Search results for "Caps"
showing 10 items of 745 documents
Secretion of the rotavirus VP8* protein inLactococcus lactis
2001
Secretion of the VP8* subunit of the VP4 capsid protein of rotavirus by Lactococcus lactis has been achieved. For this purpose, a secretion vector has been constructed with the lactococcal signal sequence AL9 and the VP8*-encoding gene fragment. The amount of VP8* secreted by L. lactis in the culture supernatant was quantified and visualised by Western blot. Furthermore, it was shown to retain its hemagglutination capability, indicating that the conformation of the secreted peptide may be retaining its biological activity.
The crystal structure of bacteriophage Qβ at 3.5 å resolution
1996
Abstract Background: The capsid protein subunits of small RNA bacteriophages form a T=3 particle upon assembly and RNA encapsidation. Dimers of the capsid protein repress translation of the replicase gene product by binding to the ribosome binding site and this interaction is believed to initiate RNA encapsidation. We have determined the crystal structure of phage Qβ with the aim of clarifying which factors are the most important for particle assembly and RNA interaction in the small phages. Results The crystal structure of bacteriophage Qβ determined at 3.5 a resolution shows that the capsid is stabilized by disulfide bonds on each side of the flexible loops that are situated around the fi…
Spatially segregated transmission of Co-occluded baculoviruses limits virus–virus interactions mediated by cellular coinfection during primary infect…
2022
This article belongs to the Section General Virology.
First report of Antigastra catalaunalis on sesame in Greece.
2017
Τον Αύγουστο του 2016, καταγράφηκαν σοβαρές προσβολές σε καλλιέργειες σουσαμιού από το έντομο Antigastra catalaunalis (Duponchel) (Lepidoptera: Crambidae) σε αγροτικές περιοχές της Περιφερειακής Ενότητας Δράμας. Το A. catalaunalis είναι ένας σημαντικός εχθρός της καλλιέργειας του σουσαμιού σε περιοχές όπου αυτό καλλιεργείται. Παρατηρήθηκαν συμπτώματα προσβολής από τις προνύμφες του εντόμου σε φύλλα, βλαστούς και κάψες. Οι προσβολές καταγράφηκαν σε όλους τους αγρούς με καλλιέργεια σουσαμιού που επιθεωρήθηκαν, με επίπεδα προσβολής υψηλά τα οποία προκάλεσαν οικονομική απώλεια. Στο βαθμό που γνωρίζουμε, αυτή είναι η πρώτη αναφορά σοβαρών προσβολών σουσαμιού από το Α. catalaunalis στην Ελλάδα.
Pentafluoroethylation of Carbonyl Compounds by HFC-125 via the Encapsulation of the K Cation with Glymes
2021
A simple protocol to overcome the explosive pentafluoroethylation of carbonyl compounds by HFC-125 is described. The use of potassium (K) bases with triglyme or tetraglyme as a solvent safely yield...
Preliminary research on conversion of encapsulated somatic embryos of Citrus reticulata Blanco, cv. Mandardino Tardivo di Ciaculli.
2006
Somatic embryogenesis was obtained through anther culture of Citrus reticulata, cv. Mandarino Tardivo di Ciaculli. The work was carried out to evaluate the response of somatic embryos inside a sodium alginate coating to different storage periods, and to the effects of the germicide PPM (1 ml l(-1) stop) and the fungicide Thiophanate-methyl (100 mg l(-1) stop). The effect of these alone or in combination, added to the artificial endosperm on the performance of the encapsulated somatic embryos was tested. After 45 days of culturing, sprouting, root development and conversion were recorded. The encapsulated somatic embryos can be conveniently stored at 4 degrees C, but not for a period longer …
Efficient DNA Packaging of Bacteriophage PRD1 Requires the Unique Vertex Protein P6
2007
ABSTRACT The assembly of bacteriophage PRD1 proceeds via formation of empty procapsids containing an internal lipid membrane, into which the linear double-stranded DNA genome is subsequently packaged. The packaging ATPase P9 and other putative packaging proteins have been shown to be located at a unique vertex of the PRD1 capsid. Here, we describe the isolation and characterization of a suppressor-sensitive PRD1 mutant deficient in the unique vertex protein P6. Protein P6 was found to be an essential part of the PRD1 packaging machinery; its absence leads to greatly reduced packaging efficiency. Lack of P6 was not found to affect particle assembly, because in the P6-deficient mutant infecti…
Chemometric determination of arsenic and lead in untreated powdered red paprika by diffuse reflectance near-infrared spectroscopy.
2008
It has been evaluated the potential of near-infrared (NIR) diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) as a way for non-destructive measurement of trace elements at microg kg(-1) level in foods, with neither physical nor chemical pre-treatment. Predictive models were developed using partial least-square (PLS) multivariate approaches based on first-order derivative spectra. A critical comparison of two spectral pre-treatments, multiplicative signal correction (MSC) and standard normal variate (SNV) was also made. The PLS models built after using SNV provided the best prediction results for the determination of arsenic and lead in powdered red paprika samples. Relativ…
Observations on a new species of Unicapsula (Myxosporida) in the fish Maena smaris (L.) by conventional and electronmicroscopy
1975
A myxosporidian parasite believed to be congeneric with Unicapsula muscularis Davis (1924), type (and only) species of this genus, was found in the dorsal muscles of the fish Maena smaris (L.) at Banyuls-sur-Mer, France. The spores were tripartite, with corresponding components of the three parts differentially developed. One part consisted of a small shell valve overlying no more than a trace of sporoplasm but covering a polar apparatus with well-developed polar capsule. Each of the other two parts consisted of a long shell valve covering a large sporoplasm and a polar apparatus with only the rudiment of a polar capsule. Electron microscope pictures of the trophozoite showed evidence of pi…