Search results for "Editing"
showing 10 items of 94 documents
The plants of the future. : genome editing in biotechnology
2021
The evolution of life has led to the formation of complex systems where plants are essentials. Homo sapiens’ success is based on its ability to obtain food. In what remains of this century, the world population will increase by a quarter of the current total, reaching 10 billion people. This is itself a major challenge, amplified by the environmental conditions resulting from global change and the threat to sustainability derived from the use of the planet’s natural resources. Thus, we wonder if we will be able to respond to this challenge and, to that end, how the plants of the future should look. Recent advances in sequencing techniques allow us to identify genomes at a low cost, and geno…
Biochemical and Immunological implications of Lutein and Zeaxanthin
2021
Throughout history, nature has been acknowledged for being a primordial source of various bioactive molecules in which human macular carotenoids are gaining significant attention. Among 750 natural carotenoids, lutein, zeaxanthin and their oxidative metabolites are selectively accumulated in the macular region of living beings. Due to their vast applications in food, feed, pharmaceutical and nutraceuticals industries, the global market of lutein and zeaxanthin is continuously expanding but chemical synthesis, extraction and purification of these compounds from their natural repertoire e.g., plants, is somewhat costly and technically challenging. In this regard microbial as well as microalga…
Concevoir un living book en sciences humaines et sociales : retour d’expérience
2020
The “living book”, a new format from the Life Sciences, made it possible to take stock of a subject, to highlight a corpus of images, an advanced research and a bibliography. This hybrid, Web-oriented format is re-positioning the producer, the user and the technology. In a simple, lean and rigorous apparatus, it allows to organise a diverse and growing mass of documentation (texts, images, audio, websites). It proposes new ways of writing (collaboration, short texts, summaries) and structuring the contents. It promotes networking and contacts with libraries and museums while drawing attention to formats, licences and rights. It suggests individualised reading paths, reaching diverse audienc…
A comparative analysis to study editing of small noncoding BC200- and Alu transcripts in brain of prion-inoculated rhesus monkeys (M. Mulatta).
2012
Small retroelements (short interspersed elements, abbreviated SINEs) are abundant in vertebrate genomes. Using RNA isolated from rhesus monkey cerebellum and buffy coat, reverse-transcription polymerase chain reaction (RT PCR) was applied to clone cDNA of BC200 and Alu RNAs. Transcripts containing Alu-SINE sequences may be subjected to extensive RNA editing by ADAR (adenosine deaminases that act on RNA) deamination. Abundance of Alu transcripts was determined with real-time RT PCR and was significantly higher than BC200 (brain cytoplasmic) in cerebellum. BC200 transcripts were absent from buffy coat cells. Availability of the rhesus genome sequence allowed the BC200 transcripts to be mapped…
RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase
1998
The purpose of this chapter is to introduce beginners in molecular biology to RNA transcription by phage T7 DNA-dependent RNA polymerase. The work outlined here includes the transcription procedure of plasmid vectors or PCR-amplified DNA templates, the purification and identification of RNA products by sequencing with reverse transcriptase.
Phosphorylation of CENP-A on serine 7 does not control centromere function.
2019
CENP-A is the histone H3 variant necessary to specify the location of all eukaryotic centromeres via its CENP-A targeting domain and either one of its terminal regions. In humans, several post-translational modifications occur on CENP-A, but their role in centromere function remains controversial. One of these modifications of CENP-A, phosphorylation on serine 7, has been proposed to control centromere assembly and function. Here, using gene targeting at both endogenous CENP-A alleles and gene replacement in human cells, we demonstrate that a CENP-A variant that cannot be phosphorylated at serine 7 maintains correct CENP-C recruitment, faithful chromosome segregation and long-term cell viab…
Acquired IFNγ resistance impairs anti-tumor immunity and gives rise to T-cell-resistant melanoma lesions
2016
Melanoma treatment has been revolutionized by antibody-based immunotherapies. IFNγ secretion by CD8+ T cells is critical for therapy efficacy having anti-proliferative and pro-apoptotic effects on tumour cells. Our study demonstrates a genetic evolution of IFNγ resistance in different melanoma patient models. Chromosomal alterations and subsequent inactivating mutations in genes of the IFNγ signalling cascade, most often JAK1 or JAK2, protect melanoma cells from anti-tumour IFNγ activity. JAK1/2 mutants further evolve into T-cell-resistant HLA class I-negative lesions with genes involved in antigen presentation silenced and no longer inducible by IFNγ. Allelic JAK1/2 losses predisposing to …
Deploying Genome Editing Tools for Dissecting the Biology of Nut Trees
2019
Walnuts are among the most important nut crops grown in temperate regions of the world. Commercial production in California, and increasingly worldwide, relies on only few clonally grafted scion genotypes, particularly “Chandler,” and more recently clonally propagated disease-resistant rootstocks. Diseases, nematodes, insect pests, abiotic stresses, and other nutritional and environmental factors, can reduce walnut productivity and quality, affecting grower profitability. The California Walnut Breeding Program at UC Davis has developed and released scion cultivars and rootstocks to help address some of these problems. Sequencing of the walnut genome is expected to speed walnut breeding by f…
Implications of the EFSA Scientific Opinion on Site Directed Nucleases 1 and 2 for Risk Assessment of Genome-Edited Plants in the EU
2021
Genome editing is a set of techniques for introducing targeted changes in genomes. It may be achieved by enzymes collectively called site-directed nucleases (SDN). Site-specificity of SDNs is provided either by the DNA binding domain of the protein molecule itself or by RNA molecule(s) that direct SDN to a specific site in the genome. In contrast to transgenesis resulting in the insertion of exogenous DNA, genome editing only affects specific endogenous sequences. Therefore, multiple jurisdictions around the world have exempted certain types of genome-edited organisms from national biosafety regulations completely, or on a case-by-case basis. In the EU, however, the ruling of the Court of J…
The success of Agronomy for Sustainable Development: from IF 0.566 rank 29/53 to IF 2.9 rank 4/75
2012
Scientific journals are facing an increasing competition directly impacting publishers and editorial boards. In addition, access to scientific information and reading patterns have deeply changed. From 2003 the journal Agronomy for Sustainable Development (ASD) has adapted its editorial policy to this highly competitive and moving environment in order to stand out and improve its impact (1). The scope, language and title of the journal were modified. A preselection step was introduced, several calls for reviews were made and spin-off books were published (2). Particular attention was given to abstracts, titles, colour figures and photos. A sister book series named Sustainable Agriculture Re…