Search results for "Enterobacter"
showing 10 items of 183 documents
Epidemiology ofSalmonella typhimurium: ribosomal DNA analysis of strains from human and animal sources
1993
SUMMARYSalmonella typhimuriumis the most frequently identified serovar ofSalmonellain Italy. This serovar is characterized by the widespread dissemination among human and non-human sources of phenotypically and genetically well-differentiated clones.In this study 457 strains ofS. typhimuriumisolated in Italy in the years 1982–91 from human and animal sources were submitted to characterization by the rDNA fingerprinting technique. Application of this typing method, after digestion of chromosomal DNA withHincII endonuclease, confirmed the greatest genetic differentiation of clones ofS. typhimurium, allowing reliable identification of 45 rDNA patterns linked into 9 major clusters. rDNA pattern…
rRNA probing of chromosomal DNA of epidemic and sporadic isolates of Salmonella enterica subsp. Enterica serovar kottbus from Northern and Southern I…
1990
Fifty-two strains of Salmonella enterica subsp. enterica serovar Kottbus, identified at the Centres of Enterobacteriaceae of Northern and Southern Italy, were investigated by molecular genetic methods. Thirteen isolates were recovered during two food-poisoning outbreaks that occurred in May 1987 in Lombardy. The rDNA gene restriction patterns, obtained by probing endonuclease cleaved chromosomal DNA with photobiotin labeled Escherichia coli rRNA, revealed some heterogeneity among strains isolated from Southern Italy, whereas Northern Italy isolates exhibited virtually identical banding patterns.
Comparison of Four Commercial DNA Extraction Kits for PCR Detection of Listeria monocytogenes, Salmonella, Escherichia coli O157:H7, and Staphylococc…
2008
Four commercial DNA extraction methods, PrepMan Ultra (Applied Biosystems), InstaGene Matrix (BioRad), DNeasy Tissue kit (Qiagen), and UltraClean (MoBio), were tested for PCR detection of Listeria monocytogenes, Escherichia coli O157: H7, Salmonella, and Staphylococcus aureus in fresh, minimally processed vegetables. For comparative purposes, sensitivity assays with specific PCRs were carried out after DNA extraction with the four methods in green pepper, broccoli, and onion artificially inoculated with the four pathogens separately. As confirmed by statistical analysis, the DNeasy Tissue kit rendered the highest sensitivity values in the three matrices assayed for Salmonella, L. monocytoge…
The All-Rounder Sodalis: A New Bacteriome-Associated Endosymbiont of the Lygaeoid Bug Henestaris halophilus (Heteroptera: Henestarinae) and a Critica…
2017
International audience; Hemipteran insects are well-known in their ability to establish symbiotic relationships with bacteria. Among them, heteropteran insects present an array of symbiotic systems, ranging from the most common gut crypt symbiosis to the more restricted bacteriome-associated endosymbiosis, which have only been detected in members of the superfamily Lygaeoidea and the family Cimicidae so far. Genomic data of heteropteran endosymbionts are scarce and have merely been analyzed from the Wolbachia endosymbiont in bed bug and a few gut crypt-associated symbionts in pentatomoid bugs. In this study, we present the first detailed genomic analysis of a bacteriome-associated endosymbi…
Antimicrobial Lessons From a Large Observational Cohort on Intra-abdominal Infections in Intensive Care Units
2021
Severe intra-abdominal infection commonly requires intensive care. Mortality is high and is mainly determined by disease-specific characteristics, i.e. setting of infection onset, anatomical barrier disruption, and severity of disease expression. Recent observations revealed that antimicrobial resistance appears equally common in community-acquired and late-onset hospital-acquired infection. This challenges basic principles in anti-infective therapy guidelines, including the paradigm that pathogens involved in community-acquired infection are covered by standard empiric antimicrobial regimens, and second, the concept of nosocomial acquisition as the main driver for resistance involvement. I…
EUCAST un CLSI metožu salīdzinājums paplašināta spektra βeta-laktamāžu noteikšanai Enterobacteriaceae dzimtas klīniskajos izolātos
2015
Darba mērķis bija salīdzināt EUCAST un CLSI antimikrobās jutības noteikšanas metodes paplašināta spektra β-laktamāžu noteikšanai Enterobacteriaceae dzimtas klīniskajos izolātos. Darba teorētiskajā daļā tika apskatīti biežākie ESBL producējošie Enterobacteriaceae dzimtas pārstāvji, antibakteriālo līdzekļu un rezistences mehānismu raksturojums. Dati iegūti 2014.-2015. g., strādājot klīniskajā mikrobioloģijas laboratorijā. Identificēti un pētīti 269 baktēriju izolāti. Pēc EUCAST standarta 55 izolātiem mainījās antibiogrammas rezultāti. Ceftazidīmam un cefotaksīmam samazinot antibakteriālā līdzekļa koncentrāciju pēc EUCAST standarta, tie tiek padarīti jutīgāki pret ESBL noteikšanu. Pateicoties …
Paplašināta spektra beta laktamāžu noteikšana enterobacteriaceae dzimtas baktērijās
2020
Enterobacteriaceae dzimta ir plaši pārstāvēta mikroorganismu dzimta, kuru veido medicīniski nozīmīgas gramnegatīvas nūjiņas. Šīs dzimtas pārstāvji izraisa infekciju saslimšanas gan vispārējā populācijā, gan medicīnas iestādēs (nozokomiālas infekcijas), skarot kā veselus cilvēkus, tā cilvēkus jau ar esošu saslimšanu. Tās pārstāvji- E.coli un K.pneumoniae, ir vieni no biežākajamiem smagu nozokomiālu infekciju izraisītājiem pasaulē. Arvien pieaug Enterobacteriaceae rezistence pret antibakteriālajiem līdzekļiem, tai skaitā pret paplašināta spektra beta laktāmu antibiotikām. Tas būtiski palielina hospitalizācijas ilgumu, finansiālo slogu kā pacientiem, tā valstij un dēļ tā pieaug pacientu mirstī…
Ūdeņraža mikrobioloģiska producēšana, izmantojot Enterobacter aerogenes, un uzkrāšana AB5 tipa sakausējumā
2017
Ūdeņradis ir atjaunojams un tīrs enerģijas nesējs, kuru var iegūt mikrobioloģiski fermentējot glicerīnu. Šī darba mērķis bija pētīt Enterobacter aerogenes spēju producēt ūdeņradi, fermentējot glicerīnu, kā arī hidrīdu veidojošu AB5 sakausējumu spēju uzkrāt ūdeņradi, tiem atrodoties barotnē ar baktērijām. Barotņu gāzes fāzes sastāvu noteica ar masspektrometru un pārliecinājās par Enterobacter aerogenes spēju producēt ūdeņradi, fermentējot glicerīnu. Tika aprēķināts ūdeņraža produkcijas iznākums 0,15 moli H2 uz molu glicerīna. Konstatēja LaNi5 sakausējuma pulvera inhibējošo ietekmi uz Enterobacter aerogenes gāzu produkciju. Jaukta sastāva AB5 pulveris gāzu produkciju palielināja. Nosakot LaNi…
Superinfections caused by carbapenem-resistant Enterobacterales in hospitalized patients with COVID-19: a multicentre observational study from Italy …
2022
Abstract Objectives To describe clinical characteristics and outcomes of COVID-19 patients who developed secondary infections due to carbapenem-resistant Enterobacterales (CRE). Methods Retrospective observational study including COVID-19 patients admitted to 12 Italian hospitals from March to December 2020 who developed a superinfection by CRE. Superinfection was defined as the occurrence of documented bacterial infection >48 h from admission. Patients with polymicrobial infections were excluded. Demographic, clinical characteristics and outcome were collected. Isolates were classified as KPC, metallo-β-lactamase (MBL) and OXA-48-producing CRE. A Cox regression analysis was performe…
Zanieczyszczenie bioaerozolu w zakładzie cukierniczym bakteriami rodziny Enterobacteriaceae
2018
Mikroorganizmy w powietrzu środowiska produkcji stanowią jeden z głównych czynników prowadzących do zanieczyszczenia produktu lub obniżenia jego jakości. Dobra praktyka produkcyjna zakłada monitorowanie czystości sanitarnej środowiska produkcji. Istotne jest oszacowanie źródła zanieczyszczenia oraz wpływu warunków mikroklimatycznych na zmiany liczebności mikroorganizmów w powietrzu. Celem przeprowadzonych badań była ocena udziału bakterii rodziny Enterobacteriaceae w ogólnej liczbie mikroorganizmów w bioaerozolu hal produkcyjnych w trakcie produkcji. Badania przeprowadzono w zakładzie cukierniczym w trakcie produkcji, w dwóch halach technologicznych (H.1, H.2). W każdej z hal o zróżnicowany…