6533b86efe1ef96bd12cc04c
RESEARCH PRODUCT
An indirect immunofluorescent antibody technique for detection and enumeration of Vibrio vulnificus serovar E (biotype 2): delevopment and applications
Ester Marco-noalesCarmen AmaroElena G. BioscaMiguel Milánsubject
SerotypeVibrio vulnificusImmunofluorescenceSensitivity and SpecificityApplied Microbiology and BiotechnologyMicrobiologyFish DiseasesVibrionaceaeVibrio InfectionsEnumerationmedicineAnimalsSeawaterFluorescent Antibody Technique IndirectVibrioEelsbiologymedicine.diagnostic_testfungiGeneral Medicinebacterial infections and mycosesbiology.organism_classificationAntibodies BacterialVibrioVibrio InfectionsWater MicrobiologyBacteriaBiotechnologydescription
The applications of an indirect fluorescent antibody technique (IFAT), developed to detect and enumerate the pathogenic bacterium Vibrio vulnificus serovar E from water and clinical samples, are described. This technique proved accurate for detecting V. vulnificus, even under starvation conditions and in the non-culturable state, and could differentiate this species from other bacteria which share the same habitats. The IFAT was successfully used to diagnose vibriosis from naturally- and artificially-infected eels. The overall data suggest that applying this technique properly in environmental and epidemiological/epizootiological studies could significantly increase our knowledge of this bacterium.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-10-01 | Journal of Applied Microbiology |