6533b7d7fe1ef96bd1267bba

RESEARCH PRODUCT

Direct Analysis of Psilocin and Muscimol in Urine Samples Using Single Drop Microextraction Technique In-Line with Capillary Electrophoresis

Anna PoliwodaKatarzyna ZielińskaPiotr Wieczorek

subject

OctanolAnalyteLiquid Phase MicroextractionCalibration curveAmanitacapillary electrophoresisPharmaceutical ScienceElectrolyteUrinesingle drop microextraction01 natural sciencesArticleAnalytical Chemistrylcsh:QD241-44103 medical and health scienceschemistry.chemical_compound0302 clinical medicineCapillary electrophoresislcsh:Organic chemistryLimit of DetectionDrug DiscoverymedicineHumans030216 legal & forensic medicinePhysical and Theoretical ChemistrypsilocinChromatographyChemistrygreen chemistry010401 analytical chemistryOrganic ChemistryElectrophoresis CapillaryHydrogen-Ion ConcentrationmuscimolurinePsilocybin0104 chemical sciencesDilutionChemistry (miscellaneous)PsilocinCalibrationHallucinogensSolventsMolecular MedicinePsilocybemedicine.drug

description

The fully automated system of single drop microextraction coupled with capillary electrophoresis (SDME-CE) was developed for in-line preconcentration and determination of muscimol (MUS) and psilocin (PSC) from urine samples. Those two analytes are characteristic active metabolites of Amanita and Psilocybe mushrooms, evoking visual and auditory hallucinations. Study analytes were selectively extracted from the donor phase (urine samples, pH 4) into the organic phase (a drop of octanol layer), and re-extracted to the acidic acceptor (background electrolyte, BGE), consisting of 25 mM phosphate buffer (pH 3). The optimized conditions for the extraction procedure of a 200 &micro

yearjournalcountryeditionlanguage
2020-03-29Molecules
10.3390/molecules25071566https://www.mdpi.com/1420-3049/25/7/1566