Search results for " Nerve"

showing 10 items of 885 documents

Expression profiling of prospero in the Drosophila larval chemosensory organ: Between growth and outgrowth

2010

AbstractBackgroundThe antenno-maxilary complex (AMC) forms the chemosensory system of theDrosophilalarva and is involved in gustatory and olfactory perception. We have previously shown that a mutant allele of the homeodomain transcription factor Prospero (prosVoila1,V1), presents several developmental defects including abnormal growth and altered taste responses. In addition, many neural tracts connecting the AMC to the central nervous system (CNS) were affected. Our earlier reports on larval AMC did not argue in favour of a role ofprosin cell fate decision, but strongly suggested thatproscould be involved in the control of other aspect of neuronal development. In order to identify these fu…

Central Nervous SystemMESH : Transcription FactorsMESH: DrosophilaOF-FUNCTION SCREEN;MUSCA-DOMESTICA L;HOUSE-FLY LARVA;FINE-STRUCTURE;AXON GUIDANCE;TRANSCRIPTION FACTOR;PATTERN-FORMATION;GENETIC-ANALYSIS;NERVOUS-SYSTEMGenes InsectMESH: Genes InsectAXON GUIDANCEMUSCA-DOMESTICA L0302 clinical medicineMESH: Gene Expression Regulation DevelopmentalCluster AnalysisDrosophila ProteinsMESH: AnimalsTRANSCRIPTION FACTORMESH: Nerve Tissue ProteinsMESH : Nerve Tissue ProteinsOF-FUNCTION SCREENOligonucleotide Array Sequence AnalysisGenetics0303 health sciencesMESH : Central Nervous SystemMicrobiology and ParasitologyMESH : Genes InsectGene Expression Regulation DevelopmentalNuclear ProteinsMESH: Transcription FactorsNull alleleMicrobiologie et ParasitologieMESH : Oligonucleotide Array Sequence Analysis[ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]Larva[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]DrosophilaDrosophila ProteinResearch ArticleBiotechnologylcsh:QH426-470MESH: Drosophila Proteinslcsh:BiotechnologyNerve Tissue ProteinsBiotechnologiesBiology03 medical and health sciencesMESH: Gene Expression ProfilingGENETIC-ANALYSIS[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]lcsh:TP248.13-248.65GeneticsAnimalsMESH : Cluster AnalysisMESH: Central Nervous SystemAlleleMESH : DrosophilaAlleles030304 developmental biologyMESH : LarvaMicroarray analysis techniquesHOUSE-FLY LARVAGene Expression ProfilingMESH : Gene Expression ProfilingMESH: AllelesWild typeMESH : Nuclear ProteinsProsperobiology.organism_classificationMESH : Drosophila ProteinsMESH: Cluster AnalysisNERVOUS-SYSTEMGene expression profilinglcsh:GeneticsMESH: Oligonucleotide Array Sequence AnalysisHomeoboxMESH : AnimalsMESH : Gene Expression Regulation DevelopmentalMESH : AllelesMESH: Nuclear ProteinsMESH: Larva030217 neurology & neurosurgeryTranscription FactorsPATTERN-FORMATIONFINE-STRUCTURE
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Neuroblast pattern and identity in the Drosophila tail region and role of doublesex in the survival of sex-specific precursors.

2013

The central nervous system is composed of segmental units (neuromeres), the size and complexity of which evolved in correspondence to their functional requirements. In Drosophila, neuromeres develop from populations of neural stem cells (neuroblasts) that delaminate from the early embryonic neuroectoderm in a stereotyped spatial and temporal pattern. Pattern units closely resemble the ground state and are rather invariant in thoracic (T1-T3) and anterior abdominal (A1-A7) segments of the embryonic ventral nerve cord. Here, we provide a comprehensive neuroblast map of the terminal abdominal neuromeres A8-A10, which exhibit a progressively derived character. Compared with thoracic and anterio…

Central Nervous SystemMaleanimal structuresDoublesexSerial homologyApoptosisBiologyNeuroblastNeural Stem CellsAbdomenImage Processing Computer-AssistedAnimalsDrosophila ProteinsCell LineageMolecular BiologyBody PatterningSex CharacteristicsMicroscopy ConfocalNeuroectodermAnatomyNeuromereImmunohistochemistryNeural stem cellCell biologyDNA-Binding ProteinsVentral nerve cordDrosophilaFemaleGanglion mother cellDevelopmental BiologyDevelopment (Cambridge, England)
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Induction of identified mesodermal cells by CNS midline progenitors in Drosophila.

1997

ABSTRACT The Drosophila ventral midline cells generate a discrete set of CNS lineages, required for proper patterning of the ventral ectoderm. Here we provide the first evidence that the CNS midline cells also exert inductive effects on the mesoderm. Mesodermal progenitors adjacent to the midline progenitor cells give rise to ventral somatic mucles and a pair of unique cells that come to lie dorsomedially on top of the ventral nerve cord, the so-called DM cells. Cell ablation as well as cell transplantation experiments indicate that formation of the DM cells is induced by midline progenitors in the early embryo. These results are corroborated by genetic analyses. Mutant single minded embryo…

Central Nervous SystemMesodermanimal structuresSomatic cellCellEctodermNerve Tissue ProteinsBiologyMesodermCell MovementProto-Oncogene ProteinsmedicineMorphogenesisAnimalsDrosophila ProteinsProgenitor cellEye ProteinsMolecular BiologyEmbryonic InductionEpidermal Growth FactorCell growthGene Expression Regulation DevelopmentalMembrane ProteinsEmbryoAnatomyCell biologyDNA-Binding Proteinsmedicine.anatomical_structureDrosophila melanogasterVentral nerve cordembryonic structuresDevelopmental BiologySignal TransductionTranscription FactorsDevelopment (Cambridge, England)
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Morphological Characterization of the Entire Interneuron Population Reveals Principles of Neuromere Organization in the Ventral Nerve Cord ofDrosophi…

2011

Decisive contributions to our understanding of the mechanisms underlying the development of the nervous system have been made by studies performed at the level of single, identified cells in the fruit flyDrosophila. While all the motor neurons and glial cells in thoracic and abdominal segments of theDrosophilaembryo have been individually identified, few of the interneurons, which comprise the vast majority of cells in the CNS, have been characterized at this level. We have applied a single cell labeling technique to carry out a detailed morphological characterization of the entire population of interneurons in abdominal segments A1–A7. Based on the definition of a set of spatial parameters…

Central Nervous SystemNervous systemCell typeInterneuronCD8 AntigensGreen Fluorescent ProteinsLIM-Homeodomain ProteinsModels NeurologicalStatistics as TopicPopulationCell CountBiologyFunctional LateralityAnimals Genetically ModifiedInterneuronsNeural PathwaysmedicineAnimalsDrosophila ProteinsAmino Acidseducationeducation.field_of_studyGeneral NeurosciencefungiArticlesNeuromereAxonsmedicine.anatomical_structureVentral nerve cordDrosophilaAxon guidanceNeuroscienceDrosophila ProteinTranscription FactorsThe Journal of Neuroscience
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Hunchback is required for the specification of the early sublineage of neuroblast 7-3 in the Drosophila central nervous system.

2002

The Drosophila ventral nerve cord (VNC) derives from neuroblasts (NBs), which mostly divide in a stem cell mode and give rise to defined NB lineages characterized by specific sets of sequentially generated neurons and/or glia cells. To understand how different cell types are generated within a NB lineage, we have focused on the NB7-3 lineage as a model system. This NB gives rise to four individually identifiable neurons and we show that these cells are generated from three different ganglion mother cells (GMCs). The finding that the transcription factor Hunchback (Hb) is expressed in the early sublineage of NB7-3, which consists of the early NB and the first GMC (GMC7-3a) and its progeny (E…

Central Nervous SystemNeuronsCell typeLineage (genetic)biologyStem CellsGene ExpressionAnatomyCell fate determinationbiology.organism_classificationCell biologyDNA-Binding ProteinsDrosophila melanogasterNeuroblastMutagenesisVentral nerve cordAnimalsDrosophila ProteinsCell LineageStem cellDrosophila melanogasterMolecular BiologyDrosophila ProteinDevelopmental BiologyTranscription FactorsDevelopment (Cambridge, England)
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Programmed cell death in the embryonic central nervous system of Drosophila melanogaster.

2006

Although programmed cell death (PCD) plays a crucial role throughout Drosophila CNS development, its pattern and incidence remain largely uninvestigated. We provide here a detailed analysis of the occurrence of PCD in the embryonic ventral nerve cord (VNC). We traced the spatio-temporal pattern of PCD and compared the appearance of, and total cell numbers in,thoracic and abdominal neuromeres of wild-type and PCD-deficient H99mutant embryos. Furthermore, we have examined the clonal origin and fate of superfluous cells in H99 mutants by DiI labeling almost all neuroblasts, with special attention to segment-specific differences within the individually identified neuroblast lineages. Our data r…

Central Nervous SystemProgrammed cell deathanimal structuresEmbryo NonmammalianApoptosisCell CountBiologyNeuroblastInterneuronsmedicineAnimalsCell LineageMolecular BiologyBody PatterningNeuronsGene Expression Regulation DevelopmentalAnatomyNeuromerebiology.organism_classificationEmbryonic stem cellImmunohistochemistryCell biologyClone Cellsmedicine.anatomical_structureDrosophila melanogasternervous systemVentral nerve cordMutationNeuronDrosophila melanogasterGanglion mother cellDevelopmental BiologyDevelopment (Cambridge, England)
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The muscleblind gene participates in the organization of Z-bands and epidermal attachments of Drosophila muscles and is regulated by Dmef2.

1998

We report the embryonic phenotype of muscleblind (mbl), a recently described Drosophila gene involved in terminal differentiation of adult ommatidia. mbl is a nuclear protein expressed late in the embryo in pharyngeal, visceral, and somatic muscles, the ventral nerve cord, and the larval photoreceptor system. All three mbl alleles studied exhibit a lethal phenotype and die as stage 17 embryos or first instar larvae. These larvae are partially paralyzed, show a characteristically contracted abdomen, and lack striation of muscles. Our analysis of the somatic musculature shows that the pattern of muscles is established correctly, and they form morphologically normal synapses. Ultrastructural a…

Central Nervous SystemSomatic cellMuscle Fibers SkeletalNeuromuscular JunctionMuscle ProteinsGenes InsectBiologymuscle attachmentsmuscleblindMesodermTendonsEctodermAnimalsDrosophila ProteinsConnectinRNA MessengerNuclear proteinMuscle SkeletalMolecular BiologyZ-bandsCell NucleusEpidermis (botany)MyogenesisMEF2 Transcription FactorsDrosophila.Gene Expression Regulation DevelopmentalNuclear ProteinsEmbryoCell DifferentiationCell BiologyAnatomybacterial infections and mycosesEmbryonic stem cellPhenotypeCell biologyDNA-Binding ProteinsMyogenic Regulatory FactorsVentral nerve cordMutationInsect ProteinsDrosophilaPhotoreceptor Cells InvertebratemyogenesisDevelopmental BiologyTranscription FactorsDevelopmental biology
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The origin of postembryonic neuroblasts in the ventral nerve cord of Drosophila melanogaster.

1991

ABSTRACT Embryonic and postembryonic neuroblasts in the thoracic ventral nerve cord of Drosophila melanogaster have the same origin. We have traced the development of threefold-labelled single precursor cells from the early gastrula stage to late larval stages. The technique allows in the same individual monitoring of progeny cells at embryonic stages (in vivo) and differentially staining embryonic and postembryonic progeny within the resulting neural clone at late postembryonic stages. The analysis reveals that postembryonic cells always appear together with embryonic cells in one clone. Further-more, BrdU labelling suggests that the embryonic neuroblast itself rather than one of its proge…

Central Nervous Systemanimal structuresNeurogenesisClone (cell biology)BiologyNeuroblastNeuroblasts/dk/atira/pure/subjectarea/asjc/2700/2702AnimalsBrdUMolecular BiologyCell lineageNeuroblast proliferationStem CellsfungiEmbryogenesisCell BiologyAnatomyGastrulaEmbryonic stem cellCell biologyGastrulationDrosophila melanogasterBromodeoxyuridineVentral nerve cordDrosophilaAnatomy/dk/atira/pure/subjectarea/asjc/1300/1307Ganglion mother cellDevelopmental BiologyDevelopment (Cambridge, England)
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Differential effects of EGF receptor signalling on neuroblast lineages along the dorsoventral axis of the Drosophila CNS

1998

ABSTRACT The Drosophila ventral nerve cord derives from a stereotype population of about 30 neural stem cells, the neuroblasts, per hemineuromere. Previous experiments provided indications for inductive signals at ventral sites of the neuroectoderm that confer neuroblast identities. Using cell lineage analysis, molecular markers and cell transplantation, we show here that EGF receptor signalling plays an instructive role in CNS patterning and exerts differential effects on dorsoventral subpopulations of neuroblasts. The Drosophila EGF receptor (DER) is capable of cell autonomously specifiying medial and intermediate neuroblast cell fates. DER signalling appears to be most critical for prope…

Central Nervous Systemanimal structuresPopulationCell fate determinationBiologyNeuroblastEctodermAnimalseducationReceptorMolecular BiologyBody PatterningNeuronseducation.field_of_studyNeuroectodermStem CellsfungiAnatomyNeural stem cellCell biologyErbB Receptorsnervous systemVentral nerve cordMutationembryonic structuresDrosophilaGanglion mother cellBiomarkersSignal TransductionStem Cell TransplantationDevelopmental BiologyDevelopment
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Nitrergic modulation of gastrointestinal function during early endotoxemia.

2006

After bacterial infection, the host reacts by signalling to the central nervous system where a cascade of physiologic, neuroendocrine and behavioural processes is orchestrated, collectively termed the acute phase response. Endotoxemia following Gram-negative bacterial infection induces a wide array of effects, including fever, loss of appetite and changes in gastrointestinal function that attempt to eliminate the challenge and restore homeostasis. Systemic administration of low doses of endotoxin (5-40 microg/kg) to rats is associated with changes in gastrointestinal motor function, inhibition of gastric acid secretion and increase in the gastric mucosal resistance to damage. These changes …

Central Nervous Systemmedicine.medical_specialtyCentral nervous systemMyenteric PlexusNitric OxideNitric oxideGastric Acidchemistry.chemical_compoundInternal medicineNitrergic NeuronsDrug DiscoverymedicineAnimalsHumansPharmacologyGastrointestinal tractbiologyStomachVagus NerveEndotoxemiaRatsNitric oxide synthaseGastrointestinal Tractmedicine.anatomical_structureEndocrinologychemistryGastric MucosaRegional Blood Flowbiology.proteinGastric acidNitric Oxide SynthaseGastrointestinal functionGastrointestinal MotilityHomeostasisCurrent pharmaceutical design
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