Search results for "ELISA"
showing 10 items of 169 documents
Divu Helicobacter Pylori asins plazmas testu un fēču antigēnu testa precizitātes izvērtējums pacientiem Latvijas populācijā
2017
Ievads. Populācijās ar augstu Helicobacter pylori (H.pylori) izplatību rekomendē „pārbaudi-un-ārstē” stratēģiju, kur priekšroka ir dodama neinvazīviem testiem, no kuriem ekonomiski izdevīgāki ir antivielu un fēču antigēnu testi. Darba mērķis. Noteikt H. pylori neinvazīvo diagnostisko testu (lateksa-aglutinācijas, ELISA (ar enzīmu saistīta imūnfermentatīvā metode), fēču antigēnu testa) precizitāti, salīdzinot tos ar histoloģiju un noteikt piemērotāko skrīninga metodi H. pylori infekcijas diagnostikai Latvijas populācijā. Materiāli un metodes. Tika analizēti pacientu dati vecumā no 40 līdz 64 gadiem (GISTAR pilotpētījuma ietvaros), kuriem tika piedāvāts veikt ezofagogastroduodenoskopiju. Paci…
Highly sensitive monoclonal antibody-based immunoassays for boscalid analysis in strawberries
2018
Boscalid is an agrochemical recently developed for crop protection and the most significant member of the succinate dehydrogenase inhibitor group of fungicides. In this study, a collection of high-affinity monoclonal antibodies was generated to boscalid. By using a series of haptens with a linker at alternative tethering sites of the boscalid framework, specific antibodies were isolated as well as antibodies that also recognized the main boscalid metabolite. Two immunoassays were developed using different ELISA formats. Optimized assays displayed very high sensitivities (limits of detection were near 0.01 µg/L). Trueness and precision for the determination of the target analyte in strawberr…
Suitability Of Cellular Network Signaling Data For Origin-Destination Matrix Construction: A Case Study Of Lyon Region (France)
2019
TRB 2019, 98th Annual Meeting Transportation Research Board, Washigton, D.C., ETATS-UNIS, 13-/01/2019 - 17/01/2019; Spatiotemporal data, and more specifically origin-destination matrices, are critical inputs to mobility studies for transportation planning and urban management purposes. In this paper, we propose a methodology to infer origin-destination (O-D) matrices based on passively-collected cellular signaling data of millions of anonymized mobile phone users in the Rhône-Alpes region, France. This dataset, which consists of records time-stamped with users' unique identifier and tower locations, is used to first analyze the cell phone activity degree indicators of each user in orde…
Paysage visible et paysage sonore : modélisation intégrée du cadre de vie.
2018
International audience
Valutazione di alcune performance diagnostiche di kit ELISA per la diagnosi sierologica di Anemia Infettiva Equina (AIE)
2012
Data on evaluation of some diagnostic parameters of all ELISA kits available in Italy for the serodiagnosis of AIE are presented and discussed. Ten laboratories were involved using a panel of 30 sera ran with 4 commercial kits and 2 in-house kits. Kits were also evaluated using a panel of sera from vaccinated animals at different days post vaccination (p.v.). All sera were also tested in agar gel immunodiffusion (AGID). The parameters evaluated were: diagnostic sensitivity (DSe) and specificity (DSp), Cohen K, weighted Cohen K, coefficient of variation (CV), accordance, concordance. Analysis of these parameters indicates that all kits have a higher sensitivity than AGID, even if a complete …
Monitoring serologic response to single in ovo vaccination with an immune complex vaccine against infectious bursal disease in broilers
2021
Este artículo se encuentra disponible en la siguiente URL: https://www.sciencedirect.com/science/article/pii/S003257912100033X The infectious bursal disease (IBD) virus is one of the most resistant and prevalent virus worldwide in the poultry industry, being vaccination the main tool to control the disease. For this reason, consistent and uniform immunization of broiler flocks against IBD is necessary to avoid the disease spreading. The aim of this study was to apply and assess an epidemiologic mapping tool focused on the immunization by in ovo single broiler vaccination using an immune complex IBD vaccine. With this regard, 7,576 serum samples were collected from 603 broiler flocks raised …
Optimizing Fuel Consumption and Pollutant Emissions of a Spark Ignition Engine for Eco-driving Applications
2018
VPPC 2018, Vehicle Power and Propulsion Conference, Chicago, ETATS-UNIS, 27-/08/2018 - 30/08/2018; The transportation sector is a major contributor to both air pollution and greenhouse gas emissions. While optimizing fuel consumption reduces CO2 emissions, it can increase fuel-rich operation and cause higher HC and CO emissions. A simplified emissions model is thus introduced in order to account for the impact of air/fuel ratio on both the exhaust concentration of regulated pollutants and the catalyst efficiency. This model is used to solve the eco-driving problem with dynamic programming and a weighted objective function. An emission-centered and a consumption-centered scenario are compare…
Serological biomarkers for the diagnosis of Mycobacterium abscessus infections in cystic fibrosis patients
2021
International audience; Background: Culture conditions sometimes make it difficult to detect non-tuberculous mycobacteria (NTM), particularly Mycobacterium abscessus, an emerging cystic fibrosis (CF) pathogen. The diagnosis of NTM positive cases not detected by classical culture methods might benefit from the development of a serological assay.Methods: As part of a diagnostic accuracy study, a total of 173 sera CF-patients, including 33 patients with M. abscessus positive cultures, and 31 non-CF healthy controls (HC) were evaluated. Four M. abscessus antigens were used separately, comprising two surface extracts (Interphase (INP) and a TLR2 positive extract (TLR2eF)) and two recombinant pro…
Sensitive Monoclonal Antibody-Based Immunoassays for Kresoxim-methyl Analysis in QuEChERS-Based Food Extracts
2014
Kresoxim-methyl is nowadays widely used to combat a diversity of common diseases affecting high-value crops. In this article, we report the development and characterization of two novel immunoassays for the analysis of this pioneer strobilurin fungicide, and for the first time, a validation study with food samples was performed. A direct and an indirect competitive immunoassay based on a new anti-kresoxim-methyl monoclonal antibody were developed for sensitive and specific chemical analysis. Optimized assays showed limits of detection of 0.1 μg/L. Fruit and vegetable samples were extracted with acetonitrile by the QuEChERS procedure and analyzed by the developed immunoassays after a simple …
Evaluation of reverse transcription and polymerase chain reaction (RT/PCR) for the detection of rotaviruses: applications of the assay.
1996
Summary Our aim was to evaluate the reverse transcription and polymerase chain reaction (RT/PCR) technique for the detection of rotavirus shedding by infected children as a routine diagnostic procedure, in comparison to the enzyme-linked immunosorbent assay (ELISA), electron microscopy (EM) and polyacrylamide gel etectrophoresis (PAGE) of rotavirus double stranded RNA. Two-hundred and twenty stool specimens were collected from infants and young children with diarrhoea, and 10–20% faecal suspensions were made. Several methods of rotavirus dsRNA extraction were assayed. Electrophoretic analysis of viral RNA was carried out on 10% polyacrylamide gols followed by silver staining. RT/PCR was per…