Search results for "Biomedical"
showing 10 items of 2328 documents
Thermodynamic and Kinetic Modeling of Co-utilization of Glucose and Xylose for 2,3-BDO Production by Zymomonas mobilis
2021
Prior engineering of the ethanologen Zymomonas mobilis has enabled it to metabolize xylose and to produce 2,3-butanediol (2,3-BDO) as a dominant fermentation product. When co-fermenting with xylose, glucose is preferentially utilized, even though xylose metabolism generates ATP more efficiently during 2,3-BDO production on a BDO-mol basis. To gain a deeper understanding of Z. mobilis metabolism, we first estimated the kinetic parameters of the glucose facilitator protein of Z. mobilis by fitting a kinetic uptake model, which shows that the maximum transport capacity of glucose is seven times higher than that of xylose, and glucose is six times more affinitive to the transporter than xylose.…
Amine functionalized ZrO2 nanoparticles as biocompatible and luminescent probes for ligand specific cellular imaging
2015
Surface functionalized ZrO2 nanoparticles show strong photoluminescence and are a versatile tool for cellular targeting due to their chemical functionality. They are highly photostable, biocompatible and amenable to coupling with bioligands (e.g. secondary goat anti-rabbit antibody (GAR) and tri-phenyl phosphine (TPP)) via carbodiimide chemistry. Antibody (GAR) functionalized ZrO2 nanoparticles were used to image the nuclear protein Sirt6, whereas triphenyl phosphonium ion (TPP) functionalized ZrO2 nanoparticles specifically targeted the mitochondria. The versatility and easiness of the ZrO2 surface modification opens up new possibilities for designing non-toxic water dispersible and photos…
Model Free Control for muscular force by Functional Electrical Stimulation using pulse width modulation
2016
International audience; Functional Electrical Stimulation (FES) is a useful technique for restoring functions for patients with neurological disorders. Muscle activities can be artificially driven through delivery of electrical pulses to skeletal muscles. Typically, muscles are activated by using constant stimulation train with a fixed parameters (amplitude, frequency and pulse width). In addition, the FES systems do not adapt the parameters stimulation to obtain a desired force response during the rehabilitation session. The purpose of this study is to investigate a real-time FES system for adapting automatically the stimulation parameters (stimulation pulse width) to track a desired force…
Remote Photoplethysmography Based on Implicit Living Skin Tissue Segmentation
2016
International audience; Region of interest selection is an essential part for remote photoplethysmography (rPPG) algorithms. Most of the time, face detection provided by a supervised learning of physical appearance features coupled with skin detection is used for region of interest selection. However, both methods have several limitations and we propose to implicitly select living skin tissue via their particular pulsatility feature. The input video stream is decomposed into several temporal superpixels from which pulse signals are extracted. Pulsatility measure for each temporal superpixel is then used to merge pulse traces and estimate the photoplethysmogram signal. This allows to select …
Parameter-free adaptive step-size multiobjective optimization applied to remote photoplethysmography
2018
International audience; In this work, we propose to reformulate the objective function of Independent Component Analysis (ICA) to make it a better posed problem in the context of Remote photoplethysmography (rPPG). In recent previous works, linear combination coefficients of RGB channels are estimated maximizing the non-Gaussianity of ICA output components. However, in the context of rPPG a priori knowledge of the pulse signal can be incorporated into the component extraction algorithm. To this end, the contrast function of regular ICA is extended with a measure of periodicity formulated using autocorrelation. This novel semi-blind source extraction method for measuring rPPG has the interes…
Automatic detection of P, QRS and T patterns in 12 leads ECG signal based on CWT
2016
International audience; In this paper, a new method based on the continuous wavelet transform is described in order to detect the QRS, P and T waves. QRS, P and T waves may be distinguished from noise, baseline drift or irregular heartbeats. The algorithm, described in this paper, has been evaluated using the Computers in Cardiology (CinC) Challenge 2011 database and also applied on the MIT-BIH Arrhythmia database (MITDB). The data from the CinC Challenge 2011 are standard 12 ECG leads recordings with full diagnostic bandwidth compared to the MITDB which only includes two leads for each ECG signal. Firstly, our algorithm is validated using fifty 12 leads ECG samples from the CinC collection…
Representing and Reasoning for Spatiotemporal Ontology Integration
2004
International audience; The World-Wide Web hosts many autonomous and heterogeneous information sources. In the near future each source may be described by its own ontology. The distributed nature of ontology development will lead to a large number of local ontologies covering overlapping domains. Ontology integration will then become an essential capability for effective interoperability and information sharing. Integration is known to be a hard problem, whose complexity increases particularly in the presence of spatiotemporal information. Space and time entail additional problems such as the heterogeneity of granularity used in representing spatial and temporal features. Spatio-temporal ob…
Toward the development of a remote photoplethysmographic sensor
2019
Heart-rate estimation performed with remote photoplethysmography is a very active research field. Since pioneer works in 2010, which demonstrated the feasibility of the measure with low-grade consumers’ camera (webcam), the number of scientific publications have increased significantly in the domain. Hence, we observe a multiplication of the methods in order to retrieve the photoplethysmographic signal which hasled to an increased precision and quality of the heartrate estimation. Region of interest segmentation is a key step of the processing pipeline in order to maximize the quality of the measured signal. We propose a new method to perform remote photoplethysmographic measurement using a…
Left-Ventricle Segmentation of SPECT Images of Rats
2015
Single-photon emission computed tomography (SPECT) imaging of the heart is helpful to quantify the left-ventricular ejection fraction and study myocardial perfusion scans. However, these evaluations require a 3-D segmentation of the left-ventricular wall on each phase of the cardiac cycle. This paper presents a fast and interactive graph cut method for 3-D segmentation of the left ventricle (LV) of rats in SPECT images. The method is carried out in three steps. First, 3-D sampling of the LV cavity is made in a spherical-cylindrical coordinate system. Then, a graph-cut-based energy minimization procedure provides delineation of the myocardium centerline surface. From there, it is possible to…
Modular Assembly of Multimodal Imaging Agents through an Inverse Electron Demand Diels–Alder Reaction
2019
International audience; The combination of two imaging probes on a same biomolecule gives access to targeted bimodal imaging agents that can provide more accurate diagnosis, complementary information, or that may be used in different applications, such as PET imaging and fluorescence imagingassisted surgery. In this study, we demonstrate that dichlorotetrazine, a small, commercially available compound, can be used as a modular platform to easily assemble various imaging probes. Doubly-labeled tetrazines can then be conjugated to a protein through a biorthogonal IEDDA reaction. A series of difunctionalized tetrazine compounds containing various chelating agents and fluorescent dyes was synth…