Search results for " Albumin"
showing 10 items of 320 documents
Validation of glycated albumin reference interval in healthy Caucasian pregnant women
2022
No abstract available
GALACTOSE-DECORATED POLYMERIC CARRIERS FOR HEPATOCYTE-SELECTIVE DRUG TARGETING
2015
In this paper, the current available strategies to realize galactose-decorated nanostructured polymeric systems are summarized. These carriers are designed in order to obtain targeted drug delivery to hepatocytes via galactose (GAL) moieties, i.e., for the treatment of viral hepatitis or liver cancer that are the greater causes of global disability and mortality. Usually, the main followed strategy to obtain galactosylated polymeric carriers is to use galactosylated copolymers. The chemical modifications of preformed polymers with sugar-containing reagents is followed for obtaining lactosaminated human albumin, galactosylated phospholipid-polyaminoacid and polylactide (PLA)- polyaminoacid c…
Prognostic value of IL-6 and IL-10 serum levels and immunonutritional assessment in determining postoperative complications after geriatric surgery
2009
The onset of postoperative septic complications has recently been associated to serum levels of interleukin (IL) IL-6 and IL-10. Patients with complications have higher serum levels than patients without complication after surgery. IL-6 has been recognized as early marker of postoperative septic events. Furthermore IL-6 increases with age, expecially if there are associated diseases. It is a sign of functional deterioration of different organs. The disregulation in interleukin production, anesthetic drugs, hemorrhage and transfusions may lead to the onset of postoperative complications. The latter are more frequent in oncologic patients. The aim of our study is to evaluate pre- and postoper…
Protein delivery based on uncoated and chitosan-coated mesoporous silicon microparticles
2011
Mesoporous silicon is a biocompatible, biodegradable material that is receiving increased attention for pharmaceutical applications due to its extensive specific surface. This feature enables to load a variety of drugs in mesoporous silicon devices by simple adsorption-based procedures. In this work, we have addressed the fabrication and characterization of two new mesoporous silicon devices prepared by electrochemistry and intended for protein delivery, namely: (i) mesoporous silicon microparticles and (ii) chitosan-coated mesoporous silicon microparticles. Both carriers were investigated for their capacity to load a therapeutic protein (insulin) and a model antigen (bovine serum albumin) …
Isolation of human milk whey proteins by solid phase extraction with a polymeric material modified with gold nanoparticles
2017
Abstract This work describes a method for the isolation of human milk whey proteins by solid-phase extraction (SPE) with a polymeric material modified with gold nanoparticles. Human serum albumin, α-lactalbumin, lactoferrin and lysozyme were selected as target proteins to establish the performance of SPE support. Several experimental variables (pH and ionic strength) that affect the SPE protocol were investigated to achieve the maximum extraction efficiency. Under optimal conditions, the SPE sorbent gave excellent recoveries, and offered a high permeability and reusability (> 20 times). The feasibility of this methodology was successfully demonstrated by isolating the target proteins in a m…
High-performance liquid chromatography of amino acids, peptides and proteins
1990
Abstract The thermodynamic constants, associated with the interaction of three proteins with triazine dye affinity sorbents, have been derived from bath and frontal analysis experiments. In cases where mass-transfer restrictions are very high, calculation of the thermodynamic constants directly from frontal analysis experiments could not be achieved. In such cases, a portion of the adsorbate was always present in the effluent, a situation which has its effect as the split peak phenomenon. With Fractogel-based triazine dye affinity sorbents none of the test proteins applied in frontal analysis were adsorbed. A similar behaviour was observed for a Cellufine sorbent during the adsorption of hu…
Solid-phase extraction based on ground methacrylate monolith modified with gold nanoparticles for isolation of proteins
2015
In this study, a novel polymeric material functionalized with gold nanoparticles (AuNPs) was prepared as solid-phase extraction (SPE) sorbent for isolation of proteins. The sorbent was synthesized from a powdered poly(glycidyl-co-ethylene dimethacrylate) monolith, and modified with ammonia, followed by immobilization of AuNPs on the pore surface of the material. To evaluate the performance of this SPE support, proteins were selected as test solutes, being the extraction conditions and other parameters (loading capacity and regenerative ability of sorbent) established. The results indicated that this sorbent could be employed to selectively capture proteins according to their pI, on the basi…
Synthetic Haptens and Monoclonal Antibodies to the Cyanotoxin Anatoxin‐a
2019
Early warning systems for monitoring toxic events may benefit from the availability of monoclonal antibodies enabling the sensitive and specific detection of anatoxin-a, a cyanotoxin involved in numerous cases of animal poisoning resulting from toxic algal blooms in freshwaters. Through the synthesis of three functionalized derivatives of anatoxin-a, we have succeeded in generating the first-ever reported immunoreagents (bioconjugates and antibodies) suitable for the development of immunoanalytical approaches aimed at rapid and onsite detection of this harmful cyanotoxin.
Albumin-driven disassembly of lipidic nanoparticles: the specific case of the squalene-adenosine nanodrug
2020
International audience; In the field of nanomedicine, nanostructured nanoparticles (NPs) made of self-assembling prodrugs emerged in the recent years with promising properties. In particular, squalene-based drug nanoparticles have already shown their efficiency through in vivo experiments. However, a complete pattern of their stability and interactions in the blood stream is still lacking. In this work we assess the behavior of squalene-adenosine (SQAd) nanoparticles-whose neuroprotective effect has already been demonstrated in murine models-in the presence of fetal bovine serum (FBS) and of bovine serum albumin (BSA), the main protein of blood plasma. Extensive physicochemical characteriza…
Low density lipoproteins and human serum albumin as the carriers of squalenoylated drugs: insights from molecular simulations
2018
We have studied the interaction of three clinically promising squalenoylated drugs (gemcitabine-squalene, adenine-squalene, and doxorubicin-squalene) with low-density lipoproteins (LDL) by means of atomistic molecular dynamics simulations. It is shown that all studied squalenoylated drugs accumulate inside the LDL particles. This effect is promoted by the squalene moiety, which acts as an anchor and drives the hydrophilic drugs into the hydrophobic core of the LDL lipid droplet. Our data suggest that LDL particles could be a universal carriers of squalenoylated drugs in the bloodstream. Interaction of gemcitabine-squalene with human serum albumin (HSA) was also studied by ensemble of dockin…