Search results for " Humans"

showing 10 items of 2466 documents

Convergence of Wnt signaling on the HNF4alpha-driven transcription in controlling liver zonation.

2009

Background & Aims: In each hepatocyte, the specific repertoire of gene expression is influenced by its exact location along the portocentrovenular axis of the hepatic lobule and provides a reason for the liver functions compartmentalization defined "metabolic zonation." So far, few molecular players controlling genetic programs of periportal (PP) and perivenular (PV) hepatocytes have been identified; the elucidation of zonation mechanisms remains a challenge for experimental hepatology. Recently, a key role in induction and maintenance of the hepatocyte heterogeneity has been ascribed to Wnt/β-catenin pathway. We sought to clarify how this wide-ranging stimulus integrates with hepatocyte s…

Beta-cateninWnt ProteinCellular differentiationBlotting WesternLiver Stem CellFluorescent Antibody TechniqueMice TransgenicBiologyTransfectionSensitivity and SpecificityAnimals; Blotting Western; Cell Differentiation; Cell Proliferation; Cells Cultured; Fluorescent Antibody Technique; Hepatocyte Nuclear Factor 4; Hepatocytes; Humans; Immunoprecipitation; Mice; Mice Knockout; Mice Transgenic; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity; Signal Transduction; Transfection; Wnt Proteins; beta Catenin; GastroenterologyMiceliver zonation; wnt signalling; beta catenin; hnf4Gene expressionmedicineAnimalsHumansImmunoprecipitationHepatocyteCells Culturedbeta CateninCell ProliferationMice KnockoutHepatologyAnimalReverse Transcriptase Polymerase Chain ReactionGastroenterologyWnt signaling pathwayCell DifferentiationMolecular biologyWnt Proteinsmedicine.anatomical_structureHepatocyte nuclear factor 4Hepatocyte Nuclear Factor 4Hepatocytebiology.proteinHepatocytesChromatin immunoprecipitationHumanSignal TransductionGastroenterology
researchProduct

Essential versus accessory aspects of cell death: recommendations of the NCCD 2015

2015

Cells exposed to extreme physicochemical or mechanical stimuli die in an uncontrollable manner, as a result of their immediate structural breakdown. Such an unavoidable variant of cellular demise is generally referred to as ?accidental cell death' (ACD). In most settings, however, cell death is initiated by a genetically encoded apparatus, correlating with the fact that its course can be altered by pharmacologic or genetic interventions. "Regulated cell death" (RCD) can occur as part of physiologic programs or can be activated once adaptive responses to perturbations of the extracellular or intracellular microenvironment fail. The biochemical phenomena that accompany RCD may be harnessed to…

Biochemical Manifestations of Cell DeathISCHEMIA-REPERFUSION INJURYApoptosisReviewTransduction (genetics)0302 clinical medicineCASPASE INHIBITION SWITCHESAnimals; Humans; Terminology as Topic; Apoptosis; Signal Transduction610 Medicine & healthCaspaseTUMOR-NECROSIS-FACTOR0303 health sciencesSettore BIO/17biologySettore BIO/11NeurodegenerationSettore BIO/13APOPTOSIS3. Good healthMedicina Básicacell death030220 oncology & carcinogenesiscell death; Morphologic Aspects of Cell Death; Biochemical Manifestations of Cell DeathSignal transductionDOMAIN-LIKE PROTEINIntracellularHumanSignal TransductionNecroptosiCYTOCHROME-C RELEASEOUTER-MEMBRANE PERMEABILIZATIONProgrammed cell deathCIENCIAS MÉDICAS Y DE LA SALUDSettore BIO/06Inmunología610 Medicine & healthCELL DEATHNOQ-VD-OPH03 medical and health sciencesSettore MED/04 - PATOLOGIA GENERALEddc:570Terminology as TopicAPOPTOSIS-INDUCING FACTORMIXED LINEAGE KINASEmedicineAnimalsHumansAnimals; Humans; Terminology as Topic; Apoptosis; Signal Transduction; Molecular Biology; Cell BiologyMorphologic Aspects of Cell DeathSettore BIO/10Molecular Biology030304 developmental biologyAnimalCell growthApoptosiBiology and Life SciencesCell Biologymedicine.diseaseMITOCHONDRIAL PERMEABILITY TRANSITIONApoptosisImmunologybiology.proteinNeuroscienceCell death and differentiation
researchProduct

10-Year Clinical Experience With 18F-Choline PET/CT: An Italian Multicenter Retrospective Assessment of 3343 Patients.

2020

Purpose The primary aim of this multicenter retrospective analysis is to examine the role of(18)F-choline PET/CT as a diagnostic tool for staging and restaging prostate cancer (PCa) in a large population in the light of 10 years of clinical experience. A secondary aim of the study is to produce data on the predictors of a positive(18)F-choline PET/CT result in the setting of PCa primaries and biochemical recurrences. Materials and Methods This multicenter retrospective cohort study is based on data collected by 9 Italian nuclear medicine departments. Between October 2008 and September 2019, 3343 men underwent(18)F-choline PET/CT scans before receiving definitive treatments for a primary PCa…

Biochemical recurrenceMalemedicine.medical_specialtyProstate biopsyrecurrencePET/CTcholine; PET/CT; prostate cancer; recurrence; staging030218 nuclear medicine & medical imagingCholine03 medical and health sciencesProstate cancer0302 clinical medicineSettore MED/36ProstatePositron Emission Tomography Computed Tomography80 and overMedicineHumansRadiology Nuclear Medicine and imagingTomographyCancer stagingAgedAged 80 and overPET-CTmedicine.diagnostic_testbusiness.industryProstatic NeoplasmsRetrospective cohort studyGeneral MedicinestagingMiddle Agedmedicine.diseaseprostate cancerX-Ray ComputedProstate-specific antigenmedicine.anatomical_structurePET030220 oncology & carcinogenesisRadiologyNeoplasm GradingRadiopharmaceuticalsbusinessSettore MED/36 - Diagnostica Per Immagini E RadioterapiaTomography X-Ray ComputedCT; choline; prostate cancer; recurrence; staging; Aged; Aged 80 and over; Choline; Humans; Male; Middle Aged; Neoplasm Grading; Positron Emission Tomography Computed Tomography; Prostatic Neoplasms; Radiopharmaceuticals; Tomography X-Ray ComputedCTClinical nuclear medicine
researchProduct

The Sixth Annual Translational Stem Cell Research Conference of the New York Stem Cell Foundation

2012

The New York Stem Cell Foundation's "Sixth Annual Translational Stem Cell Research Conference" convened on October 11-12, 2011 at the Rockefeller University in New York City. Over 450 scientists, patient advocates, and stem cell research supporters from 14 countries registered for the conference. In addition to poster and platform presentations, the conference featured panels entitled "Road to the Clinic" and "The Future of Regenerative Medicine". © 2012 New York Academy of Sciences.

Biochemistry Genetics and Molecular Biology (all)Muscular DiseaseDiabetes MellituHematopoietic Stem CellRegenerative MedicineStem Cell ResearchHeart DiseaseHistory and Philosophy of ScienceDiabetes Mellitus; Heart Diseases; Hematopoietic Stem Cells; Humans; Muscular Diseases; Neoplasms; Nervous System Diseases; Regenerative Medicine; Translational Medical Research; Stem Cell Research; Stem Cell Transplantation; Biochemistry Genetics and Molecular Biology (all); History and Philosophy of ScienceNeoplasmNervous System DiseaseTranslational Medical ResearchHumanStem Cell Transplantation
researchProduct

In vivo biodistribution and lifetime analysis of cy5.5-conjugated rituximab in mice bearing lymphoid tumor xenograft using time-domain near-infrared …

2008

Rituximab is a chimeric monoclonal antibody directed against human CD20 antigen, which is expressed on B-cell lymphocytes and on the majority of B-cell lymphoid malignancies. Herein we report the conjugate of rituximab with the near-infrared (NIR) fluorophore Cy5.5 (RI-Cy5.5) as a tool for in vitro, in vivo, and ex vivo NIR time-domain (TD) optical imaging. In vitro, RI-Cy5.5 retained biologic activity and led to elevated cell-associated fluorescence on tumor cells. In vivo, TD optical imaging analysis of RI-Cy5.5 injected into lymphoma-bearing mice revealed a slow tumor uptake and a specific long-lasting persistence of the probe within the tumor. Biodistribution studies after intraperiton…

BiodistributionPathologymedicine.medical_specialtylcsh:Medical technologyLymphomamedicine.medical_treatmentIntraperitoneal injectionTransplantation HeterologousBiomedical EngineeringCarbocyanineMice SCIDBiologyIntestinal absorptionAntibodies Monoclonal Murine-DerivedMiceIn vivomedicineAnimalsHumansRadiology Nuclear Medicine and imagingAnimals; Antibodies Monoclonal; Antibodies Monoclonal Murine-Derived; Binding Sites; Carbocyanines; Cell Division; Female; Humans; Immunohistochemistry; Intestinal Absorption; Lymph Nodes; Lymphoma; Mice; Mice SCID; Neoplasm Transplantation; Rituximab; Transplantation Heterologouslcsh:QH301-705.5Binding SitesAnimaltechnology industry and agricultureBinding SiteAntibodies MonoclonalLymph NodeCarbocyaninesCondensed Matter PhysicsImmunohistochemistryTransplantationlcsh:Biology (General)lcsh:R855-855.5Intestinal AbsorptionMonoclonalMolecular MedicineImmunohistochemistryFemaleLymph NodesRituximabEx vivoCell DivisionNeoplasm TransplantationBiotechnologyHuman
researchProduct

The value of open-source clinical science in pandemic response

2021

International audience

Biomedical Research[SDV]Life Sciences [q-bio]Clinical scienceGlobal HealthCorrectionsMicrobiology1117 Public Health and Health Services1108 Medical MicrobiologyPandemicEconomicsHumansISARIC Clinical Characterisation GroupPandemicsCOVID-19/epidemiologyActuarial scienceInformation DisseminationSARS-CoV-2CommentISARICCOVID-191103 Clinical SciencesCommunicable Disease Control/methodsInfectious DiseasesOpen sourceCommunicable Disease ControlCOVID-19; Communicable Disease Control; Global Health; Humans; SARS-CoV-2; Biomedical Research; Information Dissemination; PandemicsValue (mathematics)HumanThe Lancet Infectious Diseases
researchProduct

Hysteroscopic Evaluation of Endometrial Changes in Breast Cancer Women with or without Hormone Therapies: Results from a Large Multicenter Cohort Stu…

2020

ABSTRACT Study Objective The primary aim of our study was to investigate the incidence of endometrial pathologies, especially endometrial cancer, in women with breast cancer treated with tamoxifen (TAM), aromatase inhibitors (AIs), or receiving no treatment (NT). The secondary aim was to identify, in this cohort, ultrasonographic findings that represent robust indications for hysteroscopy and endometrial biopsy, to avoid unnecessary second-level diagnostic procedures. Design Multicenter retrospective cohort study (Clinical Trial ID: NCT03898947). Setting Data were collected from different Italian centers: Regina Elena National Cancer Institute of Rome, Arbor Vitae Centre of Rome, Gaetano Ma…

BiopsyAromatase inhibitors Breast cancer Endometrial cancer Endometrial pathologies Tamoxifen Adult Aged Aged 80 and over Antineoplastic Agents Hormonal Biopsy Breast Neoplasms Cohort Studies Endometrial Hyperplasia Endometrial Neoplasms Endometrium Female Humans Hysteroscopy Incidence Middle Aged Polyps Precancerous Conditions Pregnancy Retrospective Studies Tamoxifen Uterine Diseases Uterine NeoplasmsCohort StudiesEndometrium0302 clinical medicineBreast cancerEndometrial cancerPregnancyAromatase inhibitors; Breast cancer; Endometrial cancer; Endometrial pathologies; TamoxifenAged 80 and overUterine Diseases030219 obstetrics & reproductive medicinemedicine.diagnostic_testEndometrial pathologiesIncidenceObstetrics and GynecologyMiddle AgedAromatase inhibitorsHysteroscopy030220 oncology & carcinogenesisEndometrial HyperplasiaUterine NeoplasmsFemalemedicine.drugAromatase inhibitors Breast cancer Endometrial cancer Endometrial pathologies TamoxifenAdultmedicine.medical_specialtyAntineoplastic Agents HormonalBreast NeoplasmsHysteroscopy03 medical and health sciencesBreast cancerPolypsmedicineEndometrial PolypHumansAgedRetrospective StudiesGynecologybusiness.industryEndometrial cancerCancerRetrospective cohort studymedicine.diseaseEndometrial NeoplasmsTamoxifenbusinessPrecancerous ConditionsTamoxifenEndometrial biopsy
researchProduct

Quality of life following urinary diversion: Orthotopic ileal neobladder versus ileal conduit. A multicentre study among long-term, female bladder ca…

2019

Abstract Introduction Women undergoing radical cystectomy (RC) followed by urinary diversion (UD) for bladder cancer experience a substantial reduction in health-related quality of life (HRQOL). At present, studies comparing long-term QOL outcomes for different UD methods, needed to inform evidence-based choices of bladder reconstruction for female patients, are sparse. Our objective was to compare two common UD methods in terms of their HRQOL outcomes in women. Materials and methods We retrospectively analysed HRQOL in 73 consecutive female bladder cancer patients having undergone orthotopic ileal neobladder (IONB, N = 24) or ileal conduit (IC, N = 49) following RC between 2007 and 2013 in…

Bladder cancer; Ileal conduit; Orthotopic neobladder; Quality of life; Radical cystectomy; Women;Time Factorsmedicine.medical_treatment030232 urology & nephrologyUrinary DiversionBladder cancer; Ileal conduit; Orthotopic neobladder; Quality of life; Radical cystectomy; Women; Adult; Aged; Aged 80 and over; Cancer Survivors; Cross-Sectional Studies; Female; Follow-Up Studies; Humans; Ileum; Italy; Middle Aged; Surveys and Questionnaires; Survival Rate; Time Factors; Urinary Bladder Neoplasms; Urinary Diversion; Quality of Life; Urinary Reservoirs Continent; Surgery; Oncology0302 clinical medicineCancer SurvivorsQuality of lifeSurveys and QuestionnairesOrthotopic neobladder80 and overSurveys and QuestionnaireStage (cooking)Aged 80 and overSettore MED/24 - UROLOGIABladder cancerUrinary Reservoirs ContinentGeneral MedicineMiddle AgedhumanitiesSurvival RateIleal conduitItalyOncology030220 oncology & carcinogenesisUrinary Bladder NeoplasmFemaleCancer SurvivorUrinary ReservoirsHumanQuality of lifeAdultmedicine.medical_specialtyTime FactorFollow-Up StudieCystectomy03 medical and health sciencesIleumInternal medicinemedicineHumansWomenPathologicalAgedCross-Sectional StudieBladder cancerbusiness.industryUrinary diversionCancermedicine.diseaseRadiation therapyRadical cystectomyContinentCross-Sectional StudiesBladder cancer; Ileal conduit; Orthotopic neobladder; Quality of life; Radical cystectomy; WomenUrinary Bladder NeoplasmsSurgerybusinessFollow-Up StudiesEuropean Journal of Surgical Oncology
researchProduct

Evaluation of B-cell secretion and peripheral insulin resistance during pregnancy and after delivery in gestational diabetes mellitus with obesity

1988

Nine pregnant women with gestational diabetes mellitus (GDM) were studied. Six normal pregnant women and six normal nonpregnant women were evaluated as control groups. All the women underwent oral glucose tolerance test and glucose clamp during the third trimester of pregnancy and two months after delivery. During OGTT, glucose, C-peptide and insulin plasma levels were determined. C-peptide and insulin values in the late phase of OGTT were higher during pregnancy than after delivery in both groups. In gestational diabetic women, the M-value in the second steady-state during glucose clamp was lower than in controls, both during pregnancy and after delivery. Nevertheless, in both groups the M…

Blood GlucoseAdultmedicine.medical_specialtyEndocrinology Diabetes and Metabolismmedicine.medical_treatmentPregnancy in DiabeticsObesity; Glucose Tolerance Test; Reference Values; Humans; Diabetes Mellitus; Islets of Langerhans; Insulin; Blood Glucose; Pregnancy; Puerperal Disorders; Adult; Insulin Resistance; Female; Pregnancy in DiabeticsIslets of LangerhansEndocrinologyInsulin resistanceReference ValuesPregnancyDiabetes mellitusInternal medicineInternal MedicinemedicineDiabetes MellitusHumansInsulinObesityPregnancyGlucose tolerance testSettore MED/12 - Gastroenterologiamedicine.diagnostic_testbusiness.industryInsulinGeneral MedicinePuerperal DisordersGlucose clamp techniqueGlucose Tolerance Testmedicine.diseaseGestational diabetesEndocrinologyGestationFemaleInsulin Resistancebusiness
researchProduct

Filling the gap between Guidelines and Real World in the cardiovascular approach to the diabetic patients: the need for a call to action

2020

Blood GlucoseAmerican diabetes associationmedicine.medical_specialtybusiness.industryLow density lipoprotein cholesterolCardiovascular outcome trials Cardiovascular prevention Cardiovascular risk Diabetes mellitus type 2 Glucose-lowering drugs Therapeutical targets Blood Glucose Humans Hypoglycemic Agents Cardiovascular Diseases Diabetes Mellitus Type 2 Sodium-Glucose Transporter 2 InhibitorsCall to actionProprotein Convertase Subtilisin/Kexin 9Diabetes Mellitus Type 2Cardiovascular preventionCardiovascular DiseasesHumansHypoglycemic AgentsMedicineCardiology and Cardiovascular MedicinebusinessIntensive care medicineSodium-Glucose Transporter 2 InhibitorsGlycated haemoglobin
researchProduct