Search results for "AUV"

3-Oxo-rhazinilam:  A New Indole Alkaloid from Rauvolfia serpentina × Rhazya stricta Hybrid Plant Cell Cultures

2000

A new monoterpenoid indole alkaloid, 3-oxo-rhazinilam (1), was isolated from intergeneric somatic hybrid cell cultures of Rauvolfia serpentina and Rhazya stricta, and the structure was determined by detailed 1D and 2D NMR analysis. It was also proved that 3-oxo-rhazinilam (1) is a natural constituent of the hybrid cells.

Isolation and Structure Elucidation of a New Indole Alkaloid from Rauvolfia serpentina Hairy Root Culture: The First Naturally Occurring Alkaloid of …

2002

A new monoterpenoid indole alkaloid, 10-hydroxy- N(alpha)-demethyl-19,20-dehydroraumacline ( 1), was isolated as a mixture of E- and Z-isomers from hairy root culture of Rauvolfia serpentina Benth. ex Kurz (Apocynaceae) and the structure was determined by 1D and 2D NMR analyses. The new indole alkaloid represents the first naturally occurring alkaloid of the raumacline group and its putative biosynthetical pathway is discussed.

In vivo monitoring of alkaloid metabolism in hybrid plant cell cultures by 2D cryo-NMR without labelling

2003

Non-invasive measurements of alkaloid metabolism in plant cell suspension cultures of a somatic hybrid from Rauvolfia serpentina Benth. ex Kurz and Rhazya stricta Decaisne were carried out. When cell samples were taken sequentially from a stock feeding experiment, measuring times for in vivo NMR of 40 min were sufficient for following conversions of alkaloids at the natural abundance of 13C. Degradation of ajmaline added to the cells at 1.6 mM concentration to raumacline could be monitored after 96 h on a standard 800 MHz NMR instrument (Avance 800). Feeding vinorine an intermediate of ajmaline biosynthesis at 1.8 mM showed with a 500 MHz CryoProbe that the alkaloid enters two metabolic rou…

Co-occurrence and risk assessment of mycotoxins in food and diet from Mediterranean area.

2012

The contents of 14 mycotoxins were studied in samples of different cereals and cereal products from four countries of the Mediterranean region. Two hundred and sixty-five samples from Spain, Italy, Morocco and Tunisia were analysed. Samples were extracted with matrix solid-phase dispersion (MSPD) and determined by liquid chromatography-tandem mass spectrometry with a triple quadrupole mass analyser. The percentage of total samples contaminated was 53%. The frequency of contaminated samples from Spain, Italy, Tunisia and Morocco was 33%, 52%, 96% and 50%, respectively. Nivalenol and beauvericin were the most predominant mycotoxins. This is the first international report to study the presence…

Beauvericin exposure induces cell cycle arrest, cell death and mitochondrial membrane potential disruption in CHO-K1 Cells

2015

DETERMINATION OF THE OPTIMAL MOMENT OF HARVESTING FOR CABERNET SAUVIGNON AND MERLOT GRAPES IN DRĂGĂŞANI VINEYARD

2011

This paper presents an analysis of the main characteristics of black grapes during ripening and determining the optimum moment to harvest the grapes in the vineyard Drăgăşani. Monitoring process of maturation of the grapes has been done on the varieties of grapes for red wines Cabernet Sauvignon and Merlot from Drăgăşani vineyard.

Toxicological Study in vitro, in vivo and Transcriptomic of Mycotoxins. Estudio Toxicológico in vitro, in vivo y Transcriptómico de Micotoxinas

2018

Las micotoxinas, producidas por el metabolismo secundario de hongos filamentosos, se consideran contaminantes comunes de alimentos y piensos y constituyen una preocupación en el ámbito de la seguridad alimentaria. Se han estudiado los efectos tóxicos y mecanismos de acción de las micotoxinas emergentes Beauvericina (BEA) y Eniatinas (ENs) mediante ensayos in vitro en células Jurkat, in vivo en rata Wistar y transcriptómicos por secuenciación del ARN; así como la presencia de multi-micotoxinas en alimentos, piensos y muestras biológicas. Las revisiones realizadas ponen de manifiesto que (i) la investigación in vivo de fusarotoxinas se basa principalmente en estudios de toxicidad subaguda y s…

Gender in translating lesbianism in The Second Sex

2011

Drawing on three Chinese translations of the lesbian chapter in Simone de Beauvoir’s The Second Sex, this paper examines at the micro level how the discussions on lesbianism in the work are translated and what role gender identity plays in the process of translation. Lesbianism has played an important part in feminism. However, homosexuality is still regarded as unacceptable to most Chinese people. Thus, an examination of translations of lesbianism in The Second Sex might be especially revealing in terms of representation of Western feminism. The comparison reveals that there are obvious differences between the translations by the female translators and the male translators. The differences…

Structures of Alkaloid Biosynthetic Glucosidases Decode Substrate Specificity

2011

Two similar enzymes with different biosynthetic function in one species have evolved to catalyze two distinct reactions. X-ray structures of both enzymes help reveal their most important differences. The Rauvolfia alkaloid biosynthetic network harbors two O-glucosidases: raucaffricine glucosidase (RG), which hydrolyses raucaffricine to an intermediate downstream in the ajmaline pathway, and strictosidine glucosidase (SG), which operates upstream. RG converts strictosidine, the substrate of SG, but SG does not accept raucaffricine. Now elucidation of crystal structures of RG, inactive RG-E186Q mutant, and its complexes with ligands dihydro-raucaffricine and secologanin reveals that it is the…

Ligand structures of synthetic deoxa-pyranosylamines with raucaffricine and strictosidine glucosidases provide structural insights into their binding…

2014

Insight into the structure and inhibition mechanism of O-β-d-glucosidases by deoxa-pyranosylamine type inhibitors is provided by X-ray analysis of complexes between raucaffricine and strictosidine glucosidases and N-(cyclohexylmethyl)-, N-(cyclohexyl)- and N-(bromobenzyl)-β-d-gluco-1,5-deoxa-pyranosylamine. All inhibitors anchored exclusively in the catalytic active site by competition with appropriate enzyme substrates. Thus facilitated prospective elucidation of the binding networks with residues located at <3.9 A distance will enable the development of potent inhibitors suitable for the production of valuable alkaloid glucosides, raucaffricine and strictosidine, by means of synthesis in …