Search results for " bodies"

July 2003: 62-year-old female with progressive muscular weakness

2004

The July 2003 Case of the Month (COM). A 62-year-old female patient experienced progressive muscular weakness over the last ten years, involving shoulder and pelvic girdle muscles, paraspinal and facial muscles. A biopsy was taken from the left deltoid muscle where hepatitis vaccination had taken place 4 weeks previously. The specimen revealed macrophagic myofasciitis due to the injection of aluminium-bound vaccines. The finding can be reproduced experimentally by injecting vaccines in rats. The pathomechanism is supposed to involve immune stimulation due to long term persistence of the adjuvant. Macrophagic myofasciitis has been suggested to occasionally cause myopathy but is supposed to b…

Crystalloid lysozyme inclusions in Paneth cells of vitamin A-deficient rats.

1990

The effect of vitamin A-deficiency on jejunal Paneth cells in rats was investigated. Crystalloid particles were observed in secretion granules of Paneth cells from 6 out of 8 rats with vitamin A-deficiency. The particles were similar to those found in Paneth cells under other experimental conditions. Using an immuno-electron-microscopic technique we demonstrated a clear lysozyme immunoreactivity of these particles. In 2 vitamin A-deficient rats tubular structures have been detected in addition to the crystalloid particles. Crystalloid particles or tubular structures were not detectable in a control group of 8 vitamin A-supplemented rats. The morphological alterations of Paneth cells may be …

Physiologic and biologic mechanisms induced in Yarrowia lipolytica in response to physico-chemical modifications of cells environment

2010

Hydrophobe compounds are known as carbon source which could be used by yeast like Yarrowia lipolytica for multi purposes. These compounds may cause disturbance in yeast but also reported as confer some resistance to cells towards environmental stress. Here, we study the role of methyl oleate as carbon source on resistance of Y. lipolytica in response to stress of an amphiphilic compound, -dodecalactone, and to heat shock. Results show that cells grown in oleate are more resistant to these stresses than cells grown in glucose. This work reveals the role of the lipids bodies in cells response to stress and that cells manifest in different ways in response to these stresses. An accumulation o…

Neurobiological Models of the Central Complex and the Mushroom Bodies

2013

This study reviews the actual knowledge on functions of the central complex (CX) and the mushroom bodies (MBs) in a genetic model insect, the fly Drosophila melanogaster. Ongoing research of UNIMAINZ and respective data are included. Reference is made to other insects, where respective functions are not yet studied in Drosophila. Neuroanatomical information is reported with regard to the general flow of information in these central brain neuropils. Particular projection systems and circuits are taken into account where this can be linked to functions. Models are developed.

The Role of Financial and Managerial Variables on the Policy towards Home-grown Players in English Soccer

2012

The training and development of young players both as athletes and individuals is one of the task soccer governing bodies assign to professional soccer team in order to fully exploit the social aspects of sport activities. Recently UEFA imposed new rules to improve the use of home-grown players. We analyze a panel data of English Premier and Championship league clubs for the seasons 2001-02 to 2005-06 in order to understand what clubs characteristics lead to the employment of young players in the team during official matches. Sport commercialization and the employment of star players have been considered harmful to this role. Our findings suggest that, counter-intuitively, economic and comm…

The dawning of the theory of equilibrium figures: a brief historical account from the 16th through the 20th century

2014

A very brief historical survey of the theory of equilibrium figures is outlined, from the early origins, dating back to the late 1500s, until up the latest 20th century developments. A seen toward physical applications, ranging from relativistic astrophysics through nuclear physics, is also provided.

Hsp60 from cancer cells can reach near and distant targets: A proposal for a multistage pathway

2011

Cancer cells have means to influence other cells in their vicinity and distant, and in this signal-delivering mechanisms the chaperonin Hsp60 plays a role, which is currently being recognized as potentially crucial for the growth and dissemination of at least certain types of tumors. In order to arrive at its destination, Hsp60, a typical resident of mitochondria in normal and tumor cells, leaves the organelle and reaches the blood. In the latter, Hsp60 can travel and arrive at targets situated far away from its origin. The details of the route followed by Hsp60 and their molecular mechanisms have not yet been fully elucidated. We investigated Hsp60 levels and secretion in normal and tumor …

2003

Jerboa (Jaculus orientalis) is a deep hibernating rodent native to subdesert highlands. During hibernation, a high level of ketone bodies i.e. acetoacetate (AcAc) and D-3-hydroxybutyrate (BOH) are produced in liver, which are used in brain as energetic fuel. These compounds are bioconverted by mitochondrial D-3-hydroxybutyrate dehydrogenase (BDH) E.C. 1.1.1.30. Here we report, the function and the expression of BDH in terms of catalytic activities, kinetic parameters, levels of protein and mRNA in both tissues i.e brain and liver, in relation to the hibernating process. We found that: 1/ In euthemic jerboa the specific activity in liver is 2.4- and 6.4- fold higher than in brain, respective…

Production of soluble eukaryotic recombinant proteins in E. coli is favoured in early log-phase cultures induced at low temperature

2013

Abstract Background Producing recombinant plant proteins expressed in Escherichia coli produce in high yields and in a soluble and functional form can be difficult. Under overexpression conditions, proteins frequently accumulate as insoluble aggregates (inclusion bodies) within the producing bacteria. We evaluated how the initial culture density, temperature and duration of the expression stage affect the production of some eukaryotic enzymes in E. coli. Findings A high yield of active soluble proteins was obtained by combining early-log phase cultures and low temperatures for protein induction. When IPTG was added at OD600 = 0.1 and cultures were maintained at 4°C for 48-72 h, the soluble …

Glucose, Lactate, and Ketone Body Utilization by Human Mammary Carcinomas in Vivo

1985

Uncontrolled growth, one of the fundamental properties of malignant tumors, requires a great supply of energy. This energy can be derived from the use of a variety of substrates. Besides glucose oxidation and glucose breakdown to lactic acid, the turnover of endogeneous substrates such as amino acids, free fatty acids and ketone bodies is well documented in vitro. However, under in vivo conditions, only glucose utilization has been investigated in detail, using tumor isotransplants in rodents. For human tumors, only scarce data is available, derived mainly from clinical observations rather than from systematic studies.