Search results for " samples"

showing 10 items of 125 documents

HPTLC Phenolic Profiles as Useful Tools for the Authentication of Honey

2018

The present study reveals the utility of high-performance thin-layer chromatographic (HPTLC) fingerprinting of phenolic constituents for the authentication of monofloral honeys. The obtained data enables a more complete assessment of honey quality and the identification of emerging threats to honey quality. The developed procedure facilitates differentiation of varietal honeys and detection of honey adulterations. We used an HPTLC fingerprint analysis to determine the characteristic patterns of different honey types (willow, buckwheat, heather, pine honeydew, and manuka honey). The HPTLC chromatograms were used to determine the differences in the botanical origin of the honey samples on the…

HPTLC fingerprintHplc analysisHoneydewChromatographyHptlc fingerprintChemistry010401 analytical chemistryhoneyphenolic compounds04 agricultural and veterinary sciences040401 food science01 natural sciencesApplied Microbiology and BiotechnologyHoney samplesManuka Honey0104 chemical sciencesAnalytical Chemistry0404 agricultural biotechnologyhigh-performance thin-layer chromatographySafety Risk Reliability and QualitySafety ResearchFood ScienceFood Analytical Methods
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Trends in the prevalence of asthma and allergic rhinitis in Italy between 1991 and 2010

2012

The prevalence of asthma increased worldwide until the 1990s, but since then there has been no clear temporal pattern. The present study aimed to assess time trends in the prevalence of current asthma, asthma-like symptoms and allergic rhinitis in Italian adults from 1990 to 2010. The same screening questionnaire was administered by mail or phone to random samples of the general population (age 20-44 yrs) in Italy, in the frame of three multicentre studies: the European Community Respiratory Health Survey (ECRHS) (1991-1993; n=6,031); the Italian Study on Asthma in Young Adults (ISAYA) (1998-2000; n=18,873); and the Gene Environment Interactions in Respiratory Diseases (GEIRD) study (2007-2…

MalePediatricsCross-sectional study95% CI 1.19-1.59) from 1998-2000 to 2007-2010but since then there has been no clear temporal pattern. The present study aimed to assess time trends in the prevalence of current asthmaAbstract The prevalence of asthma increased worldwide until the 1990stemporal trendsSurveys and QuestionnairesEpidemiologySurveys and QuestionnaireMedicineasthma-like symptoms and allergic rhinitis in Italian adults from 1990 to 2010. The same screening questionnaire was administered by mail or phone to random samples of the general population (age 20-44 yrs) in ItalyYoung adultrespectively. The prevalence of current asthma was stable during the 1990s and increased (relative risk 1.38from 10.1% to 13.9% and from 16.8% to 25.8%education.field_of_studyAllergic rhinitis; Asthma; Epidemiology; Prevalence; Temporal trends; Wheezing;medicine.diagnostic_testSmokingthe median prevalence of current asthmaallergic rhinitis asthma epidemiology prevalence temporal trends wheezingItalyAbstract The prevalence of asthma increased worldwide until the 1990s but since then there has been no clear temporal pattern. The present study aimed to assess time trends in the prevalence of current asthma asthma-like symptoms and allergic rhinitis in Italian adults from 1990 to 2010. The same screening questionnaire was administered by mail or phone to random samples of the general population (age 20-44 yrs) in Italy in the frame of three multicentre studies: the European Community Respiratory Health Survey (ECRHS) (1991-1993; n=6031); the Italian Study on Asthma in Young Adults (ISAYA) (1998-2000; n=18873); and the Gene Environment Interactions in Respiratory Diseases (GEIRD) study (2007-2010; n=10494). Time trends in prevalence were estimated using Poisson regression models in the centres that repeated the survey at different points in time. From 1991 to 2010 the median prevalence of current asthma wheezing and allergic rhinitis increased from 4.1% to 6.6% from 10.1% to 13.9% and from 16.8% to 25.8% respectively. The prevalence of current asthma was stable during the 1990s and increased (relative risk 1.38 95% CI 1.19-1.59) from 1998-2000 to 2007-2010 mainly in subjects who did not report allergic rhinitis. The prevalence of allergic rhinitis has increased continuously since 1991. The asthma epidemic is not over in Italy. During the past 20 yrs asthma prevalence has increased by 38% in parallel with a similar increase in asthma-like symptoms and allergic rhinitis.mainly in subjects who did not report allergic rhinitis. The prevalence of allergic rhinitis has increased continuously since 1991. The asthma epidemic is not over in Italy. During the past 20 yrssymbolsasthma prevalence has increased by 38%FemaleepidemiologyHumanPulmonary and Respiratory MedicineAdult494). Time trends in prevalence were estimated using Poisson regression models in the centres that repeated the survey at different points in time. From 1991 to 2010in parallel with a similar increase in asthma-like symptoms and allergic rhinitismedicine.medical_specialtyRhinitis Allergic PerennialPopulationprevalencethe Italian Study on Asthma in Young Adults (ISAYA) (1998-2000Settore MED/10 - Malattie Dell'Apparato Respiratorio031)and the Gene Environment Interactions in Respiratory Diseases (GEIRD) study (2007-2010Settore MED/01 - Statistica Medicasymbols.namesakeYoung AdultAllergic rhinitiHumansRespiratory soundsPoisson regressioneducationAsthmaRespiratory SoundsCross-Sectional Studieallergic rhinitisbusiness.industrywheezingwheezing and allergic rhinitis increased from 4.1% to 6.6%Rhinitis Allergic Seasonalasthmain the frame of three multicentre studies: the European Community Respiratory Health Survey (ECRHS) (1991-1993medicine.disease873)Cross-Sectional Studiesn=10Relative riskTemporal trendRespiratory Soundn=6n=18business
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Determination of amobarbital and secobarbital in plasma samples using micellar liquid chromatography

2000

A new liquid chromatographic procedure for the determination of amobarbital and secobarbital in plasma samples is proposed. The method uses a Spherisorb octadecylsilane ODS-2 C18 analytical column, a guard column of similar characteristics and 0.04 M CTAB solution buffered at pH 7.5 containing 3% 1-propanol as micellar mobile phase. The UV detection was carried out at 250 nm. Butabarbital was used as internal standard. Plasma samples preparation only required adequate dilution with the mobile phase before injection into the chromatographic system. The limits of detection were 0.2 and 0.4 mg/L for amobarbital and secobarbital, respectively. The proposed method allows the determination of amo…

PharmacologyDetection limitChromatographyPlasma samplesChemistryButabarbitalAmobarbitalClinical BiochemistryAnalytical chemistryGeneral MedicineSecobarbitalBiochemistryAnalytical ChemistryDilutionMicellar liquid chromatographyDrug DiscoverymedicineUv detectionMolecular Biologymedicine.drugBiomedical Chromatography
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Characterization of the bacterial community isolated from a High Middle Age soil sample dated since 980 AD

2016

Paleomicrobiology ancient samples bacterial community antibiotics secreted proteasesSettore BIO/19 - Microbiologia Generale
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A new method for fluoride determination by using fluorophores and dyes anchored onto MCM-41

2002

A new colourimetric and fluorimetric method for fluoride determination in aqueous samples based on the specific reaction between fluoride and silica has been developed and applied on real samples. Descalzo Lopez, Ana Belen, adeslo@alumni.uv.es ; El Haskouri, Jamal, Jamal.Haskouri@uv.es ; Beltran Porter, Daniel, Daniel.Beltran@uv.es ; Amoros del Toro, Pedro Jose, Pedro.Amoros@uv.es

SamplesReactionUNESCO::QUÍMICAUNESCO::QUÍMICA::Química analítica:QUÍMICA::Química analítica [UNESCO]FluorophoresFluoride detrmination ; Fluorophores ; Reaction ; Samples:QUÍMICA [UNESCO]Fluoride detrmination
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From the Beehives: Identification and Comparison of Physicochemical Properties of Algerian Honey

2021

In this study, the authors aimed at characterizing 11 Algerian kinds of honey taken from various geographical locations (beehives located at Djelfa (Medjbara and Dzaira), Laghouat, Aflou, Medea, Tiaret, Sidi bel-Abbes, Tiaret, Ain-Safra, Mostaganem, El Bayadh, and Ghardïa). The authors investigated the physicochemical parameters of these honey samples, including density, water content, electrical conductivity, ash content, pH, hydroxymethylfurfural (HMF) content, free acidity, and color. The physicochemical parameters obtained were found to be within acceptable ranges according to the international standards (Codex Alimentarius) for 9 out of 11 analyzed samples: density 1.38–1.50 g/cm3 (the…

SciencefoodQphysicochemical parametershydroxymethylfurfuralhoneyManagement Monitoring Policy and LawHoney sampleschemistry.chemical_compoundchemistryqualityAlgeriaFood scienceWater contentHydroxymethylfurfuralphysicochemical analysisNature and Landscape ConservationResources
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A pilot study on non-invasive treatment of migraine: The self-myofascial release

2021

The aims of this paper was to determine the effect of self-myofascial release (SMFR) on postural stability and to analyze if it can influence migraine condition. Twenty-five subjects (age 49.7±12.5) affected by migraine were enrolled. Assessments included a stabilometric analysis in order to evaluate balance and plantar support, with eyes open (OE) and closed (CE); cervical ROM measurement; evaluation of upper limb strength through handgrip. All the analysis were carried out before and after the administration of a single SMFR protocol, using medium density small balls laid in the three most painful trigger points in migraine patients: trapezius, sternocleidomastoids and suboccipital muscle…

medicine.medical_specialtymyofascial release techniques fascia balance cervical ROMlcsh:MedicineArticlelcsh:QM1-69503 medical and health sciences0302 clinical medicinePhysical medicine and rehabilitationPaired samplesMedicineOrthopedics and Sports MedicineMolecular BiologyMigrainefasciaBalance (ability)030222 orthopedicsbusiness.industrylcsh:RNon invasivebalancelcsh:Human anatomyCell BiologyMedium densitymedicine.diseasecervical ROMMyofascial releasemyofascial release techniquesmedicine.anatomical_structureMigrainePostural stabilityUpper limbNeurology (clinical)business030217 neurology & neurosurgeryEuropean Journal of Translational Myology
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P27-S Sex differences and cortical excitability in healthy adolescents

2019

Background Sex is known to influence brain development. However, sex differences in cortical excitability and their relationship to motor functions is unclear. Aim We examined the sex related differences in the degree of handedness, left and right motor cortex excitability and manual dexterity. Methods 28 healthy adolescents (age 16–19 years,19 girls) were studied. The degree of right-handedness was determined by the Waterloo Handedness Questionnaire. Motor threshold (MT) of the abductor pollicis brevis was measured on both hemispheres. Box and block test (BBT) was used for manual dexterity. Independent samples t-test was used in statistical analysis. Results There were no differences betwe…

Motor thresholdmedicine.medical_specialtyBrain developmentbusiness.industrySex relatedAudiologySensory SystemsLateralization of brain functionmedicine.anatomical_structureNeurologyPhysiology (medical)Independent samplesmedicineStatistical analysisNeurology (clinical)Right hemispherebusinessMotor cortexClinical Neurophysiology
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R-Score: A New Parameter to Assess the Quality of Variants’ Calls Assessed by NGS Using Liquid Biopsies

2021

Next-generation sequencing (NGS) has enabled a deeper knowledge of the molecular landscape in non-small cell lung cancer (NSCLC), identifying a growing number of targetable molecular alterations in key genes. However, NGS profiling of liquid biopsies risk for false positive and false negative calls and parameters assessing the quality of NGS calls remains lacking. In this study, we have evaluated the positive percent agreement (PPA) between NGS and digital PCR calls when assessing EGFR mutation status using 85 plasma samples from 82 EGFR-positive NSCLC patients. According to our data, variant allele fraction (VAF) was significantly lower in discordant calls and the median of the absolute va…

Oncologymedicine.medical_specialtyKey genesQH301-705.5BiologyGeneral Biochemistry Genetics and Molecular BiologyArticle03 medical and health sciences0302 clinical medicineInternal medicinemedicineDigital polymerase chain reactionBiology (General)Liquid biopsy030304 developmental biology0303 health sciencesGeneral Immunology and MicrobiologyPlasma samplesliquid biopsyvariant callingVariant allelectDNArespiratory systemfiltering3. Good healthEgfr mutation030220 oncology & carcinogenesisNGSVAFNon small cellLinear correlationGeneral Agricultural and Biological SciencesBiology
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Differences between Human Plasma and Serum Metabolite Profiles

2011

BackgroundHuman plasma and serum are widely used matrices in clinical and biological studies. However, different collecting procedures and the coagulation cascade influence concentrations of both proteins and metabolites in these matrices. The effects on metabolite concentration profiles have not been fully characterized.Methodology/principal findingsWe analyzed the concentrations of 163 metabolites in plasma and serum samples collected simultaneously from 377 fasting individuals. To ensure data quality, 41 metabolites with low measurement stability were excluded from further analysis. In addition, plasma and corresponding serum samples from 83 individuals were re-measured in the same plate…

MaleSerumClinical Research DesignEpidemiologyScienceMetaboliteProtein metabolismType 2 diabetesPharmacologyBiologyBiochemistryPlasmachemistry.chemical_compoundDiagnostic MedicineBlood plasmaPathologymedicineMetabolomeHumansClinical EpidemiologyBiologyAgedAged 80 and overClinical ChemistryReproducibilityMultidisciplinaryChromatographyQChromatography; Metabolomics; Collection; Samples; Issues; AcidRReproducibility of ResultsMiddle Agedmedicine.diseaseClinical Laboratory SciencesBiomarker EpidemiologychemistrySmall MoleculesBlood ChemistryMetabolomeMedicineBiomarker (medicine)FemaleBiomarkersDrug metabolismResearch ArticleGeneral PathologyPLoS ONE
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