduction of this probability in the presence of these plants. Seagrass sanitation effect was estimated to allow avoiding ca. 24 million gastroenteritis cases/year, globally. Considering a global cost of gastroenteritis of ca. US$ 372 million/year, the global avoided cost, assuming that the sanitation service was always effective, was estimated to be ca. US…

Trophic habits of Muscardinus avellanarius (Mammalia Gliridae) as revealed by multiple stable isotope analysis

Multiple stable isotope analysis was used to investigate the diet of the common dormouse Muscardinus avellanarius in a mixed Mediterranean forest. δ13C and δ15N values of dormouse tissues compared to those of the vegetal and animal sources available in the area showed that the isotopic composition of both adult and juvenile M. avellanarius could be explained by a mixture of vegetal and animal organic matter. The isotopic composition of the assimilated nitrogen was mostly explained by the organic matter from flora. Among the vegetal components, flowers of Fagus silvatica, Quercus robur, and the rarer Crataegus oxyacantha, berries of Ilex aquifolium and acorns of Q. robur were found in the ad…

Integrating mechanistic models and climate change projections to predict invasion of the mussel, Mytilopsis sallei, along the southern China coast

Species invasion is an important cause of global biodiversity decline and is often mediated by shifts in environmental conditions such as climate change. To investigate this relationship, a mechanistic Dynamic Energy Budget model (DEB) approach was used to predict how climate change may affect spread of the invasive mussel Mytilopsis sallei, by predicting variation in the total reproductive output of the mussel under different scenarios. To achieve this, the DEB model was forced with present-day satellite data of sea surface temperature (SST) and chlorophyll-a concentration (Chl-a), and SST under two warming RCP scenarios and decreasing current Chl-a levels, to predict future responses. Und…

Estimation of dynamic energy budget parameters for the Mediterranean toothcarp (Aphanius fasciatus)

Organisms adopt different sets of physiological, behavioural and morphological trade-offs in order to cope with natural environmental fluctuations. This has consequential rebounds on ecological processes and population dynamics. Such aspects become crucial for sex-dimorphic species, where sex-specific growth variation could mirror different tactics both in energy acquisition and investment between maximum female and male body size with cascading effects on population demography. To date, different approaches have been used in order to understand the causes of individual growth rate changes in ectotherm indeterminate growers, most of which failed. Here, we propose the use of a mechanistic mo…

The Synergistic Impacts of Anthropogenic Stressors and COVID-19 on Aquaculture: A Current Global Perspective

13 pages, 6 figures, 2 tables.-- This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License

Climate change, marine policy and the valuation of Mediterranean intertidal ecosystems

This commentary describes a gap in the qualitative and quantitative knowledge of the provision of benefits to humans from the intertidal ecosystems of the Mediterranean and offers a framework for quantification of the benefits provided by these systems. The identification of such benefits, understanding their spatial distribution and their subsequent quantification will be critical to the design of policy responses to future climate change, sea level rise and seawater acidification. A baseline understanding of the current state of ecosystem functions, as well as of the provision of related ecosystem services, is therefore needed. Further, researchers must strive to generate forecasts of the…

Local consumers are the first line to control biological invasions: a case of study with the whelk Stramonita haemastoma (Gastropoda: Muricidae)

The increasing spread of invasive species in the Mediterranean Sea determines several alterations in local food webs, changing the feeding habits of native organisms. The whelk Stramonita haemastoma is a widespread Mediterranean gastropod that consumes bivalves, barnacles and limpets. Previous studies showed a shift in its diet from the bivalve Mytilaster minimus to the invasive mussel Brachidontes pharaonis, presumably due to a higher energy gain. Here we tested whelks’ preference among natives and a novel prey, calculating the profitability ratio, and integrating those results with biochemical analysis on prey tissues and the routine metabolism of the whelks. Further, we used the scaled f…

Effect of the presence of the shore crab, Carcinus maenas, on the burrowing behaviour and clearance rate of the common cockle, Cerastoderma edule

Bivalves demonstrate various morphological and behavioural adaptations to reduce the risk of being attacked by predators. This paper examines how the presence of the crab Carcinus maenas (L.), a natural predator of the cockle Cerastoderma edule (L.), affects its burrowing depth and clearance or feeding rate. Cockles were placed in experimental tanks and treated with three levels of predatory disturbance: (1) unfed crab loose inside the tank, (2) unfed crab inside a cage suspended in the water column and (3) no crab present. Cockles’ burrowing depth was measured in two sediment types: mud and sand. Cockles burrowed more deeply in treatments with no crabs. Burrowing depth in sand was signific…

Influence of environmental factors and biogenic habitats on intertidal meiofauna

This study investigated the influence of physical and chemical conditions and biotic factors on the distribution and diversity of meiofauna in intertidal zone along a geographical gradient. At 11 sites along the Italian coast, we studied the concurring role of environmental variables, trophic resources and the presence of habitat-forming species (macroalgae vs. mussels) in controlling the meiofaunal communities. The increase of water temperature combined with local thermal conditions was associated with a decrease in nematodes and copepods, with a consequent decrease in meiofaunal abundance towards the south. However, the increase in salinity, as geographical gradient decreases, and local t…

Dynamic Energy Budget provides mechanistic derived quantities to implement the ecosystem based management approach

Abstract The on-going climate change threats are rapidly growing at both global and local scales, affecting ecosystems, societies and economies by altering natural distribution and productivity of key commercial species. Although the ecosystem based management (EBM) focuses on ecosystem equilibria, to provide realistic management measures for important activities at sea such as fisheries and aquaculture, there is a need of quantities; mechanistic approaches are suggested as reliable solutions. Here, a Dynamic Energetic Budget (DEB) application studies the link between environmental change (temperature forecasted increasing scenario in a context of COP 21 [Paris climate conference Agreement]…

The fouling community as an indicator of fish farming impact in Mediterranean

Fouling species richness, abundance and composition and biomass were chosen as the descriptors of effect of fish farm organic enrichment. The study was carried out in September 2004 in the Gulf of Castellammare (South Tyrrhenian, Mediterranean). The fouling species were sampled from plastic buoys spaced throughout the study area both up- [UP] and down-stream [DOWN]. The results showed that fouling community responded to the chronic input of allochthonous organic matter experiencing local changes more or less significantly with regard to abundance, species composition and general community diversity. Upper fouling would work as a first filter naturally opposed by environment resistance assim…

The detrimental consequences for seagrass of ineffective marine park management related to boat anchoring.

Posidonia oceanica (L.) Delile meadows are recognized as priority habitat for conservation by the EU Habitats Directive. The La Maddalena Archipelago National Park (Mediterranean Sea) P. oceanica meadow. , the dominant coastal habitat of the area, is mostly threatened by boat anchoring. 12. years after the establishment of mooring fields and anchoring restrictions, a study was conducted to measure their effectiveness on the conservation of seagrass and the mitigation of anchoring damage. We found that: (i) the condition of P. oceanica was disturbed, both in the mooring fields and in control locations; (ii) mooring fields and anchoring restrictions did not show to be an efficient system for …

Carbon and nitrogen stable isotopic inventory of the most abundant demersal fish captured by benthic gears in southwestern Iceland (North Atlantic)

International audience; Stable isotopes (δC and δN) were used to examine the origin of organic matter for the most representative demersal species of the SW Icelandic fishery, accounting for over 70% of landings of those species in the North Atlantic. Samples were collected during a 2-week period in early September 2004 from landings and directly during fishing cruises. Stable isotopes showed that particulate organic matter and sedimentary organic matter were at the base of the food web and appeared to fill two different compartments: the pelagic and the benthic. The pelagic realm was composed of only capelin and sandeel; krill and redfish occupied an intermediate position between pelagic a…

Functional consequences of prey acclimation to ocean acidification for the prey and its predator

Ocean acidification is the suite of chemical changes to the carbonate system of seawater as a consequence of anthropogenic carbon dioxide (CO2) emissions. Despite a growing body of evidences demonstrating the negative effects of ocean acidification on marine species, the consequences at the ecosystem level are still unclear. One factor limiting our ability to upscale from species to ecosystem is the poor mechanistic understanding of the functional consequences of the observed effects on organisms. This is particularly true in the context of species interactions. The aim of this work was to investigate the functional consequence of the exposure of a prey (the mussel Brachidontes pharaonis) t…

Caulerpa racemosa var. cylindracea as a potential source of organic matter for benthic consumers: evidences from a stable isotope analysis.

Caulerpa racemosa var. cylindracea has been invading various types of substrates in wide areas throughout the Mediterranean Sea. However, the effects of the distribution of this alga on zoobenthos are scanty. The aim of this study was to evaluate the effects of C. racemosa invasion on the feeding habits of some zoobenthic taxa inhabiting the upper infralittoral rocky shores. This was done by identifying the isotopic N and C ratios of several potential food sources and testing differences in isotope composition among the taxa collected from areas invaded and not-invaded areas by C. racemosa. Results suggest that C. racemosa detritus was a significant food source for the polychaete Syllis pro…

Pinger affects fish catch efficiency and damage to bottom gill nets related to bottlenose dolphins

There is some evidence that the presence of Tursiops truncatus in fishing areas represents a real economic threat to fishermen due the dolphin feeding on the entangled fish, damaging the nets and reducing the fish catch. We have carried out experiments to assess the efficiency of a pinger in decreasing the interaction between the dolphins and fishing nets, in a fishing area off the coast of southern Italy, where Tursiops truncatus is frequently observed to interact with bottom gill nets. Two identical monofilament bottom gill nets (900 m long), one equipped with pingers and the other without, were used to measure the effect of these pingers on the abundance of the catch and net damage. For …

Marine Animal Forests

Fish diversity associated with gas platforms: Evaluation of two underwater visual census techniques

Se estudiaron las comunidades ícticas asociadas con plataformas extractivas mediante dos técnicas de censos visuales submarinos: transectos y conteos de puntos móviles. Los datos se tomaron a cuatro profundidades en tres diferentes plataformas situadas en el Mar Jónico (Crotone, Italia). Se registraron un total de 25 especies de peces pertenecientes a 12 familias. Se observaron diferencias significativas entre ambos métodos de muestreo y las profundidades en cuanto a la estructura de la comunidad íctica, la riqueza de especies y la abundancia de peces. El promedio de peces registrado con el método de transectos siempre fue mayor que el registrado mediante puntos móviles, mientras que lo con…

Effect of boat noise on the behaviour of bluefin tuna Thunnus thynnus in the Mediterranean Sea

The effect of boat noise on the behaviour of bluefin tuna Thunnus thynnus was investi- gated in the Egadi Islands, Sicily, during spring 2005 using a fixed tuna trap set near shipping routes. Tuna behaviour was observed when exposed to both natural ambient sound and sound generated by hydrofoil passenger ferries, small boats and large car ferries. Acoustical and behavioural analyses were conducted with and without extraneous sound to define a list of behavioural categories. Each vessel produced different engine sounds with regard to their composition and bandwidth, and all were distinctly different from ambient sound levels. In the absence of boat noise, tuna assumed a con- centrated coordi…

The duality of ocean acidification as a resource and a stressor

Ecologically dominant species often define ecosystem states, but as human disturbances intensify, their subordinate counterparts increasingly displace them. We consider the duality of disturbance by examining how environmental drivers can simultaneously act as a stressor to dominant species and as a resource to subordinates. Using a model ecosystem, we demonstrate that CO2-driven interactions between species can account for such reversals in dominance; i.e., the displacement of dominants (kelp forests) by subordinates (turf algae). We established that CO2 enrichment had a direct positive effect on productivity of turfs, but a negligible effect on kelp. CO2 enrichment further suppressed the …

Aquaculture effects on some physical and chemical properties of the water column: A meta-analysis

More than 30 peer-reviewed articles (1980–2005) were analysed using meta-analytical reviewing techniques, and about 340 study cases were used to test whether aquaculture facilities had any effects on physical and chemical variables. The analysis tested differences between experimental conditions vs. chosen-by-author controls. Across all study cases, cultivated organisms (fish, shrimps and bivalves) did not have any clear effects on the water temperature and salinity. Dissolved oxygen also was found to be unaffected by aquaculture practices. On the other hand, crowding led to significant pH variations, which was more accentuated in shrimp (d+ = 0.66;P 0.05).Water transparency and turbidity w…

Monitoring of persistent organic pollutants in the polar regions: knowledge gaps & gluts through evidence mapping

Persistent organic pollutants (POPs) are widespread compounds that accumulating in polar regions canalise through trophic webs. Although several dozens of studies have been carried out in the last decades, the information is generally scattered across a large number of literature sources. This does not allow an efficient synthesis and constraints our understanding on how address future monitoring plans and environmental conservation strategies on the Polar Regions with respect to POPs. Thus, here, we present the outcome of a systematic map (SM) to scope, screen and chart evidences from literature dealing with POPs in Polar regions. The SMs strive to produce rigorous guidelines and have rece…

The buffer effect of canopy-forming algae on vermetid reefs' functioning: A multiple stressor case study.

Abstract Biodiversity plays a key role for our planet by buffering ongoing and future changes in environmental conditions. We tested if canopy-forming algae enhancing biodiversity (CEB) in a Mediterranean intertidal reef ecological community could alleviate the effect of stressors (heat waves and pollution from sewage) on community metabolic rates (as expressed by oxygen consumption) used as a proxy of community functioning. CEB exerted a buffering effect related to the properties of stressor: physical-pulsing (heat wave) and chronic-trophic (sewage). After a simulated heat wave, CEB was effective in buffering the impacts of detrimental temperatures on the functioning of the community. In r…

Changes in behavioural response of Mediterranean Seabass (Dicenthratus labrax L.) under different feeding distributions

Captive-induced behavioural deviations may involve many aspects of fish behaviour such as swimming activity and enhancement of individual aggressiveness. We studied seabass (Dicentrarchus labrax) behaviour as a function of manual and automatic feeding distribution modes. Under manual mode, the food is distributed over an extended area for a longer period, and its precise location is not always predictable, while with pneumatic automatic feeders, fish receive the same amount of resource, which is concentrated in the same surface area over a shorter period. We compared seabass behaviour under automatic and manual conditions collecting video image recordings before, during, and after feeding d…

Integrating functional traits into correlative species distribution models to investigate the vulnerability of marine human activities to climate change

Climate change and particularly warming are significantly impacting marine ecosystems and the services they provided. Temperature, as the main factor driving all biological processes, may influence ectotherms metabolism, thermal tolerance limits and distribution species patterns. The joining action of climate change and local stressors (including the increasing human marine use) may facilitate the spread of non-indigenous and native outbreak forming species, leading to associated economic consequences for marine coastal economies. Marine aquaculture is one among the most economic anthropogenic activities threatened by multiple stressors and in turn, by increasing hard artificial substrates …

The effect of fish farming organic waste on food availability for bivalve molluscs (Gaeta Gulf, Central Tyrrhenian, MED): stable carbon isotopic analysis

Stable carbon isotope (δ13C) analysis was used in a fish-farming impacted Mediterranean area (the Gulf of Gaeta, Central Tyrrhenian) to determine the predominant carbon sources available to bivalve molluscs cultivated around fish cages. Wether the organic matter generated by fish farming was taken up by the bivalve molluscs was also investigated. Stable carbon isotope values were measured in the particulate organic carbon (POC) of samples from potential organic matter sources such as fish-pelleted feed, mollusc faecal waste and bivalve flesh. The sources of organic matter affecting the study area water column and benthic communities appeared to be terrigenous-continental, autochthonous (phy…

Whistle variation in Mediterranean common bottlenose dolphin: The role of geographical, anthropogenic, social, and behavioral factors.

Abstract The studies on the variation of acoustic communication in different species have provided insight that genetics, geographic isolation, and adaptation to ecological and social conditions play important roles in the variability of acoustic signals. The dolphin whistles are communication signals that can vary significantly among and within populations. Although it is known that they are influenced by different environmental and social variables, the factors influencing the variation between populations have received scant attention. In the present study, we investigated the factors associated with the acoustic variability in the whistles of common bottlenose dolphin (Tursiops truncatu…

Predicting the current and future global distribution of the invasive freshwater hydrozoan Craspedacusta sowerbii

AbstractThe freshwater jellyfish Craspedacusta sowerbii is one of the most widespread invasive species, but its global distribution remains uncertain due to ephemeral appearances and general lack of information in various aquatic environments. The aim of this study was to map current and future distributions (2050 and 2100) using Species Distribution Models allowing to visualize the habitat suitability and make projections of its changes under potential climate change scenarios. Except in Oceania where the range decreased, an expansion of C. sowerbii was projected during the next century under modeled future scenarios being most intensive during the first half of the century. The present st…

Temperature modulates the response of the thermophilous sea urchin Arbacia lixula early life stages to CO2-driven acidification

The increasing abundances of the thermophilous black sea urchin Arbacia lixula in the Mediterranean Sea are attributed to the Western Mediterranean warming. However, few data are available on the potential impact of this warming on A. lixula in combination with other global stressors such as ocean acidification. The aim of this study is to investigate the interactive effects of increased temperature and of decreased pH on fertilization and early development of A. lixula. This was tested using a fully crossed design with four temperatures (20, 24, 26 and 27 °C) and two pH levels (pHNBS 8.2 and 7.9). Temperature and pH had no significant effect on fertilization and larval survival (2d) for te…

A new lessepsian species in the western Mediterranean ( Brachidontes pharaonis Bivalvia: Mytilidae): density, resource allocation and biomass

The present study reports on population dynamics and growth performance relative to a lesser known exotic invasive species ( Brachidontes pharaonis ) inhabiting the southern Mediterranean. The study was carried out in western Sicily, where B. pharaonis is present on both the submerged and emerged surfaces of a hyperhaline saltpan. Individuals were scraped, counted and measured for shell length, total weight, somatic, gonadic and shell biomass. Brachidontes pharaonis intensively colonized all hard substrates of the saltpan with annual average densities of 375 ± 293 ind. 400 cm −2 with density peaks in autumn as a function of habitat. The occurrence of juveniles was different for mediolittora…

Predicting patterns of stress and mortality in intertidal invertebrates: applications of biophysical ecology in a changing world

Abstract Background , Questions and Methods Recent studies have emphasized that local and geographic patterns of species distributions can be set by a variety of factors related to weather and climate, including exposure to lethal environmental conditions, indirect effects on consumers and competitors, and sublethal effects of physiological stress on growth and reproduction. Predicting where, when and with what magnitude these impacts are most (and least) likely to occur is imperative if we are to effectively plan for (i.e. adapt to) the effects of climate change.We developed a series of methods for translating patterns of environmental “signals” into organismal responses in intertidal ecos…

Testing the effects of temporal data resolution on predictions of the effects of climate change on bivalves

a b s t r a c t The spatial-temporal scales on which environmental observations are made can significantly affect our perceptions of ecological patterns in nature. Understanding potential mismatches between environmen- tal data used as inputs to predictive models, and the forecasts of ecological responses that these models generate are particularly difficult when predicting responses to climate change since the assumption of model stationarity in time cannot be tested. In the last four decades, increases in computational capacity (by a factor of a million), and the evolution of new modeling tools, have permitted a corresponding increase in model complexity, in the length of the simulations,…

Integrating multiple stressors in aquaculture to build the blue growth in a changing sea

Fisheries currently represent the main source of animal protein intake worldwide, although catches of most commercial species are at or beyond maximum sustainable yields. Increasing production would require an excess of exploitation levels and aquaculture is expected to become crucial in sustaining a growing seafood demand. Nonetheless, many threats are expected to affect aquaculture and the increased production must evolve in a way that minimizes environmental and socio-economic impacts. The claimed sustainable development of human activities at sea (blue growth and economy) seeks for new joint analyses and solutions at (trans-)national systemic level should be planned and applied. To meet…

Behavioral response of brown meagre (Sciaena umbra) to boat noise

Underwater man-made noise is recognized as a major global pollutant in the 21st Century, and its reduction has been included in national and international regulations. Despite the fact that many studies have pointed out the ecological impact of noise on marine organisms, few studies have investigated - in a field context - the behavioral response to boat noise in fish. In the present study we measure how Sciaena umbra reacts to boat noise. We found that boat noise: i) increased duration of flight reactions and number of individuals performing them, ii) increased the frequency of hiding behaviors, and iii) did not elicit a change in fish activity level and sound emission. Flights and hiding …

An assessment of regulation, education practices and socio-economic perceptions of non-native aquatic species in the Balkans

Alongside climate change, the introduction of non- native species (NNS) is widely recognized as one of the main threats to aquatic biodiversity and human wellbeing. Non-native species and biodiversity are generally low priority issues on the political agendas of many countries, particularly in European countries outside the European Union (EU). The objectives and tasks of this study were to address the policy regulation, education level, education practices, and socioeconomic perceptions of NNS in the Balkans. A questionnaire based survey was conducted in Albania, Bosnia and Herzegovina, Montenegro, North Macedonia and Turkey (Balkan EU candidate and potential candidate members), in Croatia…

Ecological effects of aquaculture on living and non-living suspended fractions of the water column: a meta-analysis.

The effects of aquaculture on the ecology of the water column have been extensively studied in the last two decades. However, to date, it has not been possible to extrapolate homogeneous information from the peer-reviewed literature. In the present study, 68 peer-reviewed articles were analysed and about 1087 study cases were used to test whether worldwide cultivations of aquatic organisms (shrimps, fish, bivalves and polyculture) have a differential effect on living and non-living fractions of the water column (suspended matter, chlorophyll-a, particulate organic carbon, nitrogen and phosphorus, bacteria and plankton). Meta-analysis feasibility depends on obtaining an estimate of the effec…

Predicting common bottlenose dolphin habitat preference to dynamically adapt management measures from a Marine Spatial Planning perspective

Abstract At the European Level, SACs (Special Areas of Conservation) are considered among the most reliable tools for increasing the efficiency of protective actions and to identify species vulnerability hotspots across spatial scales. Nevertheless, SACs may fail in their scope when design and management are not dynamically adapted to meet ecological principles. Knowledge of the spatial distribution of relevant key species, such as common bottlenose dolphin (Tursiops truncatus), is crucial in order to achieve the objective of the Habitat Directive (92/43/EEC), and is a fundamental step in the process of Marine Spatial Planning. From this perspective, new data and analysis are required to pr…

Eco-physiological response of two marine bivalves to acute exposition to commercial Bt-based pesticide

Microbial products based on the entomopathogenic bacterium Bacillus thuringiensis (Bt) are among the most common biopesticides used worldwide to suppress insect pests in forests, horticulture and agricultural crops. Some of the effects of commercial Bt have been recorded for terrestrial and freshwater non-target organisms but little research is available on marine fauna. Nevertheless, due to the contiguity of agro-ecosystems and coastal habitats, marine fauna may be highly influenced by this control method. We studied the effect of a commercial Bt product on the physiological and ecological responses and the energy budget of two of the most frequent marine intertidal bivalves in the Mediter…

Short-term exposure to concurrent biotic and abiotic stressors may impair farmed molluscs performance

Global warming, through increasing temperatures, may facilitate the spread and proliferation of outbreak-forming species which may find favourable substrate conditions on artificial aquaculture structures. The presence of stinging organisms (cnidarian hydroids) in the facilities fouling community are a source of pollution that can cause critical problems when in-situ underwater cleaning processes are performed. Multiple stressor experiments were carried out to investigate the cumulative effect on farmed mussels' functional traits when exposed to realistic stressful conditions, including presence of harmful cnidarian cells and environmental conditions of increasing temperature and short-term…

Assessing geographical variation on whistle acoustic structure of three Mediterranean populations of common bottlenose dolphin (Tursiops truncatus)

Whistles are acoustic signals produced particularly during social interactions. Here, we compare whistles by bottlenose dolphins from three Mediterranean areas (Croatia, Sicily and Sardinia) to investigate the presence of acoustic divergence and to discuss the possible causes of variability. Whistle parameters differ significantly between populations, but PCA highlights that the majority of variability is due to a limited number of frequency parameters. Cluster and DFA show that the Croatian population is acoustically divergent from the western populations of Sicily and Sardinia. This divergence could be consistent with geographical isolation, and a possible genetic differentiation between …

Influence of fish aggregating devices (FADs) on anti-predator behaviour within experimental mesocosms

Commercial fishers have used fish aggregating devices throughout the Mediterranean Sea for over 40 years. These devices attract numerous predatory and forage species in both coastal and offshore environments. This study examined the influence of fish aggregating devices on schooling and aggregating behaviour by small forage fish in quasi-natural mesocosms. Anti-predator behaviour was evaluated for juvenile Caranx crysos under a variety of treatment conditions. Results suggest that, in the absence of physical structure, C. crysos first respond to a predatory threat by forming a school. When a physical structure is present, however, C. crysos show an occasional tendency to aggregate near the …

The entangled multi-level responses of Mytilus galloprovincialis (Lamarck, 1819) to environmental stressors as detected by an integrated approach.

Abstract Anthropogenic pressure adds up and interacts with the effects of climate change with a varying magnitude and potential changes depend on species’ Life History (LH) traits, local environmental conditions and co-occurrence of several stressors. Stressors exert negative effects on marine biota when acting as a single factor, but the effects may be amplified when more than one stressor work in combination, producing interacting effects on biodiversity and ecosystem functioning. The impairment of individual functional traits (FT) leads to strong rebounds on LH traits and this may have ecological consequences. No studies actually relate FT and antioxidant enzymes to multiple environmenta…

Comparison of growth performance and biometric relationships in two reciprocal sturgeon hybrids reared in net cages (Sicily, Mediterranean)

This study was designed to investigate growth performance in two sturgeon hybrids reared in two quadrangular floating cages moored in an artificial pond, representing the first experience of sturgeon culture performed at Southern Mediterranean latitudes. The research was carried out from December 2000 to July 2001 and tested the growth performance, feeding parameters and biometric relationships. The sturgeons were randomly collected from the cages on a monthly basis, and total length, standard length (SL) and wet weight (WW) were recorded. The specific daily growth rate (SGRW) as somatic WW, food conversion ratio and condition factor (CF) were calculated. Biometric relationships, as linear …

In-Gel Assay to Evaluate Antioxidant Enzyme Response to Silver Nitrate and Silver Nanoparticles in Marine Bivalve Tissues

Silver is back in vogue today as this metal is used in the form of nanomaterials in numerous commercial products. We have developed in-gel electrophoretic techniques to measure the activity of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPX), and used the same techniques in combination with HSP70 Western blot analysis to evaluate the effects of nanomolar amounts of silver nitrate and 5 nm alkane-coated silver nanoparticles in tissues of the marine bivalve Mytilus galloprovincialis (Lam.) exposed for 28 days in mesocosms. Our results showed a negligible effect for nanosilver exposure and dose-dependent effects for the nitrate form.

A bioenergetics framework for integrating the effects of multiple stressors: Opening a 'black box' in climate change research

Climate change is already impacting marine ecosystems across a range of scales, from individual physiology, to changes in species interactions and community structure, and ultimately to patterns in geographic distribution. Predicting how marine ecosystems will respond to environmental change is a signifi cant challenge because vulnerability to climatic and non-climatic stressors is highly variable, and depends on an organism’s functional traits, tolerance to stressors, and the environment in which it lives. We present a mechanistic approach based on biophysical and dynamic energy budget models that integrates the cumulative effects of multiple environmental stressors (temperature and food) …

The Status of Coastal Benthic Ecosystems in the Mediterranean Sea: Evidence From Ecological Indicators

The Mediterranean Sea is subject to multiple human pressures increasingly threatening its unique biodiversity. Spatially explicit information on the ecological status of marine ecosystems is therefore key to an effective maritime spatial planning and management, and to help the achievement of environmental targets. Here, we summarized scientific data on the ecological status of a selection of marine ecosystems based on a set of ecological indicators in more than 700 sites of the Mediterranean Sea. For Posidonia oceanica seagrass beds, rocky intertidal fringe, and coastal soft bottoms, more than 70% of investigated sites exhibited good to high ecological conditions. In contrast, about two-th…

Environmental Constraints on Pathways of Organic Detritus in a Semi-enclosed Marine System (W-Mediterranean)

In order to assess seasonal and spatial changes in water-sediment interaction processes in a semi-enclosed marine system of Western Sicily (Marsala lagoon; W-Mediterranean), the biochemical composition of suspended and sediment organic matter was studied, during a one-year sampling period. The observed dynamic balance of resuspension vs. sedimentation processes and the macroalgal and vascular plant coverage appear major factors in affecting both amounts and biochemical composition of suspended and sedimentary organic matter and allowed us to identify two different sub-systems. The northern area, characterised by frequent wind-induced sediment resuspension events and by a scant vegetation, d…

Ecological implications of purple sea urchin (Heliocidaris crassispina, Agassiz, 1864) enhancement on the coastal benthic food web: evidence from stable isotope analysis

A responsible approach to marine stock enhancement is an effective approach to restore fishery resources. While the release strategy of target species has been well investigated, the impacts on local ecological equilibrium and habitat qualities have only been poorly considered. In the present study, we evaluated how the macro-benthic food web in Daya Bay was affected by purple sea urchin (Heliocidaris crassispina (Agassiz, 1864) stock enhancement using stable isotope analyses (δ13C and δ15N). Our results indicated that the distribution of local species and trophic diversity were influenced to a certain degree by release of purple sea urchins and changes in the feeding habit of the urchins w…

Energetics, Particle Capture and Growth Dynamics of Benthic Suspension Feeders

Marine benthic communities are dominated by suspension feeders, including those actively pumping water, passively encountering particles, or some combination of the two. The mechanisms by which particles are encountered and retained are now well known for a range of water flow conditions and organism morphologies. Recent research has attempted to quantify the energetic components of suspension feeding, including intake of particles, pumping rates, and metabolic costs of these activities. Energetic models depend strongly on environmental conditions, including temperature, flow speed, and food availability, for example. The effects of these variables have been combined for realistic scenarios…

Seasonal changes in size, sex-ratio and body condition of the damselfishChromis chromisin the central Mediterranean Sea

Chromis chromisis one of the most abundant fish species on the Mediterranean rocky shores. Acting as a by-pass of nutrients from the pelagic to the rocky littoral system, it plays an important role as a resource provider and has a relevant function in the community assemblage.Chromis chromishas many of the characteristics required of an indicator species (e.g. it is easy to recognize in the field, small sized, widespread and abundant). In this study we focused on individual size, sex-ratio and body condition in a damselfish population occurring in a central Mediterranean marine protected area. Fishing trials on a monthly basis (April–November) allowed us to assess seasonal changes in popula…

Combined effects of thermal conditions and food availability on thermal tolerance of the marine bivalve, Perna viridis

Abstract Organisms can mitigate the effects of long term variation in environmental conditions through acclimation, which involves changes in various physiological responses. To elucidate the possible effects of temperature and food concentrations on acclimation capacity, physiological responses of the mussel, Perna viridis, were measured after individuals were held for six weeks under varying temperatures and food availability. Warm-acclimated mussels experiencing higher food levels had significantly greater upper thermal limits than those maintained on lower food levels. In contrast, the upper thermal limits of cold-acclimated mussels were not affected by food levels. For warm-acclimated …

Growth and reproductive simulation of candidate shellfish species at fish cages in the Southern Mediterranean: Dynamic Energy Budget (DEB) modelling for integrated multi-trophic aquaculture

Abstract A Dynamic Energy Budget (DEB) model is used to simulate growth and reproduction of the shellfish Mytilus galloprovincialis and Crassostrea gigas in an integrated multi-trophic aquaculture (IMTA) farm scenario situated in the Southern Mediterranean (the Gulf of Castellammare, Sicily). We modelled the effect of primary production enrichment at fish cages on shellfish growth and life history traits using 4 years-hourly temperature data (01 January 2006–31 December 2009) at a depth of 1 m. Outputs of the DEB simulations were: the maximum theoretical total shell length of shellfish, the potential reproductive outputs and the mean annual von Bertalanffy growth rate. There was a mean incr…

Environmental Conditions along Tuna Larval Dispersion: Insights on the Spawning Habitat and Impact on Their Development Stages

Estimated larval backward trajectories of three Tuna species, namely, Atlantic Bluefin Tuna (Thunnus thynnus, Linnaeus, 1758), Bullet Tuna (Auxis Rochei, Risso, 1801) and Albacore Tuna (Thunnus alalunga, Bonnaterre, 1788) in the central Mediterranean Sea, were used to characterize their spawning habitats, and to assess the impact of changes due to the major environmental parameters (i.e., sea surface temperature and chlorophyll-a concentration) on larval development during their advection by surface currents. We assumed that the environmental variability experienced by larvae along their paths may have influenced their development, also affecting their survival. Our results showed that the …

Downscaling hydrodynamics features to depict causes of major productivity of Sicilian-Maltese area and implications for resource management

Abstract Chlorophyll- a ( CHL-a ) and sea surface temperature ( SST ) are generally accepted as proxies for water quality. They can be easily retrieved in a quasi-near real time mode through satellite remote sensing and, as such, they provide an overview of the water quality on a synoptic scale in open waters. Their distributions evolve in space and time in response to local and remote forcing, such as winds and currents, which however have much finer temporal and spatial scales than those resolvable by satellites in spite of recent advances in satellite remote-sensing techniques. Satellite data are often characterized by a moderate temporal resolution to adequately catch the actual sub-gri…

Valuing the unmarketable: an ecological approach to the externalities estimate in fishing activities

In a rapidly changing world, sustainability, if it can be said to exist at all, is concept that has attained mythic status, often pursued and rarely reached. In order to improve our capability to cope with environmental problems, adopting an Ecosystem Approach has been suggested. One of the major challenges in the implementation of this new paradigm relates to control of externalities. The recognition and quantification of externalities is often cast as valuing the unmarketable, and there are several approaches that have been proposed. Here, we analyze the opportunity to “feed” the economic valuation with ecological concepts. From an ecological perspective, the energy required to sustain a …

Immediate biomarker responses to benzo[a]pyrene in polluted and unpolluted populations of the blue mussel (Mytilus edulis L.) at high-latitudes.

Immediate biomarker responses of two high-latitude populations of the blue mussel Mytilus edulis to benzo[a]pyrene (B[a]P) were evaluated. Mussels collected from a clean and a polluted site in southwest Iceland were exposed to the nominal dose of 100 μg B[a]P L−l for 3 h, after 4 days of acclimatization in clean seawater. To test the sensitivity to the toxicant and immediate biological responses, the following biomarkers were used: DNA single strand breaks, heart rate and feeding rate.All the biomarkers revealed differences between the study sites. Irrespective of the origin of the organisms, the short time exposure to the high B[a]P concentration did not induce DNA single strand breaks or …

Mediterranean rocky reefs in the Anthropocene: Present status and future concerns

Abstract Global change is striking harder and faster in the Mediterranean Sea than elsewhere, where high levels of human pressure and proneness to climate change interact in modifying the structure and disrupting regulative mechanisms of marine ecosystems. Rocky reefs are particularly exposed to such environmental changes with ongoing trends of degradation being impressive. Due to the variety of habitat types and associated marine biodiversity, rocky reefs are critical for the functioning of marine ecosystems, and their decline could profoundly affect the provision of essential goods and services which human populations in coastal areas rely upon. Here, we provide an up-to-date overview of …

Estimation of fitness from energetics and life-history data: An example using mussels.

Changing environments have the potential to alter the fitness of organisms through effects on components of fitness such as energy acquisition, metabolic cost, growth rate, survivorship, and reproductive output. Organisms, on the other hand, can alter aspects of their physiology and life histories through phenotypic plasticity as well as through genetic change in populations (selection). Researchers examining the effects of environmental variables frequently concentrate on individual components of fitness, although methods exist to combine these into a population level estimate of average fitness, as the per capita rate of population growth for a set of identical individuals with a particul…

An energy budget for the subtidal bivalve Modiolus barbatus (Mollusca) at different temperatures

Clearance rates, respiration rates and food absorption efficiencies of the commercially interesting subtidal bivalve Modiolus barbatus were measured at different temperatures under laboratory conditions and scope for growth calculated. Clearance rates were highest at temperatures from 20 °C to 28 °C, whereas respiration rate was maximal at 9 °C and minimal at 26 °C. Highest mean values of absorbed energy occurred at 20 °C and 26 °C. Scope for growth trend had negative values at 9 °C, 15 °C and 28 °C and positive values at temperatures 20 °C and 26 °C. The profitable thermal window for M. barbatus to have energy sufficient for growth and reproduction corresponded to <5 months per year. Seawa…

Response of captive seabass and seabream as behavioural indicator in aquaculture

Welfare of cultivate fish at high-density represents an important concern for modern aquaculture. The behaviour of European seabass (Dicentrarchus labrax) and seabream (Sparus aurata) reared in cages was studied in a fish farm of northern Sardinia (Italy) in autumn 2006 to test whether captive condition had an effect on the movement patterns of these two species.Video images recorded before, during and after the manual feeding distribution allowed us to collect data on different behaviours of captive fish. Thus, behaviours indicating the position of fish in the water column, swimming direction and possible aggressive behaviours (aggression, direction change and collision) showed juveniles a…

Scope For Growth of Mytilus galloprovincialis (Lmk., 1819) in oligotrophic coastal waters (southern Tyrrhenian Sea, Italy)

The ‘scope for growth’ (SFG) tool was used to study the growth performance of cultivated populations of Mytilus galloprovincialis (Lmk., 1819) in an oligotrophic area of the Southern Mediterranean Sea. The study was carried out between 1993 and 1996 by using data from four seasonal oceanographic cruises and from growth experiments. Water samples were collected and analysed for total suspended matter (TSM), particulate organic carbon (POC) and nitrogen (PON), particulate lipids, proteins and carbohydrates and chloropigments. The sum of the carbon equivalents of carbohydrates, proteins and lipids is indicated as the total biopolymeric particulate organic carbon (BPC) and was converted into a …

Measuring the effects of temperature rise on Mediterranean shellfish aquaculture

Abstract Shellfish aquaculture represents a worldwide valuable segment of the aquaculture market, spreading along the Mediterranean coasts, and is sensitive to the still unforeseen, poorly-known effects of climate change. Threats due to temperature rise can threaten the deployment and development of this sector, up until now recognised as the best candidate to mitigate the effects of fishery overexploitation. Here, we investigate the effects of temperature increase on the model species, Mytilus galloprovincialis, measuring outcomes from valve fragility (thickness) and condition index. Evidence of a reduction in the thickness of valves and the modulation condition of the mussels along with t…

Common Bottlenose Dolphin Protection and Sustainable Boating: Species Distribution Modeling for Effective Coastal Planning

Indicators for preserving marine biodiversity include knowledge of how the spatial distribution and critical habitats of species overlap with human activities and impacts. Such indicators are key tools for marine spatial planning, a process that identifies and resolves conflicts between human uses and the conservation of marine environments. The common bottlenose dolphin in the Mediterranean Sea is considered a vulnerable species by the IUCN Red List and a priority species of the EU Habitat Directive. Here, we estimated spatio-temporal patterns of the species occurrence in the area around one Marine Protected Area (MPA) and two Sites of Community Importance (SCI) of the North western Sardin…

Functional consequences of prey acclimation to ocean acidification for the prey and its predator

Ocean acidification is the suite of chemical changes to the carbonate system of seawater as a consequence of anthropogenic carbon dioxide (CO2) emissions. Despite a growing body of evidences demonstrating the negative effects of ocean acidification on marine species, the consequences at the ecosystem level are still unclear. One factor limiting our ability to upscale from species to ecosystem is the poor mechanistic understanding of the functional consequences of the observed effects on organisms. This is particularly true in the context of species interactions. The aim of this work was to investigate the functional consequence of the exposure of a prey (the mussel Brachidontes pharaonis) t…

Mapping the global distribution of the freshwater hydrozoan Craspedacusta sowerbii

The invasive freshwater jellyfish Craspedacusta sowerbii (Limnomedusae, Olindiidae) is native to East Asia and since the end of the 19th century, was observed in Europe, then in North America, and across the globe. In recent decades, reports of C. sowerbii have drastically increased in Europe, North and South America, Australia, Asia, and parts of Africa. However, the worldwide distribution of C. sowerbii remains poorly documented due to the lack of information in various aquatic environments. This dataset globalises the occurrences of this species from an extensive literature review and database review. Information extracted from the literature/database were organised and synthesised accor…