Search results for "DSR"
showing 10 items of 17 documents
RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design.
2011
International audience; Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involv…
New Insights into the Genome Organization of Yeast Killer Viruses Based on “Atypical” Killer Strains Characterized by High-Throughput Sequencing
2017
Viral M-dsRNAs encoding yeast killer toxins share similar genomic organization, but no overall sequence identity. The dsRNA full-length sequences of several known M-viruses either have yet to be completed, or they were shorter than estimated by agarose gel electrophoresis. High-throughput sequencing was used to analyze some M-dsRNAs previously sequenced by traditional techniques, and new dsRNAs from atypical killer strains of Saccharomyces cerevisiae and Torulaspora delbrueckii. All dsRNAs expected to be present in a given yeast strain were reliably detected and sequenced, and the previously-known sequences were confirmed. The few discrepancies between viral variants were mostly located aro…
Dubultspiralizēto RNS modulējošās īpašības perifēro asiņu mononukleārās šūnās un audzēju šūnās
2016
Dubultspiralizētā RNS (dsRNS) darbojas kā iedzimtās un adaptīvās imūnatbildes aktivators. Larifāns ir Latvijā ražots farmaceitisks produkts, kas satur bakteriofāga izcelsmes dsRNS molekulas. PolyI:C ir viens no plaši pētītiem dsRNS sintētiskajiem analogiem. Bakalaura darba mērķis bija noraksturot dsRNS inducēto citokīnu spektru un limfocītu subpopulācijas ex vivo kultivētās mononukleārās šūnās, kā arī izvērtēt dsRNS ietekmi uz vēža šūnu mitotisko aktivitāti. Abas darbā analizētās dsRNS neizraisīja statistiski būtiskas izmaiņas galvenajās limfocītu populācijās, toties palielināja CD95, CD38, IFN-γ ekspresiju. Novēroja 13 dažādu molekulu indukciju, kā arī IL-16 supresiju Larifāna un PolyI:C k…
Comparison between bitumen modified with crumb tyre rubber and styrene butadiene styrene
2009
The improvements in terms of mechanical properties due to the use of polymers to modify bitumens in road paving applications have already been widely demonstrated. The aim of this paper is to show how the rheology and the performance of a bituminous binder mixed with crumb rubber recycled from used tyres (CR-MB), could be comparable with a styrene butadiene styrene (SBS-MB) created with the same base binder and currently used for road applications. Physical, chemical and performance characterization followed by rheological and storage stability analyses have been undertaken as the basis for the comparison. The results of the investigation indicate that the blended CR-MB shows large improvem…
Arbete eller människorelation? : diskurser om närståendevård under social- och hälsovårdsreformen
2018
Suomessa ikääntyvä yhteiskunta ja hyvinvointivaltion rahoituskriisi haastavat nykyisen hoivapalvelujärjestelmän. Tähän haasteeseen pyritään osittain vastaamaan hallituksen kärkihankkeella, jossa kehitetään koti- ja omaishoitoa. Omaishoito asettuu julkisen ja yksityisen, hoivan ja työn välimaastoon ja se koskettaa erityisesti ikääntyneitä ja vammaisia henkilöitä. Kärkihanke liittyy tiiviisti myös sosiaali- ja terveyspalvelu-uudistukseen, jonka tavoitteena on asiakaslähtöiset ja kustannustehokkaat palvelut. Tässä tutkielmassa tarkastelen omaishoidon diskursseja sote-uudistuksen yhteydessä. Pyrin selvittämään mitä diskursseja julkisessa keskustelussa esiintyy, kuinka ne rakentuvat kielellisest…
Why Should the Q-method be Integrated Into the Design Science Research? A Systematic Mapping Study
2019
The Q-method has been utilized over time in various areas, including information systems. In this study, we used a systematic mapping to illustrate how the Q-method was applied within Information Systems (IS) community and proposing towards the integration of Q-method into the Design Sciences Research (DSR) process as a tool for future research DSR-based IS studies. In this mapping study, we collected peer-reviewed journals from Basket-of-Eight journals and the digital library of the Association for Information Systems (AIS). Then we grouped the publications according to the process of DSR, and different variables for preparing Q-method from IS publications. We found that the potential of t…
Rheological properties of asphalt binder modified with waste polyethylene: An interlaboratory research from the RILEM TC WMR
2022
Funding Information: The RILEM Technical Committee on Valorisation of Waste and Secondary Materials for Roads (TC 279-WMR) and the members of Task Group 1 are gratefully acknowledged. The authors would also like to thank Nynas AB and Swiss company Innorecycling for supporting the interlaboratory activity by providing reference neat binder and PE-pellets, PE-shreds additives. The contribution of the Swiss National Science Foundation ( 205121_178991 ) to the Swiss partners is also acknowledged. Funding Information: The RILEM Technical Committee on Valorisation of Waste and Secondary Materials for Roads (TC 279-WMR) and the members of Task Group 1 are gratefully acknowledged. The authors would…
2′,5′-oligoadenylate synthetase from a lower invertebrate, the marine sponge Geodia cydonium, does not need dsRNA for its enzymatic activity
2002
AbstractRecently, the presence of 2′,5′-linked oligoadenylates and a high 2′,5′-oligoadenylate synthetase activity were discovered in a lower invertebrate, the marine sponge Geodia cydonium. It has been demonstrated that mammalian 2–5A synthetase isozymes require a dsRNA cofactor for their enzymatic activity. Our results show that, unlike mammalian 2–5A synthetases, the 2–5A synthetase from the sponge acts in a dsRNA-independent manner in vitro. A prolonged incubation of the G. cydonium extract with a high concentration of a micrococcal nuclease had no effect on the activity of the 2–5A synthetase. At the same time, the micrococcal nuclease was effective within 30 min in degrading dsRNA nee…
Dubultspiralizēto RNS inducētie citokīni EX VIVO kultivētās perifēro asiņu mononukleārājās šūnās
2015
Dubultspirāliskās RNS (dsRNS) ir plejotropiski eikariotu šūnu iedzimtās un iegūtās imūnas sistēmas regulatori. Larifāns ir dabiskas izcelsmes dsRNS preparāts, kura inducēto citokīnu spektrs ir maz pētīts. Maģistra darba mērķis bija noteikt citokīnu spektru, ko Larifāns inducē mononukleāro šūnu īslaicīgās kultūrās un salīdzināt to ar Poly I:C, kā arī noteikt Larifāna inducētos citokīnus dzemdes kakla displāzijas slimnieču mononukleāro šūnu īslaicīgās kultūrās. Ar Luminex xMAP tehnoloģiju tika raksturota Larifāna un Poly I:C ietekme uz 29 dažādu citokīnu un hemokīnu produkciju. Larifāna un Poly I:C ietekmē atšķirīgā intensitātē tiek inducētas un supresētas vienas un tās pašas molekulas. IL-10…
A new procedure to determine the rheological properties of RAP binder and corresponding bituminous blends
2017
Abstract In this paper, a new and simple approach to estimate the rheological properties of Reclaimed Asphalt Pavement (RAP) binder is proposed to overcome the limitations of the conventional extraction procedure. This method is based on Dynamic Shear Rheometer tests performed on mortars composed of RAP fine fraction and virgin binder, together with a new back-calculation solution. The properties of the bituminous blends of virgin and RAP binders are obtained with a modified Nielsen model. The Voigt model is then successfully used to estimate complex modulus and phase angle of the RAP binder from the properties of the back-calculated binder blend. This procedure would prevent the need of RA…