Search results for " biosensor"

Rapid Assessment of Gray Mold (Botrytis cinerea) Infection in Grapes with a Biosensor System

2015

Abstract: Botrytis cinerea is the causative agent of gray mold disease, which causes considerable economic losses to winemakers. The extent of gray mold infection of winegrapes is commonly visually estimated, a method that is prone to assessor bias. Here, we used rapid and simple enzyme-based screening consisting of carbon-electrode, screen-printed amperometric biosensors to estimate gluconic acid and glycerol concentration in winegrapes infected with different degrees of B. cinerea. The lower limits of quantification of the screen-printed amperometric biosensors were 3 mg/L for gluconic acid (corresponding to an infection rate of less than 1%) and 35 mg/L for glycerol; the response times w…

A Review of Biosensors for Non-Invasive Diabetes Monitoring and Screening in Human Exhaled Breath

2019

Exhaled breath acetone has been identified as a diabetes biomarker for non-invasive diagnosis. Its detection using biosensors features has many advantages over the conventional means. This paper reviews the recent literature on the detection of exhaled breath acetone and acetone vapor of diabetic interest. The biosensors have been classified based on their transduction methods. The performance characteristics of the biosensors have been explored for comparison. The future trends are also highlighted.

Solution processed micro- and nano-arrays for multiplexed biosensing

2012

Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples.

2021

[EN] Candida auris has arisen as an important multidrug-resistant fungus because of several nosocomial outbreaks and elevated rates of mortality. Accurate and rapid diagnosis of C. auris is highly desired; nevertheless, current methods often present severe limitations and produce misidentification. Herein a sensitive, selective, and time-competitive biosensor based on oligonucleotide-gated nanomaterials for effective detection of C. auris is presented. In the proposed design, a nanoporous anodic alumina scaffold is filled with the fluorescent indicator rhodamine B and the pores blocked with different oligonucleotides capable of specifically recognize C. auris genomic DNA. Gate opening modul…

SMOOTHLY SHIFTING FLUORESCENT WINDOW: TUNABLE “OFF-ON-OFF”MICELLAR BIOSENSORS FOR pH

2009

Application of polydopamine functionalized zinc oxide for glucose biosensor design

2021

Zinc oxide (ZnO) nanostructures are widely used in optical sensors and biosensors. Functionalization of these nanostructures with polymers enables optical properties of ZnO to be tailored. Polydopamine (PDA) is a highly biocompatible polymer, which can be used as a versatile coating suitable for application in sensor and biosensor design. In this research, we have grown ZnO-based nanorods on the surface of ITO-modified glass-plated optically transparent electrodes (glass/ITO). Then the deposition of the PDA polymer layer on the surface of ZnO nanorods was performed from an aqueous PDA solution in such a way glass/ITO/ZnO-PDA structure was formed. The ZnO-PDA composite was characterized by S…

Amperometric Biosensor and Front-End Electronics for Remote Glucose Monitoring by Crosslinked PEDOT-Glucose Oxidase

2018

Focusing on the interplay between interface chemistry, electrochemistry, and integrated electronics, we show a novel low-cost and flexible biosensing platform for continuous glucose monitoring. The amperometric biosensing system features a planar three-electrode structure on a plastic substrate, and a wireless near-field communication-powered electronic system performing sensor analog front-end, A/D conversion, digital control, and display tasks. The working electrode is made of electropolymerized poly (3,4-ethylenedioxythiophene) film onto a polyethylene terephthalate/gold electrode followed by immobilization of cross-linked glucose oxidase by glutaraldehyde. The advantages offered by such…

Drug Screening by Inkjet Printing Microarrays

2010

Human Topoisomerase I interaction with DNA on flexible nylon substrate for malaria diagnosis

The study of interaction between human Topoisomerase I (hTopo I) and the appropriate sequences of DNA is shown, with the aim to realize a biosensor for malaria diagnosis. Topoisomerases alter the topological state of DNA by catalysing the breaking and rejoining of DNA strands1. The ligase activity is fundamental for our biosensing strategy, revealing the pathology. hTopo I was chosen as a model system for the Plasmodium analogous. The biosensor is a new flexible and easy to degrade biochip printed on nylon by dip-pen lithography (DPL). It is realized exploiting the high specificity of complementary ssDNA2 and hTopo I ligase activity. DPL operation needs ultra-low amounts of DNA (0.5 µL) to …

Highly sensitive olfactory biosensors for the detection of volatile organic compounds by surface plasmon resonance imaging

2018

International audience; Nowadays, monitoring of volatile organic compounds (VOCs) is very important in various domains. In this work, we aimed to develop sensitive olfactory biosensors using odorant binding proteins (OBPs) as sensing materials. Three rat OBP3 derivatives with customized binding properties were designed and immobilized on the same chip for the detection of VOCs in solution by surface plasmon resonance imaging (SPRi). We demonstrated that the proteins kept their binding properties after the immobilization under optimized conditions. The obtained olfactory biosensors exhibited very low limits of detection in both concentration (200pM of beta-ionone) and in molecular weight of …