Search results for " EVALUATION"

Synthesis and biological evaluation of antimalarial and antileukemic activity of new C-10 modified artemisinin derivatives

2021

Abstract Several non-acetal artemisinin derivatives were synthesized and their antimalarial activity against chloroquine-resistant parasites as well as their antileukemic activity were investigated. The azide 10 was proved to have slightly better activity than the current antimalarial drugs such as artesunate and chloroquine. In addition, the azide 10 and the aldehyde 7 were found to be effective in decreasing cell proliferation in leukemia cells.

Application of Imprinted Synthetic Polymers in Binding Assay Development

2000

The first part of the review describes a method for the synthesis of molecularly imprinted polymers for use in binding assays. The method considers the many factors involved that affect the recognition properties of the materials and describes an approach to screening and optimization of these factors. The second part describes the development of binding assays using such polymers. This includes the use of different labels, the effect of solvent and buffer, the scale of the assay (amount of solid polymer), and how these influence the quality of the assay in terms of sensitivity, selectivity, and speed of analysis.

Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes.

2012

Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for a multiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. the most relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.

Determination of Plasma Lipid Hydroperoxides by an NADPH/NADP + Coupled Enzyme Reaction System. Evaluation of a Method

1998

Summary: Several techniques based on different principles have been proposed to measure lipid hydroperoxides. Enzymatic methods are sensitive and can be quite specific but they are subject to interference by inhibitors and not all are stoichiometric. The present work proposes some modifications of the Heath & Tappel (Anal Biochem 1976; 7:184—91) enzymatic method of determination of lipid hydroperoxides in order to standardize and automate it and to meet the analytical criteria required for a biological assay. The proposed new protocol and the automated assay give acceptable within-run and between-run precisions, with coefficients of variation of 3.34% and 5.80%, respectively, at the usual p…

Design, synthesis, biological evaluation and molecular modelling of substituted pyrrolo[2,1-a]isoquinolinone derivatives: discovery of potent inhibit…

2021

We report here the design, synthesis and biological evaluation of a new series of substituted pyrrolo[2,1-a]isoquinolin-3-one derivatives, some of which have strong inhibitory activity against both AChE and BChE enzymes. The design of these new inhibitors was carried out taking rivastigmine as the starting structure. Thus, on the basis of an exhausting molecular modeling study using combined techniques (docking, dynamic molecular simulations and QTAIM calculations), we obtained new ligands possessing stronger inhibitory effects than rivastigmine, the reference compound. QTAIM analysis gave us detailed information about the molecular interactions stabilizing the different ligand–enzyme compl…

Conducting sensory analysis tests among the elderly

2015

A cognitive/decision-making model of evaluation for ethical urban and building renewal

2009

Creativity is a strategic factor not only in the production sector, but also for planning, development and management of the city. The physical shape of the city is determined by the plan of buildings and settlements as a whole. Nevertheless, the functional and aesthetical criteria alone are inadequate, if you adopt the view of sustainability. In particular, from biological, ecological and economic points of view, the shape of the sustainable city is the result of a process of creative choice of traditional and innovative forms, materials and technologies that contribute to the quality of buildings and urban ambient. This principle applies a fortiori in cities interested by not-negligible p…

Les théoriciens spontanés : comment les évaluateurs construisent et révisent leurs connaissances des programmes par l'expérience

2021

International audience; What exactly do evaluators learn about programs through experience and how? We chose a constructivist framework to investigate the structure of evaluators’ program-related knowledge, namely the form, content, and origins of their expressed theories. In this context, we complemented a Piagetian theory of learning with new developments offered by “probabilistic” (or “Bayesian”) models of learning. We conducted “explicitation” interviews with nine experienced practitioners, some specializing in the education or agri-environment sectors and some generalists. After examining the form and content of their program-related knowledge, we examine its development through experi…

Dagli obiettivi educativi alle competenze fondamentali

2014

Insegnare e valutare per competenze costituisce la "nuova frontiera" della Didattica contemporanea. Il libro si colloca nella prospettiva della scuola delle competenze senza però disperdere quanto di positivo è stato acquisito nella pratica della didattica per obiettivi di "terza generazione", quella che fa riferimento al sistema degli obiettivi educativi "fondamentali". Si ritiene che le critiche alla progettazione e alla valutazione per obiettivi abbiano contribuito a diffondere una concezione più ampia degli obiettivi educativi, che ben si integra con la nuova prospettiva didattica delle competenze. Il sistema degli obiettivi fondamentali dell'educazione, descritto nel volume, costituisc…

Cross-Device Augmented Reality Annotations Method for Asynchronous Collaboration in Unprepared Environments

2021

Augmented Reality (AR) annotations are a powerful way of communication when collaborators cannot be present at the same time in a given environment. However, this situation presents several challenges, for example: how to record the AR annotations for later consumption, how to align virtual and real world in unprepared environments or how to offer the annotations to users with different AR devices. In this paper we present a cross-device AR annotation method that allows users to create and display annotations asynchronously in environments without the need for prior preparation (AR markers, point cloud capture, etc.). This is achieved through an easy user-assisted calibration process and a …