Search results for "DNA profiling"
showing 10 items of 64 documents
Mitochondrial DNA in the central european population
2000
Sequencing of mtDNA is an advanced method for the individualisation of traces. Disadvantages of this method are expensive and time-consuming analysis and evaluation procedures as well as the necessary stock of population-genetic data which is still insufficient. Central European institutes of forensic medicine from Germany, Austria, and Switzerland have been working together since the beginning of 1998 to establish a mtDNA database. The aim is to build up a large stock of forensically established data and provide population-genetic data for frequency investigations, which will serve as a basis for expert opinions and scientific research. Good data quality is ensured by using original sequen…
Isolation of the DNA minisatellite probe MZ 1.3 and its application to DNA ‘fingerprinting’ analysis
1990
Abstract A minisatellite probe, MZ 1.3, detecting hypervariable fragment patterns was isolated from a human genomic library. A repetitive sequence of 27 bp length was identified which is contained in the probe approx. 40 times. The MZ 1.3 repeat shows variable homology of 53–73% to the repetitive sequence of the protein III gene of the bacteriophage M13 genome. Polymorphic restriction fragment patterns were found with MZ 1.3 using the enzymes Hinf I, BstN I, Hae III, Mbo I, PstI PvuII , and Rsa I. An average of 18 polymorphic fragments was observed using Hinf I as enzyme. The band sharing frequency after Hinf I digestion among unrelated individuals was determined to be 23.8 ± 7.2%. An examp…
Emerging GII.4 norovirus variants affect children with diarrhea in Palermo, Italy in 2006
2008
Although the genetic/antigenic heterogeneity of human noroviruses (NoVs) is impressive, a few genogroup II strains of genotype 4 (GII.4) are dominant worldwide. GII.4 NoVs evolve rapidly and in the last 15 years six epidemic variants have been identified. In 2005-2006, surveillance of sporadic viral gastroenteritis in children in Palermo, Italy, resulted in the detection of NoV strains in 20.9% of the patients admitted to hospital. By restriction fragment length polymorphism (RFLP) and sequence analysis of region A in the RNA-dependent RNA-polymerase (RdRp) gene, 59 NoV strains were successfully characterized. Eighty-one percent of the strains were characterized as GII.4, 14% as GIIb/Hilver…
Microevolution in the sicilian shrew crocidura sicula (mammalia, soricidae) tested by rapd-pcr fingerprinting
1997
Genetic variation in samples of the endemic Crocidura sicula living in Sicily and in two surrounding small islands, Marettimo and Ustica, was analysed by Random Amplified Polymorphic DNA fingerprinting (RAPD) and compared to morphometrics and external phenotypes. Molecular variation in the random sample of 99 DNA fragments of the Ustica shrews, showing.a melanic fur and a size-shape variation in skull morphometrics, is of comparable size to that of the of northwestern and northeastern samples Sicily (Tufanio and Madonie). In the Marettimo shrews, bicoloured (grey and white) animals like those coming from Sicily and presenting a significant reduction in body-size and skull morphometrics, mol…
Non-isotopic DNA fingerprint analyses with the minisatellite probe MZ1.3
1990
RFLP analyses with minisatellite probes yield highly informative individual specific banding patterns (genetic fingerprints) (Jeffreys et al.). Because of the complexity of the pattern and marked differences in band intensities a good band resolution and high sensitivity of the probe are essential. By reason of the latter requirement to date most of the RFLP-studies are performed with radioactive probes. Although non-isotopic labels have been introduced into fingerprint analyses (Schafer et al.; Medeiros et al.) 32P-labeled probes are still superior with respect to sensitivity. Our approach to increase the specific signal intensity makes use of an amplification of the number of probe molecu…
The EDNAP mitochondrial DNA population database (EMPOP) collaborative exercises: organisation, results and perspectives.
2003
This paper presents an overview of the organisation and the results of the collaborative exercises (CE) of the European DNA Profiling (EDNAP) Group's mitochondrial DNA population database project (EMPOP). The aim of the collaborative exercises was to determine whether uniformity of mtDNA sequencing results could be achieved among different laboratories. These were asked to sequence either the complete mtDNA control region or the two hypervariable regions HVI (16024-16365) and HVII (73-340) from DNA extracts, buccal swabs or bloodstains, proceeding in accordance with the protocol and strategies used in each individual laboratory. The results of the collaborative exercises were employed to id…
Biochemistry and molecular biology techniques for person characterization
2008
Using the traditional serological tests and the most novel techniques for DNA fingerprinting, forensic scientists scan different traits that vary from person to person and use the data to include or exclude suspects based on matching with the evidence obtained in a criminal case. Although the forensic application of these methods is well known, the procedures and techniques used to obtain these results are not so well studied. Here, we report a laboratory exercise aimed to familiarize the students in several of the methods and markers employed for people individualization. With this exercise, students have to characterize themselves and an unknown sample according to the following character…
A Polyphasic Approach to Study the Intraspecif ic Diversity Amongst Vibrio vulnificus Isolates
1997
Summary A polyphasic taxonomic approach using phenotypic and molecular genetic techniques, was carried out on the species Vibrio vulnificus in order to study its intraspecific diversity. Seven techniques, including phenotypic (API 20E, BIOLOG, total protein profiles, serotyping, ELISA), and genotypic methods (ribotyping and AFLP), were employed on 80 V. vulnificus strains of biotypes 1 and 2, including 9 reference cultures. The isolates came from different geographic origins (USA, Spain, Belgium, Sweden, Norway, Japan, Thailand) and types of samples (clinical, health/diseased fish, seafood, water). Diversity indexes calculated for strains of both biotypes revealed a higher phenotypic and ge…
Likelihood Calculations in Paternity Testing on the Basis of DNA-Fingerprints
1990
DNA-fingerprints seem to be a powerful tool in paternity testing. But the quantification of the results in terms of likelihood and likelihood ratios is a problem, because in most cases the correct genetic model and its parameters are not known. Two approaches have been suggested to circumvent these problems. The use of band sharing rates to distinguish between pairs of relatives and pairs of unrelated individuals, and the calculation of likelihood ratios on the basis of simplifying assumptions. The first approach reduces the available genetic evidence to “phenotypic” similarities. The second one makes unjustified simplifying assumptions. These two decision strategies have to be examined wit…
Protein and DNA fingerprinting of a soil bacterial community inoculated into three different sterile soils
2007
The functional and genetic structures of a soil bacterial community were characterized after inoculation into three different sterile soils using a protein and DNA fingerprinting method, respectively. Principal component analysis (PCA) of profiles revealed that, depending on soil characteristics, bacterial communities with similar genetic structures harbored different functional structures and thus could potentially be of differing ecological significance for soil functioning. Co-inertia analysis between protein fingerprinting data and the corresponding sets of soil physicochemical characteristics demonstrated the correlation between the functional structure of the bacterial community and s…