Search results for "Calcium"
showing 10 items of 1740 documents
Effects of plant type and calcium cyanamide dosage on yield, nutritional and nutraceutical traits of strawberry in Sicily
2021
In the Mediterranean area, strawberry is usually set up by using bare-rooted plants, fresh plug plants or cold-stored plants, achieving different fruit ripening earliness and crop performance. Strawberry plants can be combined with various cultivation practices to improve strawberry fruit production and quality. Calcium cyanamide (CaCN2) is well-acknowledged as a fertilizer used in the horticulture sector. Its use can enhance the soil fertility, prevent soil acidification, and improve crop production and fruit quality. This work evaluated the effects of different pre-transplant CaCN2 dosages on bare-rooted plants, fresh plug plants or cold-stored plants. Coldstored plants with 400 kg ha(-1)…
Comparative Surface Morphology, Chemical Composition, and Cytocompatibility of Bio-C Repair, Biodentine, and ProRoot MTA on hDPCs
2020
Biocompatibility is an essential property for any vital pulp material that may interact with the dental pulp tissues. Accordingly, this study aimed to compare the chemical composition and ultrastructural morphology of Biodentine (Septodont, Saint Maur-des-Fosses, France), ProRoot MTA (Dentsply Tulsa Dental Specialties, Johnson City, TN, USA), and Bio-C Repair (Angelus, Londrina, PR, Brazil), as well as their biological effects on human dental pulp cells. Chemical element characterization of the materials was undertaken using scanning electron microscopy and energy dispersive X-ray analysis (SEM-EDX). The cytotoxicity was assessed by analyzing the cell viability (MTT assay), cell morphology …
Spine and test skeletal matrices of the Mediterranean sea urchinArbacia lixula- a comparative characterization of their sugar signature
2015
15 pages; International audience; Calcified structures of sea urchins are biocomposite materials that comprise a minor fraction of organic macromolecules, such as proteins, glycoproteins and polysaccharides. These macromolecules are thought to collectively regulate mineral deposition during the process of calcification. When occluded, they modify the properties of the mineral. In the present study, the organic matrices (both soluble and insoluble in acetic acid) of spines and tests from the Mediterranean black sea urchin Arbacia lixula were extracted and characterized, in order to determine whether they exhibit similar biochemical signatures. Bulk characterizations were performed by mono-di…
Ionic currents ofdrosophila embryonic neurons derived from selectively cultured CNS midline precursors
2000
In order to investigate the electrogenesis of defined cell populations, we applied an in vitro system that allows the selective culturing of individual Drosophila CNS precursors under different conditions. CNS midline (ML) precursors prepared from gastrula stage embryos gave rise to progeny cells with neuronal and glial morphology that expressed specific markers. Using whole-cell patch-clamp recordings, a detailed description of ionic currents present in this defined cell population is provided. Most ionic currents of cultured ML neurons were similar to other cultured Drosophila neurons, even though their embryonic origin is different. They displayed at least two voltage-gated potassium cur…
Localization of parvalbumin, calretinin, and calbindin D-28k in identified extraocular motoneurons and internuclear neurons of the cat
1998
Calcium-binding proteins have been shown to be excellent markers of specific neuronal populations. We aimed to characterize the expression of calcium-binding proteins in identified populations of the cat extraocular motor nuclei by means of immunohistochemistry against parvalbumin, calretinin, and calbindin D-28k. Abducens, medial rectus, and trochlear motoneurons were retrogradely labeled with horseradish peroxidase from their corresponding muscles. Oculomotor and abducens internuclear neurons were retrogradely labeled after horseradish peroxidase injection into either the abducens or the oculomotor nucleus, respectively. Parvalbumin staining produced the highest density of immunoreactive …
The endopolygalacturonase 1 from botrytis cinerea activates grapevine defense reactions unrelated to its enzyumatic activity
2003
A purified glycoprotein from Botrytis cinerea(strain T4), identified as endopolygalacturonase 1 (T4BcPG1) by mass spectrometry analysis, has been shown to activate defense reactions in grapevine (Vitis vinifera cv. Gamay). These reactions include calcium influx, production of active oxygen species, activation of two mitogen-activated protein kinases, defense gene transcript accumulation, and phytoalexin production. Most of these defense reactions were also activated in grapevine in response to purified oligogalacturonides (OGA) with a degree of polymerization of 9 to 20. In vivo, these active OGA might be a part of the released products resulting from endopolygalacturonase activity on plan…
Nuclear calcium signaling by inositol trisphosphate in GH3 pituitary cells
2008
It has been proposed that nuclear and cytosolic Ca2+ ([Ca2+]N and [Ca2+]C) may be regulated independently. We address here the issue of whether inositol trisphosphate (IP3) can, bypassing changes of [Ca2+]C, produce direct release of Ca2+ into the nucleoplasm. We have used targeted aequorins to selectively measure and compare the changes in [Ca2+]C and [Ca2+]N induced by IP3 in GH3 pituitary cells. Heparin, an IP3 inhibitor that does not permeate the nuclear pores, abolished the [Ca2+]C peaks but inhibited only partly the [Ca2+]N peaks. The permeant inhibitor 2-aminoethoxy-diphenyl-borate (2-APB) blocked both responses. Removal of ATP also inhibited more strongly the [Ca2+]C than [Ca2+]N pe…
Some aspects related to stability, critical concentrations and kinetics of flocculation of the calcium phytate colloid
1986
Abstract As 99mTcCa phytate is an important radiopharmaceutical and its colloidal nature presents problems, we investigated some of them. This work describes the study of the colloidal behaviour of the calcium phytate colloid in terms of its formation, stability and kinetics of flocculation. The study of spontaneous, and centrifugation-induced flocculation allows the determination of two critical concentrations of sol flocculation. The titrations of calcium phytate colloid at different concentrations provide information on the colloidal formation conditions. Moreover, a study on flocculation kinetics was made by turbidity measurements.
The Effect of High Parathyroid Hormone Concentration on Calcitonin in Patients with Primary Hyperparathyroidism2)
2009
Serum calcitonin (CT), parathyroid hormone (PTH), and calcium levels were measured in 23 patients with primary hyperparathyroidism. PTH was determined by a midregion (M-RIA) and a carboxyl-terminal (C-RIA) specific PTH-RIA. Only 2 patients had elevated CT levels. In contrast to the findings in 46 healthy controls, the CT levels did not correlate with calcium levels. Patients who had the highest iPTH values showed a negative correlation between CT and iPTH (M-RIA (n = 7): R = -1.0000, p less than 0.001; C-RIA (n = 13): R = -0.5604, p less than 0.05). The results of the C-RIA were subtracted from those of the M-RIA. In 12 patients with the highest levels of intact PTH (M-RIA - C-RIA), serum P…
Electron microscopic immunogold cytochemistry reveals chromogranin A confined to secretory granules of porcine Merkel cells
1990
By ultrastructural immunohistochemistry using the immunogold technique, immunoreactive (ir) Chromogranin A (CGA) was found to be confined to the secretory vesicles of porcine Merkel cells. CGA was present predominantly in the periphery of the electron-dense core and on the clear halo. These findings indicate that CGA is a regular constituent of Merkel cell secretory granules but probably not exclusively responsible for their electron opacity.