Search results for "Extraction"

Multimycotoxin analysis in water and fish plasma by liquid chromatography-tandem mass spectrometry

2015

High performance liquid chromatography-mass spectrometry was used for the determination of 15 mycotoxins in water and fish plasma samples, including aflatoxins, fumonisins, ochratoxin A, sterigmatocistin, fusarenon-X and emerging Fusarium mycotoxins. In this work, dispersive liquid-liquid microextraction (DLLME) was assessed as a sample treatment for the simultaneous extraction of mycotoxins. Results showed differences in recovery assays when different extraction solvents were employed. Ethyl acetate showed better recoveries for the major part of mycotoxins analyzed, except for aflatoxins B2, G1 and G2, which showed better recoveries when employing chloroform as extractant solvent. Fumonisi…

Real-time PCR based procedures for detection and quantification of Aspergillus carbonarius in wine grapes

2008

9 pages, 4 tables, 1 figure.

Comparison of Different Analytical Methods for Determination of Type B Trichothecenes in Wheat and Ochratoxin A in Beer

2008

Direct Analysis of Psilocin and Muscimol in Urine Samples Using Single Drop Microextraction Technique In-Line with Capillary Electrophoresis

2020

The fully automated system of single drop microextraction coupled with capillary electrophoresis (SDME-CE) was developed for in-line preconcentration and determination of muscimol (MUS) and psilocin (PSC) from urine samples. Those two analytes are characteristic active metabolites of Amanita and Psilocybe mushrooms, evoking visual and auditory hallucinations. Study analytes were selectively extracted from the donor phase (urine samples, pH 4) into the organic phase (a drop of octanol layer), and re-extracted to the acidic acceptor (background electrolyte, BGE), consisting of 25 mM phosphate buffer (pH 3). The optimized conditions for the extraction procedure of a 200 &micro

Bimodal copper oxide nanoparticles doped phase for the extraction of highly polar compounds by in-tube solid-phase microextraction coupled on-line to…

2020

Abstract Polymers obtained from tetraethyl orthosilicate (TEOS) and triethoxymethylsilane (MTEOS) have been functionalized with different metal and metal oxide nanoparticles (NPs), and used as coatings of extractive capillaries for the extraction of polar compounds by in-tube solid-phase microextraction (IT-SPME) coupled on-line to nano-liquid chromatography (nano-LC). The extraction capabilities of the new phases have been studied using several triazinic herbicides with log of octanol/water partition coefficients (Kow) ranging from -0.7 to 3.21 under reversed phase chromatographic conditions. Best extraction efficiencies for the most polar compounds (log Kow ≤ 2.3) were typically obtained …

Regeneration of total RNA purification silica-based columns

2010

Silica-based columns are largely used in RNA purification, allowing fast extractions and good yields of high quality nucleic acid, but their major limitation is the high cost. The reuse of such columns, although desirable, is not recommended because of residual amounts of material from the previous sample trapped in the column matrix, which might be released during further purification. Thus, recycling does need previous complete removal of any detectable RNA trace, but to date no protocol which allows decontamination and reuse is available.We report a very rapid decontamination procedure, based on treatment with warm alkaline solution containing Triton X-100, which ensures no RNA carry-ove…

C18 alkyl-modified silica: A suitable tool for olive biophenol green extraction

2017

Abstract A clean and straightforward methodology to assess the value of olive mill wastewater (OMWW) samples as well as to obtain biophenol-rich oils that can find employment in a variety of cosmetic, nutraceutical and pharmaceutical products is described. The method employs chromatographic spherical organosilica microparticles, coupled to advanced analytical techniques. OMWW samples obtained in Sicily from two different organically grown cultivars during the exceptional 2015/2016 olive oil season were analyzed, leading us to establish the conditions for optimal extraction of hydroxytyrosol and tyrosol.

Phenolic profiles of olive mill wastewaters treated by membrane filtration systems

2014

BACKGROUND Olive mill wastewater is an important by-product obtained after olive oil extraction. In this investigation, the phenolic composition of olive mill wastewater treated with a semi-industrial membrane filtration system, including ultrafiltration and reverse osmosis modules, was studied. In particular, untreated olive mill wastewater was compared with the permeate and the concentrate fractions of each filtration step. RESULTS 3,4-(dihydroxyphenyl) ethanol and p-(hydroxyphenyl) ethanol were the main compounds of all olive mill wastewater analyzed. A total of 32 compounds, detected at 240 or 280 nm by HPLC-DAD, were considered for phenol quantification. Ultrafiltration reduced phenol …

A Circular Economy Approach to Fish Oil Extraction

2019

Fish oil rich in polyunsaturated omega-3 fatty acids is extracted in high yield from anchovy filleting waste using d-limonene as green biosolvent in a simple solid-liquid extraction performed by mechanically stirring and maceration followed by limonene removal via evaporation under reduced pressure. As limonene is renewably obtained from waste orange peel, this method closes the materials cycle and establishes a circular economy process to obtain high quality fish oil from biowaste available worldwide in several million t/year amount. Significant economic, social and environmental benefits are anticipated.

Medication-related osteonecrosis of the jaw in a cancer patient receiving lenvatinib.

2018

Medication-related osteonecrosis of the jaw (MRONJ) is an adverse drug reaction that affects the mandible and maxilla of patients exposed to bone-targeting agents such as anti-resorptive and anti-angiogenic agents. Several MRONJ cases have been reported after dental extractions in patients under treatment with anti-angiogenic agents, including receptor activator of nuclear factor κB ligand (RANKL) inhibitor, anti-vascular endothelial growth factor (anti-VEGF) monoclonal antibody, mammalian target of rapamycin (mTOR) inhibitors, and tyrosine kinase inhibitors (TKIs). The aim of this article was to describe an original case of lenvatinib-related osteonecrosis of the jaw in a patient affected …