Search results for "Array"

From a multivariate spatio-temporal array to a multipollutant - multisite Air Quality Index

2010

AQIs are computed on air pollution data that are usually collected according to time, space and type of pollutant: in a given town/region, data consisting of hourly levels of K pollutants recorded in S monitoring sites, are usually organized in a three-mode array. A first aggregation step usually concerns time, and allows to pass from hourly data to a daily synthesis: in this paper data will be aggregated by time according to the guidelines provided by the national agencies producing the three mode array X. Here we will propose a new approach to get a Multipollutant-Multisite Air Quality Index time series from a multivariate spatio-temporal array. This implies a two step aggregation, accord…

Upper and lower generalized factoraggregations based on fuzzy equivalence relation

2014

We develop the concept of a general factoraggre-gation operator introduced by the authors on the basis of an equivalence relation and applied in two recent papers for analysis of bilevel linear programming solving parameters. In the paper this concept is generalized by using a fuzzy equivalence relation instead of the crisp one. By using a left-continuous t-norm and its residuum we define and investigate two modifications of such generalized construction: upper and lower generalized factoraggregations. These generalized factoraggregations can be used for construction of extensional fuzzy sets.

Evolutionary History and Functional Characterization of the Amphibian Xenosensor CAR

2011

AbstractThe xenosensing constitutive androstane receptor (CAR) is widely considered to have arisen in early mammals via duplication of the pregnane X receptor (PXR). We report that CAR emerged together with PXR and the vitamin D receptor from an ancestral NR1I gene already in early vertebrates, as a result of whole-genome duplications. CAR genes were subsequently lost from the fish lineage, but they are conserved in all taxa of land vertebrates. This contrasts with PXR, which is found in most fish species, whereas it is lost from Sauropsida (reptiles and birds) and plays a role unrelated to xenosensing in Xenopus. This role is fulfilled in Xenopus by CAR, which exhibits low basal activity a…

Architecture of a digital PFM controller for IC implementation

2006

This paper presents a digital controller architecture oriented to IC implementation. The classical digital pulse width modulator (D-PWM), using digital analog converter (DAC), is replaced with a Sigma-Delta (/spl Sigma//spl Delta/) modulator based on pulse frequency modulator (PFM) technique. Results of an investigation from a prototype for DC-DC converter, in terms of simulated and experimental performances, are reported, together with harmonic frequency investigation. The control function design is implemented on a field programmable gate array (FPGA). As a consequence of good agreement between simulated and experimental results, the proposed architecture realizes a digital control loop w…

Multiplexed plasmon sensor for rapid label-free analyte detection.

2013

Efficient and cost-effective multiplexed detection schemes for proteins in small liquid samples would bring drastic advances to fields like disease detection or water quality monitoring. We present a novel multiplexed sensor with randomly deposited aptamer functionalized gold nanorods. The spectral position of plasmon resonances of individual nanorods, monitored by dark-field spectroscopy, respond specifically to different proteins. We demonstrate nanomolar sensitivity, sensor recycling, and the potential to upscale to hundreds or thousands of targets.

Plasmonic Nanosensor Array for Multiplexed DNA-based Pathogen Detection

2019

In this research we introduce a plasmonic nanoparticle based optical biosensor for monitoring of molecular binding events. The sensor utilizes spotted gold nanoparticle arrays as sensing platform. The nanoparticle spots are functionalized with capture DNA sequences complementary to the analyte (target) DNA. Upon incubation with the target sequence, it will bind on the respectively complementary functionalized particle spot. This binding changes the local refractive index, which is detected spectroscopically as the resulting changes of the localized surface plasmon resonance (LSPR) peak wavelength. In order to increase the signal, a small gold nanoparticle label is introduced. The binding ca…

Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis

2010

Multivariate science based calibration (SBC) has been applied to the resolution of overlapped peaks in liquid chromatography with diode array detection (LC-DAD). Complex river water samples spiked with 11 pharmaceutical substances resulted in poorly resolved chromatograms containing additional peaks from interfering matrix compounds and a change in the background absorbance due to the mobile phase gradient. Applying the present multivariate approach it was possible to resolve all 11 analytes from overlapping peaks, obtaining linear calibration lines (R2 > 0.96). Recovery percentages on spiked samples ranged between 74.6 and 113.5%, which are quite satisfactory taking into account the low co…

Receptor Activator of NF-kB (RANK) Expression in Primary Tumors Associates with Bone Metastasis Occurrence in Breast Cancer Patients

2011

Background\ud Receptor activator of NFkB (RANK), its ligand (RANKL) and the decoy receptor of RANKL (osteoprotegerin, OPG) play a pivotal role in bone remodeling by regulating osteoclasts formation and activity. RANKL stimulates migration of RANK-expressing tumor cells in vitro, conversely inhibited by OPG.\ud \ud Materials and Methods\ud We examined mRNA expression levels of RANKL/RANK/OPG in a publicly available microarray dataset of 295 primary breast cancer patients. We next analyzed RANK expression by immunohistochemistry in an independent series of 93 primary breast cancer specimens and investigated a possible association with clinicopathological parameters, bone recurrence and surviv…

DNA MICROARRAY AND BIOINFORMATICS AS TOOLS TO IDENTIFY A COMMON MOLECULAR SIGNATURE SHARED BY HUMAN ANEUPLOID CELLS

Genomic instability is a hallmark of the majority of human tumors explaining the heterogeneity shown by tumor cells. This phenomenon is often associated with chromosomal instability (CIN) and aneuploidy, a condition in which tumor cells lose or gain chromosomes. Previously, we showed that posttranscriptional silencing by RNAi of pRb(1), DNMT1(2) and MAD2(3) is associated with aneuploidy in cultured human cells reinforcing the idea that there are several roads leading to aneuploidy. In the attempt to understand if a common molecular signature exists that underlies aneuploidy and its tolerance in tumor cells, we did post transcriptional silencing of Rb, MAD2 and DNMT1 in human fibroblasts (IM…

Mast Cells and Th17 Cells Contribute to the Lymphoma-Associated Pro-Inflammatory Microenvironment of Angioimmunoblastic T-Cell Lymphoma

2010

Reports focusing on the immunological microenvironment of peripheral T-cell lymphomas (PTCL) are rare. Here we studied the reciprocal contribution of regulatory (Treg) and interleukin-17-producing (Th17) T-cells to the composition of the lymphoma-associated microenvironment of angioimmunoblastic T-cell lymphoma (AITL) and PTCL not otherwise specified on tissue microarrays from 30 PTCLs not otherwise specified and 37 AITLs. We found that Th17 but not Treg cells were differently represented in the two lymphomas and correlated with the amount of mast cells (MCs) and granulocytes, which preferentially occurred in the cellular milieu of AITL cases. We observed that MCs directly synthesized inter…