Search results for "Anion"

Determination of total ribonucleotide pool in plant materials by high-pH anion-exchange high-performance liquid chromatography following extraction w…

2005

A new, improved method that only requires a potassium hydroxide extraction procedure is presented for the analysis of a full nucleotide pool in plant materials. Quantification was performed by high-pH anion-exchange chromatography (HPAEC) with UV detection after a potassium hydroxide extraction, and allowed the quantification of 13 linear ribonucleotides in a single run. The method has been validated by comparison of six extraction methods and also by measurement of the intracellular nucleotide levels of three plant species (cell cultures and leaves). The evolution of the nucleotide pool of Nicotiana tabacum cell culture during growth has also been measured, and showed an increase in the po…

Uric Acid Metabolism in Pre-hypertension and the Metabolic Syndrome

2014

In humans uric acid (UA) is the end product of degradation of purines. The handling of UA by the renal system is a complex process which is not fully understood. To date, several urate transporters in the renal proximal tubule have been identified. Among them, urate transporter 1 (URAT1) and a glucose transporter 9 (GLUT9) are considered of greater importance, as potential targets for treatment of hyperuricemia and the potential associated cardio-metabolic risk. Therefore, the recognition of the metabolic pathway of UA and elucidation of occurrence of hyperuricemia may provide important insights about the relationship between UA, pre-hypertension (preHT) and the metabolic syndrome (MetS). W…

Characterization of the Viable but Nonculturable (VBNC) State in Saccharomyces cerevisiae

2013

The Viable But Non Culturable (VBNC) state has been thoroughly studied in bacteria. In contrast, it has received much less attention in other microorganisms. However, it has been suggested that various yeast species occurring in wine may enter in VBNC following sulfite stress.In order to provide conclusive evidences for the existence of a VBNC state in yeast, the ability of Saccharomyces cerevisiae to enter into a VBNC state by applying sulfite stress was investigated. Viable populations were monitored by flow cytometry while culturable populations were followed by plating on culture medium. Twenty-four hours after the application of the stress, the comparison between the culturable populat…

Acid excreting mutants of yeast Saccharomyces cerevisiae.

2004

Saccharomyces cerevisiae mutants acidifying glucose medium containing bromocresol purple were shown to excrete protons when placed in unbuffered water in the absence of any external carbon source. The mutants belong to 16 different complementation groups. Most of them do not grow on glycerol and the excreted protons are associated to particular sets of organic anions such as citrate, aconitate, succinate, fumarate or malate. These novel types of respiratory mutations seem to be located in genes operating in the Krebs or glyoxylate cycle.

Selective and Sensitive Chromogenic Detection of Cyanide and HCN in Solution and in Gas Phase

2013

Two triphenylmethane based chemodosimeters for selective and chromogenic sensing of cyanide anions in aqueous environments and of hydrogen cyanide in gas phase were prepared and studied.

A preliminary study on anion binding ability of geminal diimidazolium salts

Liver and Statins: A Critical Appraisal of the Evidence.

2019

Adverse drug reactions (ADRs) represent an important cause of morbidity and mortality worldwide. Statins are a class of drugs whose main adverse effects are drug-induced liver injury (DILI) and myopathy. Some of these may be predictable, due to their pharmacokinetic and pharmacodynamic properties, while others, unfortunately, are idiosyncratic. Genetic factors may also influence patient susceptibility to DILI and myopathy in the case of statins. This review will first discuss the role of statins in cardiovascular disease treatment and prevention and the underlying mechanisms of action. Furthermore, to explore the susceptibility of statin-induced adverse events such as myopathy and hepatoto…

Design of carborane molecular architectures with electronic structure computations: From endohedral and polyradical systems to multidimensional netwo…

2009

11 pags, 6 figs. -- 19th International Conference on Physical Organic Chemistry (ICPOC-19) 13–18 July 2008, Santiago de Compostela, Spain

Evaluation of advanced silica packings for the separation of biopolymers by high-performance liquid chromatography

1989

Abstract The linear solvent strength model of Snyder was applied to describe fast protein separations on 2.1-μm non-porous, silica-based strong anion exchangers. It was demonstrated on short columns packed with these anion exchangers that (i) a substantially higher resolution of proteins and nucleotides was obtained at gradient times of less than 5 min than on porous anion exchangers; (ii) the low external surface area of the non-porous anion exchanger is not a critical parameter in analytical separations and (iii) μg-amounts of enzymes of high purity and full biological activity were isolated.

Über die elementarprozesse bei der anionischen polymerisation des styrols in tetrahydropyran (THP)

1972

Kinetische und konduktometrische Messungen am System Polystyrl-Natrium in THP werden mit dem Ziel durchgefuhrt, alle in dem fruher vorgeschlagenen dreiteiligen Reaktionsschema vorkommenden thermodynamischen und kinetischen Parameter zu bestimmen und mit den in anderen Losungsmitteln gefundenen Werten zu vergleichen. Es zeigt sich, das die Geschwindigkeitskonstanten der Monomeraddition an das Kontaktionenpaar, das solvatgetrennte Ionenpaar und das freie Anion, die sich um Grosenordnungen unterscheiden, vom Losungsmittel nur geringfugig beeinflust werden. Der ungewohnlich grose Einflus der Losungsmittel auf die Polymerisationsgeschwindigkeit geschieht durch Verschiebung der Gleichgewichte zwi…