6533b873fe1ef96bd12d4dfc

RESEARCH PRODUCT

Dynamic electric field assisted multi-dimensional liquid chromatography of biological samples

M. QuagliaBrian A. GrimesDieter LubdaO. KornyšovaK.k. UngerT.p. Hennessy

subject

Polarity reversalAnalyteChromatographyChemistryClinical BiochemistrySize-exclusion chromatographyAnalytical chemistryCell BiologyGeneral MedicineReversed-phase chromatographyChromatography Ion ExchangeSensitivity and SpecificityBiochemistryAnalytical ChemistryMatrix (chemical analysis)Electric fieldPotential gradientChromatography GelHumansElectrophoresis Polyacrylamide GelElectric potentialBlood Chemical Analysis

description

Complex biological samples require very high resolution separation strategies. The platform introduced here capitalises on the hyphenation of liquid chromatographic (LC) and electric potential gradient electrochromatographic multi-dimensional separation genres. First-dimension selectivity is provided by simultaneous size exclusion (SEC) and strong cation exchange (SCX) chromatography modes, while the second dimension comprises reversed phase (RP) characteristics in a dynamic (time-variant) electric field. The time-variant potential gradient with reversal of polarity is applied across the second dimension monolithic capillary throughout the duration of the solvent strength gradient elution. Hence, the platform offers comprehensive on-line sample clean-up (matrix depletion, analyte enrichment), fractionation (first dimention LC), and separation (second dimension LC) with the prospect of altering selectivity via polarity reversal dynamic electric field tuning.

yearjournalcountryeditionlanguage
2005-03-05Journal of Chromatography B
https://doi.org/10.1016/j.jchromb.2004.10.043