Search results for "Digital image processing"

Reduction of spherical-aberration impact in microscopy by wavefront coding

2009

In modern high-NA optical scanning instruments, like scanning microscopes, the refractive-index mismatch between the sample and the immersion medium introduces a significant amount of spherical aberration when imaging deep inside the specimen, spreading out the impulse response. Since such aberration depends on the focalization depth, it is not possible to achieve a static global compensation for the whole 3D sample in scanning microscopy. Therefore a depth-variant impulse response is generated. Consequently, the design of pupil elements that increase the tolerance to this aberration is of great interest. In this paper we report a hybrid technique that provides a focal spot that remains alm…

Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope

2022

Lensless holographic microscope (LHM) is an emerging very promising technology that provides high-quality imaging and analysis of biological samples without utilizing any lens for imaging. Due to its small size and reduced price, LHM can be a very useful tool for the point-of-care diagnosis of diseases, sperm assessment, or microfluidics, among others, not only employed in advanced laboratories but also in poor and/or remote areas. Recently, several LHMs have been reported in the literature. However, complete characterization of their optical parameters remains not much presented yet. Hence, we present a complete analysis of the performance of a compact, reduced cost, and high-resolution LH…

Selective Change Driven Imaging: A Biomimetic Visual Sensing Strategy

2011

Selective Change Driven (SCD) Vision is a biologically inspired strategy for acquiring, transmitting and processing images that significantly speeds up image sensing. SCD vision is based on a new CMOS image sensor which delivers, ordered by the absolute magnitude of its change, the pixels that have changed after the last time they were read out. Moreover, the traditional full frame processing hardware and programming methodology has to be changed, as a part of this biomimetic approach, to a new processing paradigm based on pixel processing in a data flow manner, instead of full frame image processing.

A Novel Technique for Monitoring the W-Beam Guardrails

2014

The paper addresses the problem of the measurement of guardrails’ deformations by means automatic analysis of optical images. The technique is founded on the analysis of high-resolution photos, obtained by means of 3D camera installed into a data vehicle. The novel methodology has been used to calculate the longitudinal safety barrier deformations for one W-beam guardrails installed along a rural road in Italy. The procedure has shown that digital image analysis can be used with the aim to monitoring guardrails and, therefore, for guarantee reasonable levels of protection against serious ran-off-road crashes.

Three-Dimensional Flow and Temperature Distribution in Rayleigh-Bènard Convection Using Thermochromic Liquid Crystals and Digital Image Processing

2000

Analysis of the imaging method for assessment of the smile of laser diode bars.

2009

We study imaging systems designed to assess the smile of laser diode bars (LDBs). The magnification matrix is derived from the required sampling period and the geometries of the LDBs and the charge-coupled device (CCD) array. These image-forming systems present in-plane pure translation invariance, but in the case of anamorphic ones, lack in-plane rotation invariance. It is shown that the smile parameters of the image of the LDB are linked with the smile parameters of the LDB by simple mathematical expressions. The spatial resolution of such optical systems is estimated at approximately 1 microm for a mean wavelength of lambda approximately 800 nm. Our results suggest that, with the current…

Four channels multi-illumination single-holographic-exposure lensless Fresnel (MISHELF) microscopy

2018

Abstract MISHELF microscopy [Opt. Express 23, 21352 (2015)] has been recently reported as the background technology of a new concept of compact, cost-effective and field-portable lensless microscope [Sci. Rep. 7, 43291 (2017)] based on wavelength multiplexing and a fast and robust algorithm for twin image minimization and noise reduction. In this manuscript, MISHELF microscopy is expanded beyond its actual configuration by considering 4 illumination/detection channels while retaining its working principle concerning single-shot, twin image mitigation and noise averaging. Proof of principle validation of the proposed improvement is conducted through experiments with a resolution test target …

Three-Dimensional Mapping and Ranging of Objects Using Speckle Pattern Analysis

2010

In this chapter, we present two novel approaches for 3-D object shape measurement and range estimation based on digital image processing of speckle patterns. In the first one, 3-D mapping and range measurement are retrieved by projecting, through a ground glass diffuser, random speckle patterns on the object or on the camera for a transmissive and reflective configuration, respectively. Thus, the camera sensor records in time sequence different speckle patterns at different distances, and by using correlation operation between them, it is possible to achieve 3-D mapping and range finding. In the second one, the 3-D mapping and ranging are performed by sensing the visibility associated with …

Common-path phase-shifting lensless holographic microscopy

2010

We present an approach capable of high-NA imaging in a lensless digital in-line holographic microscopy layout even outside the Gabor's regime. The method is based on spatial multiplexing at the sample plane, allowing a common-path interferometric architecture, where two interferometric beams are generated by a spatial light modulator (SLM) prior to illuminating the sample. The SLM allows phase-shifting interferometry by phase modulation of the SLM diffracted beam. After proper digital processing, the complex amplitude distribution of the diffracted object wavefront is recovered and numerically propagated to image the sample. Experimental results are reported that validate the proposed metho…

Variable zoom digital in-line holographic microscopy

2020

Abstract We report on a novel layout providing variable zoom in digital in-line holographic microscopy (VZ-DIHM). The implementation is in virtue of an electrically tunable lens (ETL) which enables to slightly shift the illumination source axial position without mechanical movement of any system component. Magnifications ranging from ~15X to ~35X are easily achievable using the same layout and resulting in a substantial variation of the total field of view (FOV). The performance of the proposed setup is, first, validated using a resolution test target where the main parameters are analyzed (theoretically and experimentally) and, second, corroborated analyzing biological sample (prostate can…