Search results for "Bin"
showing 10 items of 14592 documents
Solanum aethiopicum gr. gilo and Its Interspecific Hybrid with S. melongena as Alternative Rootstocks for Eggplant: Effects on Vigor, Yield, and Frui…
2019
Grafting is generally considered effective in ameliorating vegetable crop tolerance to biotic and abiotic stresses. The use of interspecific hybrid as rootstock for eggplant may represent a valid alternative approach to enhance eggplant performance. However, studies on the effects of different rootstocks on eggplant plant vigor, yield, and fruit quality traits often show conflicting results. Thus, an experiment was performed in two spring&ndash
Resistance of a recombinant Escherichia coli to dehydration.
2009
International audience; Dehydration of microorganisms, rendering them anhydrobiotic, is often an efficient method for the short and long term conservation of different strain-producers. However, some biotechnologically important recombinant bacterial strains are extremely sensitive to conventional treatment. We describe appropriate conditions during dehydration of the recombinant Escherichia coli strain HB 101 (GAPDH) that can result dry cells having a 88% viability on rehydration. The methods entails air-drying after addition of 100 mM trehalose to the cultivation medium or distilled water (for short term incubation).
Molecular Bases for Sensitivity to Tubulin-Binding Herbicides in Green Foxtail
2004
Abstract We investigated the molecular bases for resistance to several classes of herbicides that bind tubulins in green foxtail (Setaria viridis L. Beauv.). We identified two α- and two β-tubulin genes in green foxtail. Sequence comparison between resistant and sensitive plants revealed two mutations, a leucine-to-phenylalanine change at position 136 and a threonine-to-isoleucine change at position 239, in the gene encoding α2-tubulin. Association of mutation at position 239 with herbicide resistance was demonstrated using near-isogenic lines derived from interspecific pairings between green foxtail and foxtail millet (Setaria italica L. Beauv.), and herbicide sensitivity bioassays combine…
Efficient estimation of generalized linear latent variable models.
2019
Generalized linear latent variable models (GLLVM) are popular tools for modeling multivariate, correlated responses. Such data are often encountered, for instance, in ecological studies, where presence-absences, counts, or biomass of interacting species are collected from a set of sites. Until very recently, the main challenge in fitting GLLVMs has been the lack of computationally efficient estimation methods. For likelihood based estimation, several closed form approximations for the marginal likelihood of GLLVMs have been proposed, but their efficient implementations have been lacking in the literature. To fill this gap, we show in this paper how to obtain computationally convenient estim…
Evidence for specific, high-affinity binding sites for a proteinaceous elicitor in tobacco plasma membrane
1995
Abstract Binding of cryptogein, a proteinaceous elicitor, was studied on tobacco plasma membrane. The binding of the [125I]cryptogein was saturable, reversible and specific with an apparent Kd of 2 nM. A single class of cryptogein binding sites was found with a sharp optimum pH for binding at about pH 7.0. The high-affinity correlates with cryptogein concentrations required for biological activity in vivo.
Solving the Puzzle of Taxonomic Position of the Petroleum Fly by Resurrection of Diasemocera Bezzi from Psilopa Fallén (Diptera: Ephydridae) with Pro…
2018
Diasemocera Bezzi, 1895 (Type species: Psilopa nigrotaeniata Bezzi, 1895 [= Psilopa roederi Girschner, 1889]) is resurrected from synonymy with Psilopa and the following four new synonyms of Diasemocera are proposed: Domina Hutton (Type species: Domina metallica Hutton), Discocerinella Mercier (Type species: Discocerinella omonvillea Mercier, 1927 [= Notiphila pulicaria Haliday, 1839]), Trimerinoides Cresson (Type species: Trimerina adfinis Cresson) and Helaeomyia Cresson, 1941 (Type species: Psilopa petrolei Coquillett). Diasemocera is placed close to the genus Trimerina and it differs from the highly specialized Psilopa and related genera by the number of pseudotracheae (6–7), vestiture o…
DNA polymorphism at the FRIGIDA gene in Arabidopsis thaliana : extensive nonsynonymous variation is consistent with local selection for flowering time
2002
FRIGIDA (FRI) is a major gene involved in the regulation of flowering time in Arabidopsis thaliana. Nucleotide variation at this gene was investigated by sequencing 25 field ecotypes collected from western Europe. Genetic diversity at FRI was characterized by a high number of haplotypes and an excess of low-frequency polymorphisms. A large excess of intraspecific nonsynonymous variation associated with low synonymous variation was detected along the first exon in the FRI gene. In contrast, no excess of nonsynonymous divergence was detected between A. thaliana and A. lyrata. The Tajima and McDonald and Kreitman tests, however, suggested that this gene has evolved in a nonneutral fashion. Non…
Characterization of (3H) acifluorfen binding to purified pea etioplasts, and evidence that protoporphyrinogen oxidase specifically binds acifluorfen
1992
It is now generally accepted that protoporphyrinogen oxidase is the target-enzyme for diphenylether-type herbicides. Recent studies [Camadro, J-M., Matringe, M., Scalla, R. & Labbe, P. (1991) Biochem. J. 277, 17–21] have revealed that in maize, diphenyl ethers competitively inhibit protoporphyrinogen oxidase with respect to its substrate, protoporphyrinogen IX. In this study, we show that, in purified pea etioplast, [3H]acifluorfen specifically binds to a single class of high-affinity binding sites with an apparent dissociation constant of 6.2 ± 1.3 nM and a maximum density of 29 ± 5 nmol/g protein. [3H]Acifluorfen binding reaches equilibrium in about 1 min at 30°C. Half dissociation occurs…
Molecular Bases for Sensitivity to Acetyl-Coenzyme A Carboxylase Inhibitors in Black-Grass
2005
Abstract In grasses, residues homologous to residues Ile-1,781 and Ile-2,041 in the carboxyl-transferase (CT) domain of the chloroplastic acetyl-coenzyme A (CoA) carboxylase (ACCase) from the grass weed black-grass (Alopecurus myosuroides [Huds.]) are critical determinants for sensitivity to two classes of ACCase inhibitors, aryloxyphenoxypropionates (APPs) and cyclohexanediones. Using natural mutants of black-grass, we demonstrated through a molecular, biological, and biochemical approach that residues Trp-2,027, Asp-2,078, and Gly-2,096 are also involved in sensitivity to ACCase inhibitors. In addition, residues Trp-2,027 and Asp-2,078 are very likely involved in CT activity. Using three-…
Comparison of binding properties and early biological effects of elicitins in tobacco cells
1998
Abstract Elicitins are a family of small proteins secreted by Phytophthora species that have a high degree of homology and elicit defense reactions in tobacco (Nicotiana tabacum). They display acidic or basic characteristics, the acidic elicitins being less efficient in inducing plant necrosis. In this study we compared the binding properties of four elicitins (two basic and two acidic) and early-induced signal transduction events (Ca2+ influx, extracellular medium alkalinization, and active oxygen species production). The affinity for tobacco plasma membrane-binding sites and the number of binding sites were similar for all four elicitins. Furthermore, elicitins compete with one another fo…