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showing 10 items of 14808 documents
Membrane-Associated Enteroviruses Undergo Intercellular Transmission as Pools of Sibling Viral Genomes
2019
Summary Some viruses are released from cells as pools of membrane-associated virions. By increasing the multiplicity of infection (MOI), this type of collective dispersal could favor viral cooperation, but also the emergence of cheater-like viruses such as defective interfering particles. To better understand this process, we examined the genetic diversity of membrane-associated coxsackievirus infectious units. We find that infected cells release membranous structures (including vesicles) that contain 8–21 infectious particles on average. However, in most cases (62%–93%), these structures do not promote the co-transmission of different viral genetic variants present in a cell. Furthermore, …
Arthroscopic Reinsertion of Acute Injuries of the Scapholunate Ligament Technique and Results
2020
Abstract Objective To describe a technique for treating acute injuries of the scapholunate ligament (SLL) by performing an arthroscopic reinsertion of the SLL and dorsal capsulodesis and to present the results obtained. Methods The study deals with an analytical, prospective clinical study that included 19 consecutive patients with acute injury of the SLL. The range of joint motion, grip strength, pain according to the visual analog scale, functional outcomes according to the Mayo Wrist Score (MWS), and the QuickDASH Score were studied preoperatively and 6 and 12 months postoperatively. The complications and necessary reinterventions were recorded. Results Nineteen patients with acute injur…
Sustained activation of sphingomyelin synthase by 2-hydroxyoleic acid induces sphingolipidosis in tumor cells
2013
The mechanism of action of 2-hydroxyoleic acid (2OHOA), a potent antitumor drug, involves the rapid and specific activation of sphingomyelin synthase (SMS), leading to a 4-fold increase in SM mass in tumor cells. In the present study, we investigated the source of the ceramides required to sustain this dramatic increase in SM. Through radioactive and fluorescent labeling, we demonstrated that sphingolipid metabolism was altered by a 24 h exposure to 2OHOA, and we observed a consistent increase in the number of lysosomes and the presence of unidentified storage materials in treated cells. Mass spectroscopy revealed that different sphingolipid classes accumulated in human glioma U118 cells af…
Thermostabilization of BSA in TMAO Water Mixtures by Infrared Spectroscopy
2019
Background:Trimethylamine-N-Oxide (TMAO) is a small organic molecule derived from the metabolism of L-carnitine and choline after ingestion of animal food. TMAO has many functions such as electron acceptor, an osmolyte, stabilizer of macromolecules folding. It seems that TMAO plays an important role in nature but, in humans, it is a remnant of the evolution of the osmolyte system.Objective:The present paper is addressed on the study of thermal stability of hydrated Bovine Serum Albumins (BSA) in the presence of water and TMAO water solution by means of InfraRed spectroscopy. In particular, this work has investigated the protein amide I spectral regions, which is sensitive to protein seconda…
Dispersion from Cα or NH: 4D experiments for backbone resonance assignment of intrinsically disordered proteins
2020
AbstractResonance assignment of intrinsically disordered proteins is remarkably challenging due to scant chemical shift dispersion arising from conformational heterogeneity. The challenge is even greater if repeating segments are present in the amino acid sequence. To forward unambiguous resonance assignment of intrinsically disordered proteins, we present iHACANCO, HACACON and (HACA)CONCAHA, three Hα-detected 4D experiments with Cα as an additional dimension. In addition, we present (HACA)CON(CA)NH and (HACA)N(CA)CONH, new 4D Hα-start, HN-detect experiments which have two NH dimensions to enhance peak dispersion in a sequential walk through C′, NH and HN, and provide more accurate NH/HN ch…
Imaging Noncanonical Autophagy and LC3-Associated Phagocytosis in Cultured Cells
2019
International audience; Monitoring of ATG8 proteins by western blotting and immunofluorescence microscopy are the most common methods to monitor the autophagy pathway. However, it has recently been shown that ATG8 proteins can be lipidated to non-autophagosome, single-membrane compartments through a noncanonical autophagy pathway. This is commonly found to occur during macro-endocytic processes such as phagocytosis, where it has been termed LC3-associated phagocytosis, and upon lysosomotropic drug treatment. Therefore, care is required when interpreting data based on ATG8 in order to conclude whether a signal relates to the canonical or noncanonical pathway. Here we provide methods to monit…
Application of FTIR Spectroscopy to Analyze RNA Structure
2020
Fourier transform infrared (FTIR) spectroscopy has been widely used for the analysis of both protein and nucleic acid secondary structure. This is one of the vibration spectroscopy methods that are extremely sensitive to any change in molecular structure. While numerous reports describe how to proceed to analyze protein and deoxyribonucleic acid (DNA) structures using FTIR, reports related to the analyses of ribonucleic acids (RNAs) are few. Nevertheless, RNAs are versatile molecules involved in a multitude of roles in the cell. In this chapter, we present applications of FTIR for the structural analysis of RNA, including the analysis of helical parameters and noncanonical base pairing, oft…
Application of high-content screening for the study of hepatotoxicity: Focus on food toxicology
2020
Safety evaluation of thousands of chemicals that are directly added to or come in contact with food is needed. Due to the central role of the liver in intermediary and energy metabolism and in the biotransformation of foreign compounds, the hepatotoxicity assessment is essential. New approach methodologies have been proposed for the safety evaluation of compounds with the idea of rapidly gaining insight into effects on biochemical mechanisms and cellular processes and screening large number of compounds. In this sense, high-content screening (HCS) is the application of automated microscopy and image analysis for better understanding of complex biological functions and mechanisms of toxicity…
Attosecond Soft-X-Ray Spectroscopy in a Transition Metal Dichalcogenide
2019
Information about the real-time response of carriers to optical fields is paramount to advance information processing or to understand the bottlenecks of light-matter interaction and energy harvesting. Transition-metal dichalcogenide (TMDC) compounds are an emerging class of materials with attractive structural and electronic properties that can be thinned to the 2D limit and TiS 2 is a paradigmatic example for a semi-metallic TMDC, since its electron mobility ranges between that of a metal and a semiconductor.
NMR Exchange Format: a unified and open standard for representation of NMR restraint data
2015
SCOPUS: le.j