Search results for "53"
showing 10 items of 2908 documents
Apoptosis induced by MNNG in human TK6 lymphoblastoid cells is p53 and Fas/CD95/Apo-1 related.
2003
Agents inducing O(6)-methylguanine (O(6)MeG) in DNA, such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), are not only highly mutagenic and carcinogenic but also cytotoxic because of the induction of apoptosis. In CHO fibroblasts, apoptosis triggered by O(6)MeG requires cell proliferation and MutSalpha-dependent mismatch repair and is related to the induction of DNA double-strand breaks (DSBs). Furthermore, it is mediated by Bcl-2 degradation and does not require p53 for which the cells were mutated [Cancer Res. 60 (2000) 5815]. Here we studied cytotoxicity and apoptosis induced by MNNG in a pair of human lymphoblastoid cells expressing wild-type p53 (TK6) and mutant p53 (WTK1) and show tha…
O6-methylguanine DNA methyltransferase and p53 status predict temozolomide sensitivity in human malignant glioma cells
2006
Temozolomide (TMZ) is a methylating agent which prolongs survival when administered during and after radiotherapy in the first-line treatment of glioblastoma and which also has significant activity in recurrent disease. O6-methylguanine DNA methyltransferase (MGMT) is a DNA repair enzyme attributed a role in cancer cell resistance to O6-alkylating agent-based chemotherapy. Using a panel of 12 human glioma cell lines, we here defined the sensitivity to TMZ in acute cytotoxicity and clonogenic survival assays in relation to MGMT, mismatch repair and p53 status and its modulation by dexamethasone, irradiation and BCL-X(L). We found that the levels of MGMT expression were a major predictor of T…
Establishment and characterization of a nontumorigenic cell line derived from a human hepatocellular adenoma expressing hepatocyte-specific markers.
1997
In the present study the establishment and characterization of a nontumorigenic liver epithelial cell line (HACL-1) derived from a human hepatocellular adenoma is described. The HACL-1 cells have a finite life span (i.e., they proliferate for a period of 2 months and then senesce), show cell-cell contact inhibition, do not grow in soft agar, are not tumorigenic when injected in nude mice, and possess a normal diploid karyotype. The cultured cells resemble hepatocytes, but exhibit some features of dedifferentiation. At the ultrastructural level the cells are endowed with round or oval nuclei, abundant cytoplasmic organelles, and varying amounts of glycogen. The rough endoplasmic reticulum is…
Force-induced diffusion in microrheology
2012
We investigate the force-induced diffusive motion of a tracer particle inside a glass-forming suspension when a strong external force is applied to the probe (active nonlinear microrheology). A schematic model of mode-coupling theory introduced recently is extended to describe the transient dynamics of the probe particle, and used to analyze recent molecular-dynamics simulation data. The model describes non-trivial transient displacements of the probe before a steady-state velocity is reached. The external force also induces diffusive motion in the direction perpendicular to its axis. We address the relation between the transverse diffusion coefficient D(perpendicular) and the force-depende…
Concurrent magneto-optical imaging and magneto-transport readout of electrical switching of insulating antiferromagnetic thin films
2020
We demonstrate stable and reversible current induced switching of large-area ($> 100\;��m^2$) antiferromagnetic domains in NiO/Pt by performing concurrent transport and magneto-optical imaging measurements in an adapted Kerr microscope. By correlating the magnetic images of the antiferromagnetic domain changes and magneto-transport signal response in these current-induced switching experiments, we disentangle magnetic and non-magnetic contributions to the transport signal. Our table-top approach establishes a robust procedure to subtract the non-magnetic contributions in the transport signal and extract the spin-Hall magnetoresistance response associated with the switching of the antifer…
Toward fast malaria detection by secondary speckle sensing microscopy
2012
Diagnosis of malaria must be rapid, accurate, simple to use, portable and low cost, as suggested by the World Health Organization (WHO). Despite recent efforts, the gold standard remains the light microscopy of a stained blood film. This method can detect low parasitemia and identify different species of Plasmodium. However, it is time consuming, it requires well trained microscopist and good instrumentation to minimize misinterpretation, thus the costs are considerable. Moreover, the equipment cannot be easily transported and installed. In this paper we propose a new technique named "secondary speckle sensing microscopy" ((SM)-M-3) based upon extraction of correlation based statistics of s…
Movilidad humana: una revisión teórica aplicable de los flujos migratorios en España
2020
El texto tiene el objetivo de realizar una revisión teórica aplicable entorno a las migraciones en el contexto particular español. Se ha realizado una revisión bibliográfica aplicable disponible en torno al tema, con el fin de ofrecer una recopilación y selección de la información disponible a este respecto partiendo de la dicotomía que haría en su momento López de Lera (1995), los que “vienen a descansar y los que vienen a trabajar”. Es importante porque el desarrollo teórico de sendas perspectivas de la migración es divergente. Este es el aporte del texto, realizar un recorrido por los dos caminos migratorios y delimitar las principales corrientes.
1,4 - dihidropiridīna atvasinājumu (AV-153-sāļu) mijiedarbības izpēte pret c-Myc antiparalēlo kvadrupleksu, izmantojot UV/redzamo spektroskopiju
2020
Pedējos gados ievērojami pastiprinājusies uzmanība pret DNS guanīna kvadrupleksiem un to lomu onkoģenēzē. c-Myc proto-onkogēna pastiprināta ekspresija ir saistīta ar dažādiem cilvēka audzējiem, ka arī ar slikto prognozi. Līdz ar to DNS guanīna kvadrupleksu veidošana un stabilizācija C-myc promoter reģionos ir kritiska c-Myc trankripcijas inhibēšanai, tāpēc pēdējos gados aktīvi tiek meklēti savienojumi, kuri var stabilizēt G-kvadrupleksu struktūras un inhibēt c-Myc gēna ekspresiju. Šī pētījuma darba mērķis bija izpētīt 1,4-dihidropiridīna atvasinājumu (AV-153 sāļu un to analoga AV-154-Na sāls) spēju mijiedarboties ar antiparalēlo c-myc kvadrupleksu, kā arī spēju mijiedarboties ar vienpavadie…
Immunohistochemical/histochemical double staining method in the study of the columnar metaplasia of the oesophagus
2014
Intestinal metaplasia in Barrett’s oesophagus (BO) represents an important risk factor for oesophageal adenocarcinoma. Instead, few and controversial data are reported about the progression risk of columnar-lined oesophagus without intestinal metaplasia (CLO), posing an issue about its clinical management. The aim was to evaluate if some immunophenotypic changes were present in CLO independently of the presence of the goblet cells. We studied a series of oesophageal biopsies from patients with endoscopic finding of columnar metaplasia, by performing some immunohistochemical stainings (CK7, p53, AuroraA) combined with histochemistry (Alcian-blue and Alcian/PAS), with the aim of simultaneousl…
Gracia y gobierno en la administración valenciana del seiscientos. Hacia nuevos consensos
2015
Desde 1645 dos miembros de la nobleza local entraron a formar parte de la sala criminal de la Audiencia valenciana. Como consejeros de capa y espada asumieron competencias en materias de gracia y gobierno. Con esta medida se introdujo una moderación del arbitrio virreinal en tales ejercicios. Pero, sobre todo, la corona convirtió a la pequeña y mediana nobleza del reino en nueva colaboradora de su política de control estamental. From 1645 two members of the local nobility came to form part of the Criminal Chamber of the Valencian audience. As Ministers of «capa y espada» took powers in matters of Grace and Government. This measure was introduced a moderation of the Viceregal discretion in s…